Enkapsulasi Propagul Jamur Entomopatogen Beauveria bassiana Menggunakan Alginat dan Pati Jagung sebagai Produk Mikoinsektisida

  • PRIYO WAHYUDI BADAN PENGKAJIAN dan PENERAPAN TEKNOLOGI

Abstract

Negative effects of chemical pesticides experienced by farmers have encouraged researchers to develop bioinsectiscide. Beauveria bassiana is one of entomopatogen fungus that may be used as an active compound of mycoinsecticide. The main constraint of this mycoinsecticide is retaining the viability of Beauveria bassiana propagules. The encapsulation of active propagule of Beauveria bassiana using sodium alginate and corn starch was studied in this experiment. The aim is to get the formula of mycoinsecticide with a high viable propagules contain for long storage period. Liquid fermentation were proceed to produce active biomass of Beauveria bassiana, followed by encapsulation using sodium alginate (Formula I) and corn starch (Formula II). After encapsulation and the 1st, 2nd, and 3th Week of storage in room temperature, viability test was carried out. The result showed that Formula I and II were able to immobilize effectively, but the number of viable propagule was slightly reduced. The number of viable propagule of Beauveria bassiana in Formula I and ll after three Weeks storage was 8.75 x 106 cfu/g and 6.13 x 106 cfu/g respectively. It can be concluded that encapsulation using sodium alginate and corn starch could retain propagule of Beauveria bassiana in viable number.

References

1. Leathers TD. Mycopesticides: Status, challenges and potential. J. Industrial Microbiol. 1993.12:69-75.

2. Wahyudi P. Produksi insektisida berbahan aktifjamur entomopatogen Beauveria bassiana. J. Sains dan Teknologi Indonesia. Edisi Teknologi Proses. 1999. 1(9):36-42.

3. Bradley CA. Mycoinsecticide activity against Grasshoppers produced by Beauveria bassiana. United State Patent. No. 5.939.065. 1999.p.1-20.

4. Mangoendihardjo S. Teori dan praktek pengendalian biologis. Jakarta: Penerbit Universitas Indonesia UI Press; 1989. hal. 218-9.
5. Hegedus DD. Beauveria bassiana submerged conidia production in a defined medium containing chitin, two hexosamines or glucose, App. Microbiol. And Technol. 1990.(33):641-7.

6. Weiser J. Persistence of fungal insecticides: influence of environmental factors and present and future applications. Microbial and Viral pesticides. Marcel Dekker lnc. l982.p.531-55.

7. Pramono S. Potensi jamur Beauveria bassiana (Bals) Vuill terhadap Helicoverpa armigera (Hubner). Yogyakarta: Program Pasca Sarjana Universitas Gadjah Mada; 1994. hal.10-6.

8. Sudarmadji D dan Suharyanto. Deteksi keberagaman Beauveria bassiana berdasarkan pola pita protein dan uji hayati terhadap hama Helopeltis antonii. J. Mikrobiologi Tropika. 1997.(Juli):60-3.

9. Mahr S. Know your friends. Diambil dari http://www.
wisc.edu/entomology/mbcn/kyf410.html. Midwest
Biological Control News. 2000.IV(10):1-2.

10. Duvick J. Beauvericin detoxification compositions and methods. United States Patent No. 6.117.668 September 12, 2000.p.1-7.

11. Yasin M, Syamsudin, dan Baco D. Pengaruh berbagai konsentrasi cendawan Beauveria bassiana terhadap wereng jagung (Peregrinus maidis). Stigma. April 1999.VII(1):171-4.

12. Benita S. Microencapsulation methods and industrial applications. New York: Marcell Dekker Inc; 1996.p.82-3.

13. Risch, SJ. Encapsulation: Overview of uses and techniques, encapsulation and controlled release of food ingredients. Washington DC: American Chemical Society; 1995.p.2-7.

14. Feng MG, Poprawski TJ, and Khachatourians GG. Production, formulation and application of the entomopathogenic fungus Beauveria bassiana for insect control: Curent status. Biocontrol Science and Technology. 1994.p.1-25.

15. Wade A, Weller PJ (Eds.). Handbook of pharmaceutical excipients. 2nd ed. London: The Pharmaceutical Press; 1994.p.428-30, 483-8.

16. Domsch KH, Gams W, and Anderson TH. Compendium of soil fungi. Vol l. London: Academic Press; 1980.

17. Shah PA, Aebi M, and Tour U. Method to immobilize the aphid pathogenic fungus Erynia neoapbidis in an alginate matrix for biocontrol. App. and Enviro. Microbiol. 1998.64(11):-4260-3.
Published
2008-09-30
How to Cite
WAHYUDI, PRIYO. Enkapsulasi Propagul Jamur Entomopatogen Beauveria bassiana Menggunakan Alginat dan Pati Jagung sebagai Produk Mikoinsektisida. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 6, n. 2, p. 51-56, sep. 2008. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/398>. Date accessed: 21 nov. 2024.
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Articles