Pemakaian Sel HeLa dalam Uji Sitotoksisitas Fraksi Kloroform dan Etanol Ekstrak Daun Annona squamosa

  • IRA DJAJANEGARA BADAN PENGKAJIAN dan PENERAPAN TEKNOLOGI
  • PRIO WAHYUDI BADAN PENGKAJIAN dan PENERAPAN TEKNOLOGI

Abstract

Custard apple (Annona squamosa) is a favorite fruit in Indonesia. The leave of this plant is used and known for long time as an astringent, antiinflammatory, birth control, and antitumor agents. Previous experiments on toxicity using brine shrimp lethality test (BSLT) method indicated that the chloroform fraction of custard apple leaves was toxic to the shrimp larvae with LC50 value at 1.77µg/ml. This experiment was carried out to investigate further the possible role of custard apple leaves as an anticancer agent using HeLa cell line. Experiment was initiated by extracting the leaves using 70% ethanol by means of maceration. The macerate was then fractionated further using chloroform as solvent. The ethanol fraction and chloroform fraction were used in cytotoxicity assay using (cervix cancer) HeLa cell line. Cytotoxicity assay Was done using direct counting method. The amount of living cells were observed and counted, death percentage Was then determined and probit analysis Was applied to determine the LC50 value. LC50 value for ethanol fraction from the leaves of custard apple (Annona squamosa) Was 7.695 µg/ml While for chloroform fraction was 4.547 µg/ml based on cytotoxicity analysis using HeLa cells. This experiment concluded that theleaves of custard apple (Annona squamosa) has cytotoxicity toward HeLa cell lines which confirmed the previous toxicity experiment using brine shrimp lethality test (BSLT) method. Overall, this experiment indicated that the leaves of custard apple (Annona squamosa) have anticancer activity.

References

1. Ratna. Apa yang harus anda ketahui tentang kanker (artikel online). Diambil dari: http://www.lndosiar. co.id/infomedis. Diakses tanggal 9 Desember 2005 .

2. Eckholom P. Masalah kesehatan. Jakarta: Yayasan Obor Indonesia; 1982. hal. 67-8.

3. Urivi, V. Menu untuk penderita kanker. Jakarta: Puspa swara; 2002. hal. 3, 30-2.

4. Sastomidj ojo S. Obat asli Indonesia. Jakarta: Pustaka Rakyat; 1997. hal. 6-9.

5. Sudarsono. Tumbuhan obat II. Yogyakarta: Pusat Studi Obat Tradisional Universitas Gajah Mada; 2002. hal. 9-12.

6. Dalimaita S. Atlas tumbuhan obat Indonesia. Jilid II. Jakarta: Puspa Swarag 2003. hal. 45-7.

7. Wahyono S, Rachman A. Uji toksisitas beberapa tumbuhan obat Indonesia dengan Brine Shrimp Lethaliy Test (BSLT). Dalam: Majalah Farmasi Indonesia. 1995. 108-44.

8. Departemen Kesehatan RI. Materia Medika Indonesia Jilid V Jakarta: Departemen Kesehatan RI; 1989. hal. 523:55.

9. Harborne. Metode fitokimia. Terbitan kedua. Terjemahan dari: Phytochemical methods. Oleh: Padmawinata, Kosasih, Iwan S. Bandungf ITB ; 1996. hal. 6, 147.

10. Departemen Kesehatan RI. Sediaan Galenik. Jakarta: Dirjen POM Departemen Kesehatan RI; 1986. hal. 10-2.

11. Departemen Kesehatan Rl. Materia Medika Indonesia Jilid II. Jakarta: Departemen Kesehatan RI; 1995. hal. 155.

12. Freshney RI. Animal cell culture, A practical approach. 1st ed. Washington DC: IRL Press; 1987. p. 3, 38, 83-8, 309412, 329-30, 336-7.

13. Doyle A, Griiifths JB. Cell and tissue culture for medical research. New York: John Wiley & Sons;2000. p. 12-6, 48-9, 409.

14. Tj ay T, Rahardja K. Obat-obat penting, penggunaan dan efek-efek sampingnya. ed. V Jakarta: Dirjen POM Depkes RI; 2002. hal. 197-8, 206-17.


15. Rosliana R, Nuryangsih S, KaryaniA. Uji sitoksisititas infus jamur kuping hitam (Auricularia polytricha). Dalam: Laporan Program Kreativitas Mahasiswa (PKM). Fakultas Farmasi Universitas Ahmad Dahlan, Yogyakarta. 2004. 22.

16. Harvey AR, Champe CP. Farmakologi ulasan bergambar. ed. IV. Jakarta: Widya Medika; 2001. hal. 94-5, 398:9.

17. Meyer BN, Ferrigni N R., Putnam JE, Jacobson LB, Nicholas DE, McLaugnin JL. Brine shrimp: A convinient general bioassay for active plant constituent, Plant. Med. 1982. 31:34-45.
Published
2009-04-30
How to Cite
DJAJANEGARA, IRA; WAHYUDI, PRIO. Pemakaian Sel HeLa dalam Uji Sitotoksisitas Fraksi Kloroform dan Etanol Ekstrak Daun Annona squamosa. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 7, n. 1, p. 7-11, apr. 2009. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/386>. Date accessed: 21 nov. 2024.
Section
Articles