Sinergisme Fraksi Butanol Metabolit Sekunder Kapang Endofit 1.3.11 dengan Doxorubicin dalam Modulasi Daur Sel T47D dan MCF-7

  • SHIRLY KUMALA UNIVERSITAS PANCASILA
  • EDY MEIYANTO UNIVERSITAS GADJAH MADA
  • MUTHI IKAWATI UNIVERSITAS GADJAH MADA
  • RIRIS ISTIGHFARI JENIE UNIVERSITAS GADJAH MADA

Abstract

The synergic effects of n-butanolic fraction of secondary metabolite of endophytic fungus 1.3.11 (FB) and doxorubicin (Dox) on cell cycle regulation and the expression of Bcl-2 gene expression were investigated on MCF-7 and T47-D cells by iiow cytometry and immunocytochemical techniques respectively. The results showed that after 12 hours of incubation period with FB at its IC50 dose, MCF-7 cell cycle was inhibited at G1 phase while Dox inhibited the cell cycle at G2/M phase. Similar results were observed in T47-D cells when incubated with Dox and FB individually under the same treatment condition. Further treatment was then performed to these cells where both Dox and FB were combined at their IC50 and  lC50 dose and added to incubate with the cells over 12 hours period. Interestingly, the modified treatment combination showed that MCF-7 and T47-D cell cycle regulation were inhibited at G2/M phase. Our immunocytochemical study also showed no significant inhibition suppression of Bel-2 gene expression in both MCF-7 and T47-D cells when compared with their corresponding positive control after treatment with FB and Dox or both combined FB and Dox at 1C50 and  IC50 dosage over 15 hours incubation.

References

1. World Health Grganization, Fact sheet No. 297, Cancer.
2006.

2. Wong HL, Bendayan R, RauthAM, Xue HY Babakhanian K, Wu XY Amechanistic study of enhanced doxorubicin uptake and retention in multidrug resistant breast cancer cells using a polymer-lipid hybrid nanoparticle system. The J of Pharmacology and Experimental Therapeutics. 2006. 3 17(3): 1372-81.

3. Prakash J, Gupta SK. Natural products for chemoprevention. Indian J of Med and Paed Onc. 2004.25(3):37-53.


4. Rahman S, Fukamiya N, Tokuda H, Nishino H, Tagahara K, Lee K, Okano M. Three new quassinoid derivatives and related compounds as antitumor promoters from Brucea javanica. Bull of Chem Soc of Japan. 1999:72.

5. Cuendet M, Christov K, Lantvit DD, Deng SL Hedayat S, Helson L, McChesney JD, Pezzuto JM. Multiple myeloma regression mediated by bruceantin. Clinical Cancer Research. 2004(10):1170-79.

6. Mata-Greenwood E, Cuendet M, Sher D, Gustin D, Stock W, Pezzuto JM. Brusatol-mediated induction of leukemic cell differentiation and G, arrest is associated with down-regulation of c-myc. Leukemia. 2002.16 (11):2275-84.

7. Lau FY, Chui CH, Roberto G, Stanton HLK, Kan KL, Gregory YMC, Raymond SMW. Ivy TNT, Cheng CH, Thomas SKW, Albert SCC, Johnny COT. Antiproliferative and apoptosis-inducing activity of Brucea javanica extract on human carcinoma cells. Int J Mol Med. 2005. 16(6): 1 157-62.

8. Ryoho GTK. Xuan YB, Yasuda S, Shimada K., Nagai S, Ishihama H. Growth inhibition of the emulsion from Brucea javanica to cultured human carcinoma cells. 1994.21(14):2421-25.

9. Kumala S. Isolasi dan penapisan mikroba endofit tanaman Bruceajavanica GJ.) Merr. serta uji sitotoksik metabolit sekunder terhadap beberapa sel kanker seeara in vitro [Disertasi]. Jakarta: Program Studi Biomedik, Program Pascasarjana Fakultas Kedokteran, Universitas Indonesia. 2005. hal. 55-101 .

10. Kumala S, Septisetyani EP, Meiyanto E. Fraksi n-butanol supernatan fermentasi kapang endoiit (isolat 1 .3 . 1 1) buah Makasar meningkatkan efek apoptosis doxorubicin pada sel MCF-7. Indon J Pharm G\/[FI).2009.20(2):42-7.

11. Meiyanto E. Kurkumin sebagai obat kanker: menelusuri mekanisme aksinya. Majalah Farmasi Indonesia. 1999. 10(4):224-36.

12. Anonim. MCF7 cell. Medical dictionary. 2000. Diambil dari www.wrongdiagnosis.com/medical/ mcf7_cell.html. Diakses bulan September 2008.


13. Zampieri PL, Bianchi P, Ruff P, Arbuthnot P. Differential modulation by estradiol of p-glycoprotein drug resistance protein expression in cultured MCF7 and T47D breast cancer cells. Anticancer Res. 2002.22(4):2253-9.

14. Cheepthan N, Phay N, Hugashiyama, Fukushi E, Matsuura H, Mikawa T. Studies on antifungi antibiotic from Ellisiodothis inguinamas L1588-A8.Thai J Biotechnol. 1999.(Sep):37-45.
Published
2010-04-30
How to Cite
KUMALA, SHIRLY et al. Sinergisme Fraksi Butanol Metabolit Sekunder Kapang Endofit 1.3.11 dengan Doxorubicin dalam Modulasi Daur Sel T47D dan MCF-7. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 8, n. 1, p. 1-9, apr. 2010. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/355>. Date accessed: 22 dec. 2024.
Citation Formats
Issue
Vol 8 No 1 (2010): JIFI
Section
Articles
License

Licencing
All articles in Jurnal Ilmu Kefarmasian Indonesia are an open-access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which permits unrestricted non-commercial used, distribution and reproduction in any medium.

This licence applies to Author(s) and Public Reader means that the users mays :

  • SHARE:
    copy and redistribute the article in any medium or format
  • ADAPT:
    remix, transform, and build upon the article (eg.: to produce a new research work and, possibly, a new publication)
  • ALIKE:
    If you remix, transform, or build upon the article, you must distribute your contributions under the same license as the original.
  • NO ADDITIONAL RESTRICTIONS:
    You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

It does however mean that when you use it you must:

  • ATTRIBUTION: You must give appropriate credit to both the Author(s) and the journal, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.

You may not:

  • NONCOMMERCIAL: You may not use the article for commercial purposes.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.