Pemisahan Delapan Vitamin Larut Air secara Kromatografi Pasangan Ion

  • NOVI YANTIH UNIVERSITAS PANCASILA

Abstract

Ion-pair chromatography is applicable for separating mixtures of acids and bases, either in ionic or nonionic form, such as mixture of ascorbic acid, thiamine hydrochloride, riboilavin, nicotinamide, pyridoxine hydrochloride, folic acid, and cyanocobalamine, and menadion sodium bisulphite. Separation using ion-pair chromatography is highly influenced by the type and concentration of the counter ion and the selected pH of mobile phase. Eight Water soluble vitamins Were separated in a single analysis Withtotal analysis time never exceeded 12 minutes by using a reversed-phase column (CS) with a mixture of methanol and solution of 5 mM sodium hexanesulphonate at pH 3.50 (49: 151) as mobile phase, at a flow rate of l ml per minute, and ultraviolet detector at 275 nm. Satisfactory separation was shown by the resolution values of more than 1.5, which were between 3.8-10.6.

References

1. Ibrahim S. Pengembangan metode analisis menggunakan kromatografi cair kinerja tinggi. Seminar on HPLC Application for Analysis of Drugs, Food, and Environment. Bandung: Penerbit ITB; 1998. hal. 2-8.

2. Willard H, Merritt HLL Jr, Dean JA, and Settle FA Jr. Instrumental methods of analysis. 7th ed. Belmont: Wadsworth Publ. Co.; 1988. p. 580-8, 615-33.

3. Meyer RV. Practical high performance liquid chromatography. Guildford: John Wiley & Sons Ltd.; 1978.p. 1,15-41, 169-174.

4. Yantih N, Amir MM, Slamet I, Rahmana E. Determination of seven water soluble vitamins in tablet simultaneously by reversed-phase high performance liquid chromatography. International Seminar on Pharmaceutics: up date on pharmaceutical innovation and new drug delivery systems, School of Phannacy ITB, Bandung 31 Oktober - 1 November 2007.

5. Amidzic R, Brboric J, Cudina O, and Viadimirov S. RP-HPLC Determination of vitamin Bl, B3, B6, folic acid, and B12 in multivitamin tablets. J. Serb. Chem. Soc. 2005.70(10):1229-135.

6. Heudi O, Tamara K, and Patric F. Separation of Water- soluble vitamins by reversed-phase high performance liquid chromatography with ultra-violet detection: application to polyvitaminated premixes. J. Chrom. 2005. 1070(1-2):49-56.

7. Albala HS, Veciana NMT, Izquierdo PM, Marine FA. Determination of Water-soluble vitamins in infant milk by high-performance liquid chromatography. Scientific Meeting ofthe Group of Chromatography and Related Techniques ofthe Spanish Royal Society of Chemistry No. 25, Barcelona. 778(1-2): 247-53.
Published
2009-09-30
How to Cite
YANTIH, NOVI. Pemisahan Delapan Vitamin Larut Air secara Kromatografi Pasangan Ion. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 7, n. 2, p. 85-90, sep. 2009. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/379>. Date accessed: 23 nov. 2024.
Section
Articles