Karakterisasi Senyawa Utama Ekstrak Daun Aglaia elliptica Blume dan Uji Sitotoksisitas pada Sel Kanker Payudara

  • AGUNG ERU WIBOWO BADAN PENGKAJIAN DAN PENERAPAN TEKNOLOGI
  • FRANS SUYATNA UNIVERSITAS INDONESIA
  • WAHONO SUMARYONO BADAN PENGKAJIAN DAN PENERAPAN TEKNOLOGI
  • NURYATI CHAERANI SIREGAR UNIVERSITAS INDONESIA

Abstract

AIglaia elliptica Blume is one of plant species from the genus of Aglaia, belonging to the family of Meliaceae. This group of plants is known to have powerful cytotoxic effect. This research was conducted to isolate and characterize major compound(s) from ethanol extract of Aglaia elliptical leaf that can be used as marker compound(s) for extract standardization. Approximately 1000 g of Aglaia elliptica Blume leaf dry powder was extracted with ethanol using maceration method. Ethanol extract was then fractionated using n-hexane and ethyl acetate. Six sub-fractions were obtained from the Vacuum Liquid Chromatography (VLC) process of ethyl acetate fraction. Based on the HPLC profile and separation process using column chromatography and preparative HPLC, a major compound (with the weight of 36.80 mg) was successfully isolated from sub-fraction H. Analyses on its UV absorbance pattern, mass spectra, and proton NMR identified the isolated compound as a compound with molecular weight of 300, C18H24N2O2, known as odorin. Cytotoxicity test showed that odorin has inhibition effect against MCP-7 cell line with IC5O of 153.32 ppm.

References

1. Kim S, Salim AA, Swanson SM, and Kinghom A.Potential of cyclopenta[b]benzofurans from Aglaia species in cancer chemotherapy. Anti-Cancer Agents in Medicinal Chemistry. 2006.(6): 319-45.

2. Chaidir. Insektizide rocaglamind-derivate und verwandte verbindungen aus Aglaia-arten (Meliaceae) [Dissertation zur Erlangung desnattuwissenchaftlichen Doktogrades der bayerischen Julius-MaXimillana]. Wuerzburg: Universitat Wuerzburg; 2002.

3. Bohnenstengel F. lnhaltsstoffe Austrophischen Meliaceen der gattungan Aglaia und Melia, isoleurung, sturkturauffklarung und evaluierung der pharmakologisschen aktivitatenm [Dissertation zur Erlangung des naturwissenchaftlichen Doldogrades der bayerischen Julius-Maximillana]. Wuerzburg: Universitat Wuerzburg; 2000.
4. Nugroho BW, Gussregen B, Wray V, Witte L, Bringmann G, and Proksch P. Insectidal rocaglamide derivates from Aglaia elliptica and Aglaia harmsiana. Phytochemistry. 1997.45(8)1579-85.

5. MTT cell proliferation assay instruction catalog number 30-l0l0l<, ATCC [serial online] 2001. Diambil dari http//www.atcc.org. Diakses tanggal 25 juni 2006.

6. Inada A, Sorano T, Murata H, Inatomi XC Damaedi D, and Nakanishi T. Diamide derivatives and cycloartanes from the leaves of Aglaia elliptical. Chem. Pharm. Bull. 2001.49(9):1226-28.

7. Abraham R.J, Fisher J, and Loftus P. Introduction to NMR spectroscopy. New York: John Wiley & Sons Ltd; 1991.

8. Sanders JKM, Hunter BK. Modem NMR spectroscopy. A Guide for Chemist. Oxford: Oxford University Press; 1987.
Published
2009-09-30
How to Cite
WIBOWO, AGUNG ERU et al. Karakterisasi Senyawa Utama Ekstrak Daun Aglaia elliptica Blume dan Uji Sitotoksisitas pada Sel Kanker Payudara. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 7, n. 2, p. 73-78, sep. 2009. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/376>. Date accessed: 23 june 2024.
Section
Articles