Ekstraksi dan Identifikasi Senyawa Antimikroba Herba Meniran (Phyllanthus niruri L.)
Abstract
Phyllanthus niruri L. herbs was extracted using ethanol 96%, ethylacetate and n-hexane. The rendements were respectively 49.25% (for ethanol extract), 4.18% (ethylacetate extract) and0.78% (n-hexane extract). Identification of the ethanol extract using phytochemical assays resulted in alkaloid, flavonoid, saponin, and tannin compounds. Purification of the ethanol extract using thin layer chromatography (TLC) with n-hexane-ethylacetate (6:4) followed by the inhibitory activity test using bioautography TLC showed that the spot of Rf 0,46 inhibited Staphylococcus aureus and that of Rf 0,66 inhibited Candida albicans. Analysis with infra red spectrophotometer showed that the ethanol extract has hydroxyl (-OH) and carbonyl (C=O) functional groups.
References
1. Heyne K. Tumbuhan berguna Indonesia II. Terjemahan dari De nuttige planten van Indonesie, oleh Badan Litbang Kehutanan. Jakarta: Yayasan Sarana Wana Jaya; 1987. hal. 2521.
2. Venkateswaran PS, Millman I, Blumberg BS. Effects of an extract from Phyllanthus niruri on hepatitis B and woodchuck hepatitis viruses: in vitro and in vivo studies. Proceeding National Academic Science, USA.1987.(84): 274-78. ,
3. Naik AD, Juvekar AD. Effect of alkaloid extract of Phyllanthus niruri on HIV replication. Indian Journal Medicinal Science. 2003.57:387-93.
4. Kardinan A, Kusuma FR. Meniran penambah daya tahan tubuh alami. Jakarta: Agromedia Pustaka; 2004. hal. 61.
5. Badan POM. Meniran (Phyllanthus niruri L). J akarta: Badan POM; 2006. hal. 13.
6. Asean Countries National Agency of Drug and Food Control. Standard of Asean herbal medicines. Vol. II. Jakarta: Asean countries, National Agency of Drug and Food Control; 2004. p. 202.
7. Elfahmi, Batterman S, Koulman A, Hackl T, Bos R, Kayser O, Woerdenbag HJ, Quax WJ. Lignans from cell suspension culture of Phyllanthus niruri, an Indonesian medicinal plant. Journal of Natural Products. 2006. 69:55-8.
8. Harborne JB. Phytochemical methods. A guide to modern techniques of plants analysis. 3rd ed. London: Chapman & Hall; 1998. p. 302.
9. Hertiani T, Palupi SI, Sanliferianti, Nuiwindasari DH. In vitro test on antimicrobial potency against Staphylococcus aureus, Escherichia coli, Shigella dysentriae and Candida albicans of some herbs tradisionally used cure infection diseases. Pharmacon. 2003.4(2): 89-95.
10. Priyono RE. Skrining golongan senyawa kimia dan uji antibakteri ekstrak etanol 50% dan n-heksan biji kedawung (Parkia timoriana Merr.), daun inggu (Ruta angustyfolia L.), dan kulit kayu rapat (Parameria barbata Schum.) terhadap bakteri Gram positif [tesis]. Depok: Biologi FMIPA UI; 2004. hal. 68.
11. Indrayani L, Soetjipto H, Sihasale L. Skrining fitokimia dan uji toksisitas ekstrak daun pecut kuda (Stachytarphetajamaicensis L.Vahl) terhadap larva udang Artemia salina Leach. Berkala Penelitian Hayati. 2006.12:57-61.
12. Ismaini L. Studi aktivitas dan analisis kimia senyawa antibakteri dari ekstrak biji picung (Pangium edule Reinw.) [tesis]. Depok: Biologi FMIPA UI; 2007. hal. 87.
13. Usia T, Banskota AH, Tezuka Y, Midorikawa K, Matsushige K, Kadota- S. Chemical constituents of Chinese propolis and their antiproliferative activities. Journal Natural Products. 2002. 65(5): 673-76.
14. Direktorat Jendral Pengawasan Obat dan Makanan. Farmakope Indonesia IV. Jakarta: Departemen Kesehatan R.I; 1995. hal. 1290.
15. Badan POM. Monograii ekstrak tumbuhan obat Indonesia. Vol 1. Jakarta: Badan POM; 2004. p. 67-70.
16. Farnsworth. Biological and phytochemical screening of plants. Journal Pharmaceutical Science. 1966. 55(3): 243-269.
17. Stahl E. Analisis obat secara kromatografi dan mikroskopi. Terjemahan dari Drug analisis by chromathography and microscopy: a practical suplement to pharmacopoias, oleh Padmawinata, Sudiro KI. Bandung: ITB; 1985. hal. 267.
18. Anonim. Materi Medika Indonesia III. Jakarta: Departemen Kesehatan Republik Indonesia; 1977. hal. 523-28.
19. Mdlolo CM, Shandu JS, Oyedeji OA. Phytochemical constituents and antimicrobial studies of two South Africa Phyllanthus species. African Journal of Biotechnology. 2008.7(5): 639-43.
20. Sylvia S, Soetarno, Yulinah E. Telaah fitokimia ekstrak etanol buah cabe dan uji aktivitasnya sebagai antimikroba. Bandung: Sekolah Farmasi ITB; 1996.
21. Sudirman LI. Detection of antimicrobial compounds isolated from several tropical Lentinus by bioautographic method. Hayati Journal of Biosciences. 2005.12(2): 16-22.
22. Jawetz E. Prinsip kerja obat antimikroba. 3rd ed. Dalam Katzung B.G. Fannakologi dasar dan klinik. Jakarta: Kedokteran EGC; 1992. hal. 607-12.
23. Silverstein RM, Bassler GC, Morrill TC. Spectrometric identification of organic compounds.4th ed. John Wiley & Sons, New York; 1963. 95-105.
2. Venkateswaran PS, Millman I, Blumberg BS. Effects of an extract from Phyllanthus niruri on hepatitis B and woodchuck hepatitis viruses: in vitro and in vivo studies. Proceeding National Academic Science, USA.1987.(84): 274-78. ,
3. Naik AD, Juvekar AD. Effect of alkaloid extract of Phyllanthus niruri on HIV replication. Indian Journal Medicinal Science. 2003.57:387-93.
4. Kardinan A, Kusuma FR. Meniran penambah daya tahan tubuh alami. Jakarta: Agromedia Pustaka; 2004. hal. 61.
5. Badan POM. Meniran (Phyllanthus niruri L). J akarta: Badan POM; 2006. hal. 13.
6. Asean Countries National Agency of Drug and Food Control. Standard of Asean herbal medicines. Vol. II. Jakarta: Asean countries, National Agency of Drug and Food Control; 2004. p. 202.
7. Elfahmi, Batterman S, Koulman A, Hackl T, Bos R, Kayser O, Woerdenbag HJ, Quax WJ. Lignans from cell suspension culture of Phyllanthus niruri, an Indonesian medicinal plant. Journal of Natural Products. 2006. 69:55-8.
8. Harborne JB. Phytochemical methods. A guide to modern techniques of plants analysis. 3rd ed. London: Chapman & Hall; 1998. p. 302.
9. Hertiani T, Palupi SI, Sanliferianti, Nuiwindasari DH. In vitro test on antimicrobial potency against Staphylococcus aureus, Escherichia coli, Shigella dysentriae and Candida albicans of some herbs tradisionally used cure infection diseases. Pharmacon. 2003.4(2): 89-95.
10. Priyono RE. Skrining golongan senyawa kimia dan uji antibakteri ekstrak etanol 50% dan n-heksan biji kedawung (Parkia timoriana Merr.), daun inggu (Ruta angustyfolia L.), dan kulit kayu rapat (Parameria barbata Schum.) terhadap bakteri Gram positif [tesis]. Depok: Biologi FMIPA UI; 2004. hal. 68.
11. Indrayani L, Soetjipto H, Sihasale L. Skrining fitokimia dan uji toksisitas ekstrak daun pecut kuda (Stachytarphetajamaicensis L.Vahl) terhadap larva udang Artemia salina Leach. Berkala Penelitian Hayati. 2006.12:57-61.
12. Ismaini L. Studi aktivitas dan analisis kimia senyawa antibakteri dari ekstrak biji picung (Pangium edule Reinw.) [tesis]. Depok: Biologi FMIPA UI; 2007. hal. 87.
13. Usia T, Banskota AH, Tezuka Y, Midorikawa K, Matsushige K, Kadota- S. Chemical constituents of Chinese propolis and their antiproliferative activities. Journal Natural Products. 2002. 65(5): 673-76.
14. Direktorat Jendral Pengawasan Obat dan Makanan. Farmakope Indonesia IV. Jakarta: Departemen Kesehatan R.I; 1995. hal. 1290.
15. Badan POM. Monograii ekstrak tumbuhan obat Indonesia. Vol 1. Jakarta: Badan POM; 2004. p. 67-70.
16. Farnsworth. Biological and phytochemical screening of plants. Journal Pharmaceutical Science. 1966. 55(3): 243-269.
17. Stahl E. Analisis obat secara kromatografi dan mikroskopi. Terjemahan dari Drug analisis by chromathography and microscopy: a practical suplement to pharmacopoias, oleh Padmawinata, Sudiro KI. Bandung: ITB; 1985. hal. 267.
18. Anonim. Materi Medika Indonesia III. Jakarta: Departemen Kesehatan Republik Indonesia; 1977. hal. 523-28.
19. Mdlolo CM, Shandu JS, Oyedeji OA. Phytochemical constituents and antimicrobial studies of two South Africa Phyllanthus species. African Journal of Biotechnology. 2008.7(5): 639-43.
20. Sylvia S, Soetarno, Yulinah E. Telaah fitokimia ekstrak etanol buah cabe dan uji aktivitasnya sebagai antimikroba. Bandung: Sekolah Farmasi ITB; 1996.
21. Sudirman LI. Detection of antimicrobial compounds isolated from several tropical Lentinus by bioautographic method. Hayati Journal of Biosciences. 2005.12(2): 16-22.
22. Jawetz E. Prinsip kerja obat antimikroba. 3rd ed. Dalam Katzung B.G. Fannakologi dasar dan klinik. Jakarta: Kedokteran EGC; 1992. hal. 607-12.
23. Silverstein RM, Bassler GC, Morrill TC. Spectrometric identification of organic compounds.4th ed. John Wiley & Sons, New York; 1963. 95-105.
Published
2009-09-30
How to Cite
MANGUNWARDOYO, WIBOWO; CAHYANINGSIH, ENI; USIA, TEPY.
Ekstraksi dan Identifikasi Senyawa Antimikroba Herba Meniran (Phyllanthus niruri L.).
JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 7, n. 2, p. 57-63, sep. 2009.
ISSN 2614-6495.
Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/372>. Date accessed: 27 july 2024.
Issue
Section
Articles
License
Licencing
All articles in Jurnal Ilmu Kefarmasian Indonesia are an open-access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which permits unrestricted non-commercial used, distribution and reproduction in any medium.
This licence applies to Author(s) and Public Reader means that the users mays :
- SHARE:
copy and redistribute the article in any medium or format - ADAPT:
remix, transform, and build upon the article (eg.: to produce a new research work and, possibly, a new publication) - ALIKE:
If you remix, transform, or build upon the article, you must distribute your contributions under the same license as the original. - NO ADDITIONAL RESTRICTIONS:
You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
It does however mean that when you use it you must:
- ATTRIBUTION: You must give appropriate credit to both the Author(s) and the journal, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
You may not:
- NONCOMMERCIAL: You may not use the article for commercial purposes.
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Tools
