Effect of Etanol-Water Ratio as Extraction Solvent towards the Quality of Meniran Herb Extract (Phyllanthus niruri Linn.)
Abstract
Effects of ratios of ethanol-water as extracting solvent in obtaining extractable material, phenolic compounds and antioxidant activity from the herb of Phyllanthus niruri Linn. have been investigated. The ratios tested were 100:0, 80:20, 70:30, 60:40 and 50:50. The results revealed that the ratio of ethanol-Water used gave significant effect on extractable material, phenolic compounds and antioxidant activity (p<0.05). Among the ratios tested, the best result was obtained by ethanol-water of 60:40 as extracting solvent for the herb of Phyllanthus niruri Linn. to obtain phenolic compound which has antioxidant activity.
References
1. Sudibyo M. Alam Sumber Kesehatan: Manfaat dan Kegunaan. Jakarta: Balai Pustaka; 1998.
2. Naik AD, and Juvekar AR. Effects of alkaloidal extract of Phyllantus niruri on HIV replication, Indian I Med Sci. 2003, 57(9): 387-93.
3. Notka F, Meier GR, and Wagner R. Inhibition of wild-type human immunodeficiency virus and reverse transcriptase inhibitor-resistant variants by Phyllanthus amarus Antiviral Research. 2003. 58(2): 175-86.
4. Bhattacharjee R, Sil PC. The protein fraction of Phyllanthus niruri plays a protective role against acetaminophen induced hepatic disorder via its antioxidant properties. Phytother Res. 2006. 20(7):595-601.
5. Chatterjee M, and Sil PC. Protective role of Phyllanthus niruri against nimesulide induced hepatic damage, Indian J Clin Biochem. 2007. 22(1): 109-16.
6. Iqbal MJ, Dewam FZ, Chowdhury SAR, Mamun MIR, Moshiuzzaman M and Begum M. Pre-treatment by n-hexane extract of Phyllanthus niruri can alleviate paracetamol-induced damage ofthe rat liver. Bangladesh J Pharmacol. 2007. 2: 43-8.
7. Munjrekar AP, Jisha V, Bag PP, Adhikary B, Pai MM, Hegde A and Nandini M. Effect of Phyllanthus niruri Linn. treatment on liver, kidney and testes in CCI4 induced hepatotoxic rats. Indian J Exp Biol. 2008. 46: 514-20.
8. Freitas AM, Schor N, and Bolm MA.The effect of Phyllanrhus niruri on urinary inhibitors of calcium oxalate crystallization and other factors associated with renal stone formation. BJU Int. 2002. 89(9): 829-34.
9. Nwanjo HU. Studies on the effect of aquous extract of Phyllanthus niruri leaf on plasma glucose level and some hepatospecific markers in diabetic Wistar rats. Intemet J Lab Med. 2009. 2(2): 1-9.
10. Ahmeda A, Ismail Z, and Gabriel A. Antioxidants properties of Phyllanthus niruri (Dukung Anak) Extracts. Malay J of Sci. 2005. 24(1): 195-200.
11. Ditjen POM. Materia Medika Indonesia. Jilid II. Jakarta: Departemen Kesehatan Republik Indonesia; 1978.
12. Bagalkotkar G, Sagineedu SR, Saad MS, and Stanslas J , Phytochemicals from Phyllanthus niruri Linn. and their pharmacological properties: a review. J Pharm Pharmac. 2006. 58(12): 1559-70.
13. Gaedcke F, Steinhoff B and Blasius H. Herbal Medicinal Products: Scientiiic and Regulatory Basis for Development, Quality Assurance and
Marketing Authorisation. Stuttgart: Medpharm Scientific Publisher; 2003.
14. Badan POM. Monografi Ekstrak Turnbuhan Obat Indonesia, Vol 1. Jakarta: Badan Pengawas Obat dan Makanan Republik Indonesia; 2004.
15. Ditjen POM. Parameter Standar Umum Ekstrak Tumbuhan Obat. Jakarta: Departemen Kesehatan Republik Indonesia; 2001.
16. WHO. Quality control Methods for medicinal plant materials, Geneva: World Health Organization; 1998.
17. Pourmorad F, Hosseinimehr SJ and Shahabimajd N. Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. Afr J Biotechnol. 2006. 5(11): 1142-45.
18. Mosquera OM, Correa YM, and Nino J. Antioxidant activity of plants extract from Colombian flora. Braz J Pharmacogn. 2008. 19(2A): 382-87.
19. Mitra S, and Brukh R. Sample Preparation: An Analytical Perspective, in Sample Preparation Techniques in Analytical Chemistry, (Editor: Mitra S.). New York; John Wiley & Sons Inc.; 2003, 1-36.
20. Harmita. Petunjuk pelaksanaan validasi metode dan cara perhitungannya. Majalah Ilmu Kefarmasian 2004. 1(3):117-35.
2. Naik AD, and Juvekar AR. Effects of alkaloidal extract of Phyllantus niruri on HIV replication, Indian I Med Sci. 2003, 57(9): 387-93.
3. Notka F, Meier GR, and Wagner R. Inhibition of wild-type human immunodeficiency virus and reverse transcriptase inhibitor-resistant variants by Phyllanthus amarus Antiviral Research. 2003. 58(2): 175-86.
4. Bhattacharjee R, Sil PC. The protein fraction of Phyllanthus niruri plays a protective role against acetaminophen induced hepatic disorder via its antioxidant properties. Phytother Res. 2006. 20(7):595-601.
5. Chatterjee M, and Sil PC. Protective role of Phyllanthus niruri against nimesulide induced hepatic damage, Indian J Clin Biochem. 2007. 22(1): 109-16.
6. Iqbal MJ, Dewam FZ, Chowdhury SAR, Mamun MIR, Moshiuzzaman M and Begum M. Pre-treatment by n-hexane extract of Phyllanthus niruri can alleviate paracetamol-induced damage ofthe rat liver. Bangladesh J Pharmacol. 2007. 2: 43-8.
7. Munjrekar AP, Jisha V, Bag PP, Adhikary B, Pai MM, Hegde A and Nandini M. Effect of Phyllanthus niruri Linn. treatment on liver, kidney and testes in CCI4 induced hepatotoxic rats. Indian J Exp Biol. 2008. 46: 514-20.
8. Freitas AM, Schor N, and Bolm MA.The effect of Phyllanrhus niruri on urinary inhibitors of calcium oxalate crystallization and other factors associated with renal stone formation. BJU Int. 2002. 89(9): 829-34.
9. Nwanjo HU. Studies on the effect of aquous extract of Phyllanthus niruri leaf on plasma glucose level and some hepatospecific markers in diabetic Wistar rats. Intemet J Lab Med. 2009. 2(2): 1-9.
10. Ahmeda A, Ismail Z, and Gabriel A. Antioxidants properties of Phyllanthus niruri (Dukung Anak) Extracts. Malay J of Sci. 2005. 24(1): 195-200.
11. Ditjen POM. Materia Medika Indonesia. Jilid II. Jakarta: Departemen Kesehatan Republik Indonesia; 1978.
12. Bagalkotkar G, Sagineedu SR, Saad MS, and Stanslas J , Phytochemicals from Phyllanthus niruri Linn. and their pharmacological properties: a review. J Pharm Pharmac. 2006. 58(12): 1559-70.
13. Gaedcke F, Steinhoff B and Blasius H. Herbal Medicinal Products: Scientiiic and Regulatory Basis for Development, Quality Assurance and
Marketing Authorisation. Stuttgart: Medpharm Scientific Publisher; 2003.
14. Badan POM. Monografi Ekstrak Turnbuhan Obat Indonesia, Vol 1. Jakarta: Badan Pengawas Obat dan Makanan Republik Indonesia; 2004.
15. Ditjen POM. Parameter Standar Umum Ekstrak Tumbuhan Obat. Jakarta: Departemen Kesehatan Republik Indonesia; 2001.
16. WHO. Quality control Methods for medicinal plant materials, Geneva: World Health Organization; 1998.
17. Pourmorad F, Hosseinimehr SJ and Shahabimajd N. Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. Afr J Biotechnol. 2006. 5(11): 1142-45.
18. Mosquera OM, Correa YM, and Nino J. Antioxidant activity of plants extract from Colombian flora. Braz J Pharmacogn. 2008. 19(2A): 382-87.
19. Mitra S, and Brukh R. Sample Preparation: An Analytical Perspective, in Sample Preparation Techniques in Analytical Chemistry, (Editor: Mitra S.). New York; John Wiley & Sons Inc.; 2003, 1-36.
20. Harmita. Petunjuk pelaksanaan validasi metode dan cara perhitungannya. Majalah Ilmu Kefarmasian 2004. 1(3):117-35.
Published
2010-09-30
How to Cite
RIVAI, HARRISUL et al.
Effect of Etanol-Water Ratio as Extraction Solvent towards the Quality of Meniran Herb Extract (Phyllanthus niruri Linn.).
JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 8, n. 2, p. 69-73, sep. 2010.
ISSN 2614-6495.
Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/330>. Date accessed: 25 apr. 2024.
Issue
Section
Articles
License
Licencing
All articles in Jurnal Ilmu Kefarmasian Indonesia are an open-access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which permits unrestricted non-commercial used, distribution and reproduction in any medium.
This licence applies to Author(s) and Public Reader means that the users mays :
- SHARE:
copy and redistribute the article in any medium or format - ADAPT:
remix, transform, and build upon the article (eg.: to produce a new research work and, possibly, a new publication) - ALIKE:
If you remix, transform, or build upon the article, you must distribute your contributions under the same license as the original. - NO ADDITIONAL RESTRICTIONS:
You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
It does however mean that when you use it you must:
- ATTRIBUTION: You must give appropriate credit to both the Author(s) and the journal, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
You may not:
- NONCOMMERCIAL: You may not use the article for commercial purposes.
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Tools
