Sensitivitas Metode Bioautografi Kontak dan Agar Overlay dalam Penentuan Senyawa Antikapang

  • ENI KUSUMANINGTYAS BALAI BESAR PENELITIAN VETERINER
  • ESTIE ASTUTI UNIVERSITAS PANCASILA
  • DARMONO DARMONO UNIVERSITAS PANCASILA

Abstract

Bioautography method is a laboratory technique to detect substances inhibiting the growth of tested microorganisms. The sensitivity of contact and agar overlay bioautography Was compared to define a recommended method for detecting antifungal compound. In contact bioautography, n-hexane extract of Alpinia galanga was eluted with dichloromethane and toluene on a thin layer chromatography plate (silica gel GF254). The separated compounds were diffused from chromatogram spots to a Thrichophyton menzagrophytes inoculated Sabouraud Dextrose Agar plate. The plate was incubated at 37°C for 3 days. Inhibition zone was observed and the hRf value was defined. Agar overlay bioautography Was performed by covering chromatogram spots With a molten Sabouraud Dextrose Agar medium seeded by Trichophyton mentagrophytes. After solidification, the chromatogram plate was incubated at 37°C overnight and then was stained with Methyl Thiazole Tetrazolium. The inhibition growth bands were visualized and hRfs value were recorded. A contact bioautography produces oneband while agar overlay bioautography produces four bands. Agar overlay bioautography was therefore more sensitive than contact bioautography.

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Published
2008-09-30
How to Cite
KUSUMANINGTYAS, ENI; ASTUTI, ESTIE; DARMONO, DARMONO. Sensitivitas Metode Bioautografi Kontak dan Agar Overlay dalam Penentuan Senyawa Antikapang. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 6, n. 2, p. 75-80, sep. 2008. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/402>. Date accessed: 17 apr. 2024.
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Articles