Formulation of Lozenges Containing Green Tea Extract (Camellia sinensis L. Kuntz) with Additon of Vitamin C or Grape Seed Extract (Vitis vinifera L.) and Their Antioxidant Activities

  • SWASONO R. TAMAT UNIVERSITAS PANCASILA
  • LEONARDUS B.S. KARDONO LEMBAGA ILMU PENGETAHUAN INDONESIA
  • DEWITA AGUS R & D MANAJER PT. MUSTIKA RATU, Tbk. JAKARTA

Abstract

Formulation of lozenges containing: A) green tea extract; B) green tea extract with sodium ascorbate; and C) green tea extract with grape seed extract have been carried out. Antioxidant activities were compared to the activity of raw active materials. Lozenges were tested for physical characteristics, hedonic values, dissolution rate, and stability during storage, Physical tests indicated that the lozenges comply with the Farmakope Indonesia IV requirements. Determination of IC50 antioxidant activity using DPPI-I free radical scavenger showed that IC50 of formula A, B, and C were 844.04 µg/mL, 221.80 µg/ mL, and C 57.4 µg/mL, respectively. Meanwhile, IC50 value of green tea extract, sodium ascorbate, and grape seed extract were 4.29 µg/mL, 12.95 µg/mL, and 1.96 µg/ml., respectively. Accelerated stability test were carried out based on the EGCG concentration observed by using HPLC and then calculated by Arrhenius equation showed that the shelf life of lozenges of formula A, B, and C Were 2,11 years; 1.87 years; and 2,73 years, respectively. The result showed that the lozenge containing green tea extract and grape seed extract (formula C) is the best formula based on FI IV requirements, antioxidant activity and lozenges stability.

References

1. Hartoyo A. Teh dan Khasiatnya bagi Kesehatan, Sebuah Tinjauan Ilmiah. Jogjakarta: Penerbit Kanisius; 2003. 16-9, 25-35.

2. Liu Z. Tea Comprehensive Processing in China, Presentation. Hunan Provincial Research Centre of Natural Products, Changsha: Hunan Agricultural University; 2003. 1-3.

3. Gruenwald J . Physician’s Desk Reference for Herbal Medicine. 3rd ed. New Jersey: Thomson PDR at Montvale; 2004, 400-4, 408-14, 975-9.

4. Singleton VL, Oithofer R, Larnuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu Reagent. Methods in Enzymology 1999: 299, 152-78.

5. lnstitute for Nutraceutical Advancement (INA). 2000, Methods Validation Programs: Determination of Total Catechins and Gallic Acid in Green Tea. INA Method 111.000. diambil dari www.ina.organization
Diakses 16 Januari, 2009. 1-7.


6. Hristea, EN, Caproiu MT, Pencu G, et. al. Reaction of 2,2-diphenyl-1-picrylhydrazyl with HO-, O2-, HO-, and HOO- Radical and Anions. lnt J Mol Sci. 2006. 7: 130-143.

7. Potchoo Y, Guissou LP, Lompo M, et al_,Antioxidant Activity of Aqueous Methanol and Ethyl Acetate Extract of Leaves of Annona senegalensis Pers from Togo versus the One Originates from Burkina Faso, Int J Pharmacol. 2008. 1: 120.

8. British Pharmacopoeia Commission, British Pharmacopoeia. London: The Department of Health, Social Services and Public Safety; 2005, 2174-5

9. Rowe RC, Sheskey PJ, Weller PJ. Handbook of Pharmaceutical Excipient. Washington: Pharmaceutical Express and American Pharmaceutical Association; 2003, 596.

10. Departemen Kesehatan RI, Farmakope Indonesia, ed. IV, Jakarta: Depertemen Kesehatan Republik Indonesia; 1995, 39.

11. Departemen Kesehatan RI. Keputusan Menteri Kesehatan RI No: 66 1/Menkes/ SK /VII/ 1994 tentang Persyaratan Obat Tradisional, Jakarta: DepKes RI; 1994.

12. Sinko PJ, Martin. Martins Physical Pharmacy and Pharmaceutical Sciences, 5th ed. Philadelphia: Lippincott Williams & Wilkins; 2005. 397.

13. Lieberman HA, Lachman L. Pharmaceutical Dosage Forms: Tablets, Volume 1, 2nd ed., rev and expanded, New York: Marcel Dekker; 1989. 543-83.

14. Dirjen Bina Pengolahan dan Pengolahan Hasil Pertanian. Buletin Teknopro Hortikultura, Jakarta: Direktorat Pengolahan dan Pemasaran Hasil Hortikulturag 2004. 1-2.

15. Dissolution Disscussion Group Bulletin Board, DDG News and Dissolution Seminars. diambil dari http:// www.dissolution.com /vbulletin/showthread.php. diakses 7 September, 2009. 1.
Published
2010-09-30
How to Cite
TAMAT, SWASONO R.; KARDONO, LEONARDUS B.S.; AGUS, DEWITA. Formulation of Lozenges Containing Green Tea Extract (Camellia sinensis L. Kuntz) with Additon of Vitamin C or Grape Seed Extract (Vitis vinifera L.) and Their Antioxidant Activities. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 8, n. 2, p. 108-117, sep. 2010. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/340>. Date accessed: 24 nov. 2024.
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Vol 8 No 2 (2010): JIFI
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