Ekstraksi dan Identifikasi Senyawa Antimikroba Herba Meniran (Phyllanthus niruri L.)
Abstract
Phyllanthus niruri L. herbs was extracted using ethanol 96%, ethylacetate and n-hexane. The rendements were respectively 49.25% (for ethanol extract), 4.18% (ethylacetate extract) and0.78% (n-hexane extract). Identification of the ethanol extract using phytochemical assays resulted in alkaloid, flavonoid, saponin, and tannin compounds. Purification of the ethanol extract using thin layer chromatography (TLC) with n-hexane-ethylacetate (6:4) followed by the inhibitory activity test using bioautography TLC showed that the spot of Rf 0,46 inhibited Staphylococcus aureus and that of Rf 0,66 inhibited Candida albicans. Analysis with infra red spectrophotometer showed that the ethanol extract has hydroxyl (-OH) and carbonyl (C=O) functional groups.
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7. Elfahmi, Batterman S, Koulman A, Hackl T, Bos R, Kayser O, Woerdenbag HJ, Quax WJ. Lignans from cell suspension culture of Phyllanthus niruri, an Indonesian medicinal plant. Journal of Natural Products. 2006. 69:55-8.
8. Harborne JB. Phytochemical methods. A guide to modern techniques of plants analysis. 3rd ed. London: Chapman & Hall; 1998. p. 302.
9. Hertiani T, Palupi SI, Sanliferianti, Nuiwindasari DH. In vitro test on antimicrobial potency against Staphylococcus aureus, Escherichia coli, Shigella dysentriae and Candida albicans of some herbs tradisionally used cure infection diseases. Pharmacon. 2003.4(2): 89-95.
10. Priyono RE. Skrining golongan senyawa kimia dan uji antibakteri ekstrak etanol 50% dan n-heksan biji kedawung (Parkia timoriana Merr.), daun inggu (Ruta angustyfolia L.), dan kulit kayu rapat (Parameria barbata Schum.) terhadap bakteri Gram positif [tesis]. Depok: Biologi FMIPA UI; 2004. hal. 68.
11. Indrayani L, Soetjipto H, Sihasale L. Skrining fitokimia dan uji toksisitas ekstrak daun pecut kuda (Stachytarphetajamaicensis L.Vahl) terhadap larva udang Artemia salina Leach. Berkala Penelitian Hayati. 2006.12:57-61.
12. Ismaini L. Studi aktivitas dan analisis kimia senyawa antibakteri dari ekstrak biji picung (Pangium edule Reinw.) [tesis]. Depok: Biologi FMIPA UI; 2007. hal. 87.
13. Usia T, Banskota AH, Tezuka Y, Midorikawa K, Matsushige K, Kadota- S. Chemical constituents of Chinese propolis and their antiproliferative activities. Journal Natural Products. 2002. 65(5): 673-76.
14. Direktorat Jendral Pengawasan Obat dan Makanan. Farmakope Indonesia IV. Jakarta: Departemen Kesehatan R.I; 1995. hal. 1290.
15. Badan POM. Monograii ekstrak tumbuhan obat Indonesia. Vol 1. Jakarta: Badan POM; 2004. p. 67-70.
16. Farnsworth. Biological and phytochemical screening of plants. Journal Pharmaceutical Science. 1966. 55(3): 243-269.
17. Stahl E. Analisis obat secara kromatografi dan mikroskopi. Terjemahan dari Drug analisis by chromathography and microscopy: a practical suplement to pharmacopoias, oleh Padmawinata, Sudiro KI. Bandung: ITB; 1985. hal. 267.
18. Anonim. Materi Medika Indonesia III. Jakarta: Departemen Kesehatan Republik Indonesia; 1977. hal. 523-28.
19. Mdlolo CM, Shandu JS, Oyedeji OA. Phytochemical constituents and antimicrobial studies of two South Africa Phyllanthus species. African Journal of Biotechnology. 2008.7(5): 639-43.
20. Sylvia S, Soetarno, Yulinah E. Telaah fitokimia ekstrak etanol buah cabe dan uji aktivitasnya sebagai antimikroba. Bandung: Sekolah Farmasi ITB; 1996.
21. Sudirman LI. Detection of antimicrobial compounds isolated from several tropical Lentinus by bioautographic method. Hayati Journal of Biosciences. 2005.12(2): 16-22.
22. Jawetz E. Prinsip kerja obat antimikroba. 3rd ed. Dalam Katzung B.G. Fannakologi dasar dan klinik. Jakarta: Kedokteran EGC; 1992. hal. 607-12.
23. Silverstein RM, Bassler GC, Morrill TC. Spectrometric identification of organic compounds.4th ed. John Wiley & Sons, New York; 1963. 95-105.
Published
2009-09-30
How to Cite
MANGUNWARDOYO, WIBOWO; CAHYANINGSIH, ENI; USIA, TEPY.
Ekstraksi dan Identifikasi Senyawa Antimikroba Herba Meniran (Phyllanthus niruri L.).
JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 7, n. 2, p. 57-63, sep. 2009.
ISSN 2614-6495.
Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/372>. Date accessed: 23 nov. 2024.
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