Effect of Etanol-Water Ratio as Extraction Solvent towards the Quality of Meniran Herb Extract (Phyllanthus niruri Linn.)



Effects of ratios of ethanol-water as extracting solvent in obtaining extractable material, phenolic compounds and antioxidant activity from the herb of Phyllanthus niruri Linn. have been investigated. The ratios tested were 100:0, 80:20, 70:30, 60:40 and 50:50. The results revealed that the ratio of ethanol-Water used gave significant effect on extractable material, phenolic compounds and antioxidant activity (p<0.05). Among the ratios tested, the best result was obtained by ethanol-water of 60:40 as extracting solvent for the herb of Phyllanthus niruri Linn. to obtain phenolic compound which has antioxidant activity.


1. Sudibyo M. Alam Sumber Kesehatan: Manfaat dan Kegunaan. Jakarta: Balai Pustaka; 1998.

2. Naik AD, and Juvekar AR. Effects of alkaloidal extract of Phyllantus niruri on HIV replication, Indian I Med Sci. 2003, 57(9): 387-93.

3. Notka F, Meier GR, and Wagner R. Inhibition of wild-type human immunodeficiency virus and reverse transcriptase inhibitor-resistant variants by Phyllanthus amarus Antiviral Research. 2003. 58(2): 175-86.

4. Bhattacharjee R, Sil PC. The protein fraction of Phyllanthus niruri plays a protective role against acetaminophen induced hepatic disorder via its antioxidant properties. Phytother Res. 2006. 20(7):595-601.

5. Chatterjee M, and Sil PC. Protective role of Phyllanthus niruri against nimesulide induced hepatic damage, Indian J Clin Biochem. 2007. 22(1): 109-16.

6. Iqbal MJ, Dewam FZ, Chowdhury SAR, Mamun MIR, Moshiuzzaman M and Begum M. Pre-treatment by n-hexane extract of Phyllanthus niruri can alleviate paracetamol-induced damage ofthe rat liver. Bangladesh J Pharmacol. 2007. 2: 43-8.

7. Munjrekar AP, Jisha V, Bag PP, Adhikary B, Pai MM, Hegde A and Nandini M. Effect of Phyllanthus niruri Linn. treatment on liver, kidney and testes in CCI4 induced hepatotoxic rats. Indian J Exp Biol. 2008. 46: 514-20.

8. Freitas AM, Schor N, and Bolm MA.The effect of Phyllanrhus niruri on urinary inhibitors of calcium oxalate crystallization and other factors associated with renal stone formation. BJU Int. 2002. 89(9): 829-34.

9. Nwanjo HU. Studies on the effect of aquous extract of Phyllanthus niruri leaf on plasma glucose level and some hepatospecific markers in diabetic Wistar rats. Intemet J Lab Med. 2009. 2(2): 1-9.

10. Ahmeda A, Ismail Z, and Gabriel A. Antioxidants properties of Phyllanthus niruri (Dukung Anak) Extracts. Malay J of Sci. 2005. 24(1): 195-200.

11. Ditjen POM. Materia Medika Indonesia. Jilid II. Jakarta: Departemen Kesehatan Republik Indonesia; 1978.

12. Bagalkotkar G, Sagineedu SR, Saad MS, and Stanslas J , Phytochemicals from Phyllanthus niruri Linn. and their pharmacological properties: a review. J Pharm Pharmac. 2006. 58(12): 1559-70.

13. Gaedcke F, Steinhoff B and Blasius H. Herbal Medicinal Products: Scientiiic and Regulatory Basis for Development, Quality Assurance and
Marketing Authorisation. Stuttgart: Medpharm Scientific Publisher; 2003.

14. Badan POM. Monografi Ekstrak Turnbuhan Obat Indonesia, Vol 1. Jakarta: Badan Pengawas Obat dan Makanan Republik Indonesia; 2004.

15. Ditjen POM. Parameter Standar Umum Ekstrak Tumbuhan Obat. Jakarta: Departemen Kesehatan Republik Indonesia; 2001.

16. WHO. Quality control Methods for medicinal plant materials, Geneva: World Health Organization; 1998.

17. Pourmorad F, Hosseinimehr SJ and Shahabimajd N. Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. Afr J Biotechnol. 2006. 5(11): 1142-45.

18. Mosquera OM, Correa YM, and Nino J. Antioxidant activity of plants extract from Colombian flora. Braz J Pharmacogn. 2008. 19(2A): 382-87.

19. Mitra S, and Brukh R. Sample Preparation: An Analytical Perspective, in Sample Preparation Techniques in Analytical Chemistry, (Editor: Mitra S.). New York; John Wiley & Sons Inc.; 2003, 1-36.

20. Harmita. Petunjuk pelaksanaan validasi metode dan cara perhitungannya. Majalah Ilmu Kefarmasian 2004. 1(3):117-35.
How to Cite
RIVAI, HARRISUL et al. Effect of Etanol-Water Ratio as Extraction Solvent towards the Quality of Meniran Herb Extract (Phyllanthus niruri Linn.). JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 8, n. 2, p. 69-73, sep. 2010. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/330>. Date accessed: 30 may 2023.