Penandaan Falerin dengan Iodium-131 dan Uji Biodistribusi pada Mencit yang Diinduksi Inflamasi

  • WIDYASTUTI WIDJAKSANA PUSAT RADIOISOTOP dan RADIOFARMAKA ATOM NASIONAL (BATAN)
  • AGUS ARIYANTO PUSAT RADIOISOTOP dan RADIOFARMAKA ATOM NASIONAL (BATAN)
  • GINA MONDRIDA PUSAT RADIOISOTOP dan RADIOFARMAKA ATOM NASIONAL (BATAN)
  • ARINA KUSUMAWARDANI INSTITUTE OF TECHNOLOGY BANDUNG
  • AS' ARI NAWAWI INSTITUTE OF TECHNOLOGY BANDUNG
  • ABDUL MUTALIB PUSAT RADIOISOTOP dan RADIOFARMAKA ATOM NASIONAL (BATAN)

Abstract

Phalerin is an active component of mahkota dewa (Phaleria macrocarpa (Scheff) Boerl) proven to have an anti-inflammation effect. The labeling of phalerin with gamma emitting radionuclides was aimed to study its pharmacokinetic behavior and particularly to trace its metabolites. The labeling with 131I was caried out using iodogen as oxidator. Radiolabeled compound was characterized by high performance liquid chromatography (HPLC) using C- 18 column eluted with methanol 70% and detected with UV detector (λ=291 nm) and by thin layer chromatography (TLC) using silica gel strips eluted with chloroform-methanol (9:2), and labeling efficiency was determined using the same TLC system. Purification of radiolabeled product Was carried out using size exclusion chromatography (Sephadex G-25 column) eluted with 0.05 M phosphate buffer pH 7.4. Biodistributions of 131l-phalerin in various organs of normal and inilammation-induced mice were observed at 1, 4, and 24 hours post-intravenous injection. Radiochemical purity of 131l-phalerin was 90.2 ± 2.8% and increased to 96.0 ± 0.4% after purification. Radioactivities in inflamed tissue at 1, 4, and 24 hours post-injection were respectively 1.6 times, 1.4 times, and 1.3 times higher than that in normal tissue, The results showed a significant uptake of radiolabeled phalerin in inflamed tissue.

References

1. Harmanto N. Mahkota dewa: obat pusaka para dewa. Jakarta: Agromedia; 2001. hal. 9-26.

2. ZhangYB, Xiang JX, Hong ML. Chemical constituents from mahkota dewa. I AsianNatProduct Res. 2006. 8: 119-23.

3. Rahmi K, Siti K, As’ari N. Telaah fitokimia daun mahkota dewa (Phaleria macrocarpa (Scheff.) Boerl.) [skripsi]. Bandung: Sekolah Farmasi ITB; 2003.

4. Mariani R, Wirasutisna KR, As’ari N, I Ketut A. Telaah kandungan kimia dan aktivitas antiradang buah mahkota dewa (Phaleria macrocarpa (Scheff.) Boerl.) [tesis]. Bandung: Sekolah Farmasi ITB; 2005. hal. 25-30.

5. Heri SNS, As’ari N, Mutalib A. Studi pelabelan falerin dengan ml menggunakan metoda komputasi AMl [skripsi]. Bandung: Sekolah Fannasi ITB; 2008.

6. Stephen JM. Radiolabeled antibodies and peptides. “Textbook of radiopharmacy: theory and practice”, 3rd. Edited by Charles B. Sampson. Amsterdam: Gordon and Breach Science Publishers; 1999. p. 63-82.
Published
2010-04-30
How to Cite
WIDJAKSANA, WIDYASTUTI et al. Penandaan Falerin dengan Iodium-131 dan Uji Biodistribusi pada Mencit yang Diinduksi Inflamasi. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 8, n. 1, p. 25-31, apr. 2010. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/363>. Date accessed: 05 nov. 2024.
Citation Formats
Issue
Vol 8 No 1 (2010): JIFI
Section
Articles
License

Licencing
All articles in Jurnal Ilmu Kefarmasian Indonesia are an open-access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which permits unrestricted non-commercial used, distribution and reproduction in any medium.

This licence applies to Author(s) and Public Reader means that the users mays :

  • SHARE:
    copy and redistribute the article in any medium or format
  • ADAPT:
    remix, transform, and build upon the article (eg.: to produce a new research work and, possibly, a new publication)
  • ALIKE:
    If you remix, transform, or build upon the article, you must distribute your contributions under the same license as the original.
  • NO ADDITIONAL RESTRICTIONS:
    You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

It does however mean that when you use it you must:

  • ATTRIBUTION: You must give appropriate credit to both the Author(s) and the journal, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.

You may not:

  • NONCOMMERCIAL: You may not use the article for commercial purposes.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.