Uji Sitotoksisitas Ekstrak Etanol Herba Ceplukan (Physalis angulata Linn.) terhadap Sel T47D secara In Vitro

  • IRA DJAJANEGARA BADAN PENGKAJIAN dan PENERAPAN TEKNOLOGI
  • PRIYO WAHYUDI BADAN PENGKAJIAN dan PENERAPAN TEKNOLOGI

Abstract

Cutleaf groundcheny (Physalis angulata Linn.) is a popular traditional medicine. This experiment was conducted to investigate the possible role of the herbal extract as an anticancer agent using T47D breast cancer cell line. Cytotoxicity assay was caried out by direct counting method. Breastcancer T47D cell lines Were treated with 70% ethanol extract from cutleaf groundcherry fruit with serial dilution of 2000, 1000, 500, 250, 125, 62.5, and 31.25 µg/ml for 24 hours of incubation at 37°C As a positive control doxorubicin was also tested on the breast cancer T47D cell lines with serial dilution of 20, 10, 5, 2.5, 1.25, 0.625, 0.312, and 0.156 µg/ml for 24 hours of incubation at 37°C The amount of living cells were observed and counted, death percentage was then determined and probit analysis was used to determine the LC50 value. The LC50 value for ethanol extract from the fruits was 28,02 µg/ml While doxorubicin as the positive control was 0,113 µg/ml. It is concluded that the cutleaf groundcherry (Physalis angulata) fruits have a cytotoxic activity toward T47D (breast cancer) cell lines which confirmed previous toxicity experiment using brine shrimp lethality test (BSLT) method.

References

1. Ma’at S. Tanarnan obat untuk pengobatan kanker. Jurnal Bahan alam Indonesia. 2003.2(1):158.

2. Kirpal D. In vitro and in vivo growth suppression of MCF-7 human breast cancer by novel photoproducts and tamoxifen. Int J of the Am Cancer Soc. 1994. 74(6):23-45.

3. Djumidi. Inventaris tanaman obat Indonesia. Jilid I. Jakarta: Badan Penelitian dan Pengembangan Kesehatan Depkes RI; 1991. hal. 179-80.


4. Reksoprodjo S. Kumpulan kuliah ilmu bedah. Jakarta: Fakultas Kedokteran Universitas Indonesia Bagian ilmu bedah Fakultas Kedokteran Universitas Indonesia / Rumah Sakit Dr. Cipto Mangun Kusumo; 1995. hal. 342-63, 225-32.

5. Diijen POM Depkes RI. Materia medika Indonesia. J ilid
VI. Jakarta: Dirjen POM Depkes RI; 1995. hal. 195-9.

6. Meiyanto E. Kurkumin sebagai antikanker: menelusuri mekanisme aksinya. Majalah Farmasi Indonesia. 1999. 10(4):224-36.

7. Hudgson EP. Textbook of Modern Toxicology. 2nd ed. Singapore: The McGroW-hill Companies, Inc.; 2000. p. 172-7.

8. Schafer JM, Lee ES, O’Regan RM, Yao K, Jordan VC. Rapid development of tamoxifen-stimulated mutant p53 breast tumors (T47D) in Athymic Mice. Clin Cancer Res. 2000.6:4373-80.

9. Subroto A, Saputro. Gempur penyakit dengan sarang semut. Diambil dari http://www.deherba.com/ p6.htm. Diakses tanggal 4 November 2007.

10. Karwati I. Uji toksisitas ekstrak etanol 70%, fraksi etil asetat, fraksi n-heksan, fraksi etanol, dan fraksi air terhadap herba ceplukan (Physalzs agulata Linn.) [skripsi]. J akartaz Fakultas MIPA UHAMKA; 2006. hal. 35.

11. Doyle A, Griffiths JB. Cell and tissue culture for medical research. New York: John Wiley & Sons, Ltd.; 2000. p. 12-6, 48-9, 409.

12. Harbome. Metode fitokimia. Terbitan kedua.Terjemahan dari: Phytochemical methods. Oleh: Padmawinata, Kosasih, Iwan S. Bandung: Penerbit ITB; hal. 6, 147.

13. Radiat Phys Chem. 1995 .462 161-8.


14. Khoylou F, Naimian F. Radiation synthesis of superabsorbent polyethylene oxide/tragacanth hydrogel. Radiation Physic and Chemistry.2009, (78):195-198.

15. Badan Standarisasi Nasional. SNI 16-6363-2000: Pembalut Wanita. Jakarta. 2000.

16. Soerens, Dave A, Malik S. PATENT 6967261 (USA).

17. Sanju F, Manmohan K, Lalit V Radiation synthesis of superbasorbent poly(acrylic acid)-carrageenan hydrogels. Radiation Physio and Chemistry. 2004. (69):481-6.

18. Tomar RS, Indu G, Reena S, Nagpal AK. Synthesis of poly(acrylamide-co-acrylic acid)-based superabsorbent hydrogels by gamma radiation: study of swelling behaviour and network parameters. Designed Monomers and Polymers. 2007. 10(1):49-66.
Published
2010-04-30
How to Cite
DJAJANEGARA, IRA; WAHYUDI, PRIYO. Uji Sitotoksisitas Ekstrak Etanol Herba Ceplukan (Physalis angulata Linn.) terhadap Sel T47D secara In Vitro. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 8, n. 1, p. 41-47, apr. 2010. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/365>. Date accessed: 05 nov. 2024.
Citation Formats
Issue
Vol 8 No 1 (2010): JIFI
Section
Articles
License

Licencing
All articles in Jurnal Ilmu Kefarmasian Indonesia are an open-access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which permits unrestricted non-commercial used, distribution and reproduction in any medium.

This licence applies to Author(s) and Public Reader means that the users mays :

  • SHARE:
    copy and redistribute the article in any medium or format
  • ADAPT:
    remix, transform, and build upon the article (eg.: to produce a new research work and, possibly, a new publication)
  • ALIKE:
    If you remix, transform, or build upon the article, you must distribute your contributions under the same license as the original.
  • NO ADDITIONAL RESTRICTIONS:
    You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

It does however mean that when you use it you must:

  • ATTRIBUTION: You must give appropriate credit to both the Author(s) and the journal, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.

You may not:

  • NONCOMMERCIAL: You may not use the article for commercial purposes.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.