Pengaruh Lupene-ol dan Lupene-on dari Aegle marmelos Correa terhadap Pelepasan Enzim β-hexoaminidase dari Sel Mast

  • AGUNG ENDRO NUGROHO UNIVERSITAS GADJAH MADA
  • SUGENG RIYANTO UNIVERSITAS GADJAH MADA
  • MOHAMAD ASPOLLAH SUKARI UNIVERSITI PUTRA MALAYSIA
  • KAZUTAKA MAEYAMA UNIVERSITY GRADUATE SCHOOL OF MEDICINE SHITSUGAWA

Abstract

20(29)-lupene-3α-ol and 20(29)-lupene-3-on are pentacyclic triterpenes isolated from Aegle marmelos Correa collected in Yogyakarta, Indonesia. Their molecular structures were confirmed in Universiti Putra Malaysia. These compounds were obtained from petroleum ether extract of the plant’s stem bark. This study investigated the effects of the compounds on the β -hexoaminidase enzyme release from mast cell culture (RBL-2H3 cell line). DNP24-BSA was used as immunologic inducer for the β -hexoaminidase release from the mast cells. The enzyme release was determined by colorimetric methods with a substrate, p-nytrophenyl-2-acetamydo-2-deoxy- β -D-gluko-pyranocide, and a microplate reader at 405 nm. lt was found that treatment with 20 µg/ml DNP24-B SA could stimulate the release of β -hexoaminidase from RBL-ZH3 cells by 3O.83 ± 1.10%. Lupene-ol and lupene-on showed inhibitory effects on the β -hexoaminidase release from RBL-2H3 cells induced by DNP24-BSA in dose-dependent manner. At the dose of 100 µM, lupene-ol and lupene-on inhibited  the β -hexoaminidaserelease by 35.69i4.7 5% and 39.19 9.56%, respectively. The ICN values of their effects on DNP24-BSA experiments were 59.40 µM and 72.51 µM. The results suggested that the inhibitory effect of lupene-ol and lupene-on on the β-hexoaminidase release involved mechanisms related to the interaction of IgE on the RBL-2H3 cells surface or intracellular signal transductions found in the mast cell degranulation. Key words: Aegle marmelos Correa, lupene-ol, lupene-on, mast cells, β -hexoaminidase enzyme.

References

1. Casal JA, Cano E, Tutor JC. Beta-hexosaminidase isoenzyme profiles in serum, plasma, platelets and
mononuclear, polymorphonuclear and unfractionated total leukocytes. Clin Biochem, 2005.3 8(10):93 8-42.


2. Mahuran DJ. B-hexosaminidase: biosynthesis and processing of the normal enzyme, and identification of mutations causing Jewish Tay-Sachs disease. Clin Biochem. 1995.28(2); 101-6.


3. Marinkovic DV and Marinkovic JN. Purification of two hexosaminidases from human kidney. Biochem J. 1977.163:133-40.

4. Schwartz LB, Austen KF, Wasserman SI. Immunologic release of beta-hexosaminidase and beta-glucuronidase from purified rat serosal mast cells. J Immunol. 1979.123(4):1445-50.

5. Lampronti I, Martello D, Bianchi N, Borgatti M, Lambertini E, Piva R, Jabbar S, Choudhuri MS, Khan MT, Gambari R. In vitro antiproliferative effects on human tumor cell lines of extracts from the Bangladeshi medicinalplant Aegle marmelos Correa. Phytomedicine. 2003.10(-4); 300-8.

6. Arul V, Miyazaki S, Dhananjayan R. Studies on the antiinflammatory, antipyretic, and analgesic properties of the leaves of Aegle marmelos Corr. J Ethnopharmacol. 2005.96(1-2):159-63. `

7. Upadhya S, Shanbhag KK, Suneetha G, Balachandra NM, Upadhya S. A study of hypoglycemic and antioxidant activity of Aegle marmelos in alloxan induced diabetic rats. Indian J Physiol Pharmacol. 2004.48(4):476-80.

8. Rana BK, Singh UP, Taneja V. Antifungal activity and kinetics of inhibition by essential oil isolated from leaves of Aegle marmelos. J Ethnopharmacol. 1997.57(1):29-34.

9. Riyanto S. Phytochemical studies and bioactivity tests of Murraya paniculata Jack, Aegle marmelos Correa, and Zingiber amaricans Blume [dissertation] Selangor: Universiti Putra Malaysia; 2003.

10. Sunitha S, Nagaraj M, Varalakshmi P. I-Iepatoprotective effect of lupeol and lupeol linoleate on tissue antioxidant defence system in cadmium-induced hepatotoxicity in rats. Fitoterapia. 2001.72(5):516- 23.

11. Lima EM, Medeiros JM, Davin LB. Pentacyclic triterpenes from Euphorbia stygiana. Phytochemistry. 2003.63(4):421-5.

12. Chumkaew P, Kato S, Chantrapromma K. A new triterpenoid ester from the fruits of Bruguiera parviflora. Chem Pharm Bull. 2005.53(1):95-6.

13. Gutierrez-Lugo MT, Deschamps JD, Holman TR, Suarez E, Timmermann BN. Lipoxygenase inhibition by anadanthoflavone, a new flavonoid from the aerial parts of Anadenanthem colubrina. Planta Med. 2004.70(3):263-5.

14. Kuo YH, Chang CI, Li SY, Chou CJ, Chen CF, Kuo YH, Lee KH. Cytotoxic constituents from the stems of Diospyros maritima. Planta Med. 1997.63(4):363-5.

15. Suksamrarn A, Tanachatchairatana T, Kanokmedhakul S. Antiplasmodial triterpenes from twigs of Gardenia saxatilis. J Ethnopharmacol. 2003.88(2-3):275-57.

16. Geetha T, Varalakshmi P. Anti-inflammatory activity of lupeol and lupeol linoleate in rats. J Ethnopharmacol. 2001.76(1):77-80.

17. Vasconcelos JF, Teixeira MM, Barbosa-Filho JM, Lucio AS, Almeida JR, de Queiroz LP, Ribeiro-Dos-Santos R, Soares MB. The triterpenoid lupeol attenuates allergic airway inflammation in a murine model. Int Immunopharmacol. 2008. 8(9):1216-21.

18. Bottcher I, Ulrich M, Hirayama N, Ovary Z. Production of monoclonal mouse IgE antibodies with DNP specificity by hybrid cell lines. Int Arch Allergy Appl Immunol. 1980.61(2):248-50.

19. Liu FT, Bohn JW, Ferry EL, Yamamoto H, Molinaro CA, Sherman LA, Klimnan NR, Katz DH. Monoclonal dinitrophenyl-specific murine IgE antibody: preparation, isolation, and characterization. J Immunol. 1980.124(6): 2728-37.

20. Orida N, Feldman JD, Katz DH, Liu FT. IgE-mediated chemotaxis of rat basophilic leukemia cells towards specific antigen. J Exp Med. 1983. 157(6):2166-71.

21. Dale MM, Foreman JC, Fan TD. Textbook of Immunopharmacology. rd ed. Oxford: Blackwell Scientific Publication: p.21-34.

22. Metcalfe DD, Baram D, Mekori YA. Mast cells. Physiol Rev. 1997.77:1033-64.


23. Ikawati Z, Wahyuono S, Maeyama K. Screening of several Indonesian medicinal plants for their inhibitory effect on histamine release from RBL-2H3 cells. J Ethnopharmacol. 2001.75(2-3); 249-56.

24. Subowo. Imunologi klinik. Bandung: Angkasa; 1993. hal.9-35.

25. Bani S, Kaul A, Khan B, Ahmad SF, Suri KA, Gupta BD, Satti NK, Qazi GN. Suppression of Tlymphocyte activity by lupeol isolated from Crataeva religiosa. Phytother Res. 2006.20(4):279-87.

26. Lapenna D, Ciofani G, Pierdomenico SD, Giamberardino MA, Cuccurullo F. Dihydrolipoic acid inhibits 15-lipoxygenase-dependent lipid peroxidation. Free Radic Biol Med. 2003.35(10):1203-9.

27. Fernandez MA, de las Heras B, Garcia MD, Saenz MT, Villar A. New insights into the mechanism of action of the anti-inflammatory triterpene lupeol. J Pharm Pharmacol. 2001.53(11):1533-9.

28. Ramirez Apan AA, Perez-Castorena AL, de Vivar AR. Anti-inflammatory constituents of Mortonia greggii Gray. Z Naturforsch. 2004. 59(3-4):237-43.

29. Lima LM, Perazzo FF, Tavares CJC, Bastos JK. Anti-inflammatory and analgesic activities of the ethanolic extracts from Zanthoxylum riedelianum (Rutaceae) leaves and stem bark. J Pharm Pharmacol. 2007.59(8):1151-8.

30. Nguemfo EL, Dimo T, Dongmo AB, Azebaze AG, Alaoui K, Asongalem AE, Cherrah K Kamtchouing P. Anti-oxidative and anti-inflammatory activities of some isolated constituents from the stem bark of Allanblackia moniicola Staner L.C (Guttiferae). Inflammopharmacology. 2009. 17(1):37-41.
Published
2010-04-30
How to Cite
NUGROHO, AGUNG ENDRO et al. Pengaruh Lupene-ol dan Lupene-on dari Aegle marmelos Correa terhadap Pelepasan Enzim β-hexoaminidase dari Sel Mast. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 8, n. 1, p. 55-60, apr. 2010. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/370>. Date accessed: 22 dec. 2024.
Citation Formats
Issue
Vol 8 No 1 (2010): JIFI
Section
Articles
License

Licencing
All articles in Jurnal Ilmu Kefarmasian Indonesia are an open-access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which permits unrestricted non-commercial used, distribution and reproduction in any medium.

This licence applies to Author(s) and Public Reader means that the users mays :

  • SHARE:
    copy and redistribute the article in any medium or format
  • ADAPT:
    remix, transform, and build upon the article (eg.: to produce a new research work and, possibly, a new publication)
  • ALIKE:
    If you remix, transform, or build upon the article, you must distribute your contributions under the same license as the original.
  • NO ADDITIONAL RESTRICTIONS:
    You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

It does however mean that when you use it you must:

  • ATTRIBUTION: You must give appropriate credit to both the Author(s) and the journal, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.

You may not:

  • NONCOMMERCIAL: You may not use the article for commercial purposes.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.