Isolation and Identification of Jatrophone from the Root of Jatropha goossyptfolia and Evaluation of Its Toxicity and Cytotoxicity

  • SAHIDIN I UNIVERSITAS HALUOLEO
  • ARDIANSYAH ARDIANSYAH UNIVERSITAS HALUOLEO
  • MUHAMAD TAHER UNIVERSITY MALAYSIA

Abstract

Isolation and identitication of secondary metabolites from root of Jatropha gossypyolia, and also its biological activity test toward microbes and tumor cells have been conducted, Isolation was carried out by using vacuum liqiud chromatography (VLC) method (adsorbent: silica gel, eluent: mixture of n-hexane, ethylacetate, chloroform, and methanol). The isolate molecular structure Was determined by spectroscopic technique i.e. FTIR, NMR-ID, 13C-DEPT, NMR-2D, comparing the data obtained with related data from literatures. Its biological activity was evaluated against bacteria (Acetobacter sp., Escherecia coli, Staphylococcus aureus, and Streptococcus sp.), fungi (Aspergillus nigcr, Pentcillium sp. [grey], Penicillium sp. [White] and Rhizopus sp.), and murine leukemia P-388 cells. The results showed that the isolate is a white needle crystal which is identified as jatrophone. Jatrophone is very active toward Acetobacter sp. (bacteria) and A. niger (fungi) with growth inhibition Zone of 3,6 cm and 4.4 cm, respectively. Meanwhile, jatrophone has low activity against murine leukemia P-388 cells with IC50, > 100 µg/mL.

References

1. Tarigan J. Ester Asam Lemak, F-MIPA Universitas Sumatera Utara, Medan, 2002.

2. Parsons WT, dan Cuthbertson EG, Noxious Weeds of Australia. Melbourne: lnkata Press;1992. 187.

3. Ogbobe O, Akano V The physico-chemical properties of the seed and seed oil ofJazropha gossypyblia. Plant Foods for Human Nutrition 1993.(43): 197-200.

4. Heyne, K. Tumbuhan Berguna Indonesia, Jilid IH, Jakarta: Badan Litbang Kehutanan; 1987.

5. Lans C, Harper T, Georges K, Bridgewater E. Medicinal and ethnoveterinary remedies of hunters in Trinidad. BMC Complementary and Alternative Medicine. 2001. (1): 1-10

6. Sukumaran D, Parashar BD, Gupta AK, Jeevaratnam K, Prakash S. Molluscicidal Effect ofNicotinanilide and Its Intermediate Compounds against a Freshwater Snail Lymnaea luteola the Vector of Animal Schistosomiasis. Mem Inst Oswaldo Cruz. Rio de Janeiro. 2004. 99(2): 205-10,

7. Dabur R, Gupta A, Mandal TK, Singh DD, Bajpai V, Gurav AM, el al. Antimicrobial Activity Of Some Indian Medicinal Plants. Afr J Trad CAM. 2007. 4(3): 313-18.

8. Sahidin I, Ruslin, Zaeni A, Raharjo S, Inventarisasi, relokasi, dan kajian ilrniah tanaman obat tradisional
masyarakat Sulawesi Tenggara. Laporan Penelitian Universitas Haluoleo, Kendari. 2008. 28-32.

9. Thakurta P, Bhowrnik P, Mukherjee S, Hajra TK, Patra A, Bag PK. Antibacterial, Antisecretory and Antihemorrhagic Activity of Azadirachta indica Used to Treat Cholera and Diarrhea in India. J Ethnopharmacol. 2007. 111: 607-12.

10. Alley MC, Scudiero DA, Monks A, Hursey ML, Czerwinski MJ, Fine DL, Abbott BJ, Mayo JG, Shoemaker RH, dan Boyd MR. Feasibility of drug screening with panels ofhuman tumor cell lines using a microculturetetrazolium assay, Cancer Research 1988. 48: 589-601.

11. Pertino M, Hirschmann GS, Santos LS, Rodriguez JA, Theoduloz C, Biotransformation of Jatrophone
by Aspergillis niger ATCC 16404. Z. Naturforsch 62b. 2007. 275-79.

12. Goulart MOF, Sant’Ana AEG, Lima RA, Cavalcante SH. Fitoconstiuintes Quimicos Isolados De Jatropha elliptica. Atribuicao Dos Deslocamentos Quimicos Dos Atomos De Carbon E Hidrogenio Dos Diteipenos Jatrofolonas A E . Laboratorio de Pesquisa em Recursos Naturais. Departamento de Quimica. Universade Federal de Alagoas. 1992. 185-6.
Published
2010-09-30
How to Cite
I, SAHIDIN; ARDIANSYAH, ARDIANSYAH; TAHER, MUHAMAD. Isolation and Identification of Jatrophone from the Root of Jatropha goossyptfolia and Evaluation of Its Toxicity and Cytotoxicity. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 8, n. 2, p. 80-84, sep. 2010. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/334>. Date accessed: 05 nov. 2024.
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