Analysis of SGPT and SGOT on Rats Induced By Isoniazid to Determine Dose and Hepatoprotective Characteristic of Unripe Pineapple Juice (Ananas comosus L. Merr)
Abstract
As a consequence of Isoniazid (INH) consumption in longterm is the damage on the liver cells which is detected in high level of SGPT and SGOT in blood. Unripe pineapple fruits contained higher level of phenolic acid and flavonoids compounds than the ripe pineapple fruits, and as a result the unripe pineapple fruits juice has higher hepatoprotective potency than ripe pineapple fruits juice. The hepatoprotective activity of unripe pineapple fruits juice was determined by measuring the SGPT and SGOT level on rat blood. There were prepared 24 rats, divided into 6 groups, i.e. normal control group, positive control group, negative control group, and 3 test goups that were induced by INH and each was given the unripe pineapple fruits juice at 1 hour before and 1 hour after INH induction, with the dose of 2 mL and 4 mL. Treatment was conducted in 14 days, consecutively. To each of the rat groups, blood was taken out through the eye orbital sinus, i.e. on the first day, on the hour-0 and hour-3, and then the SGPT and SGOT level were measured. Later on, blood were taken out on the day-4, day-7, and day-14. On the rat blood that were taken out, were analyzed descriptively and statistically to determined the optimum dose and the time of the unripe pineapple fruits juice feeding in related with a hepatoprotective potency. Result showed that feeding of the unripe pineapple fruit juice 1 hour after INH induction with the dose of 2 mL during 14 days has the best hepatoprotective activity.
References
2. Kementerian Kesehatan Republik Indonesia. Keputusan Menteri Kesehatan Republik Indonesia No.328/Menkes/IX/2013 tentang Formularium Nasional. Jakarta: Kementrian Kesehatan Republik Indonesia; 2013.
3. Katzung BG. Farmakologi dasar dan klinik. Edisi 13. Jakarta: EGC;2016.
4. Teixeira RLF,Morato RG, Cabello PH, Muniz LM, Moreira AS, Kritski AL, et al. Genetic polymorphisms of NAT2, CYP2E1 and GST enzymes and the occurrence of antituberculosis drug-induced hepatitis in Brazilian tuberculosis patients. Mem Inst Oswaldo Cruz 2011;106(6): 74-276.
5. Chatuphonprasert W, K Jarukamjorn. Impact of six fruits-banana, guajava, mangosteen, pineapple, ripe mango, and ripe papaya-on murine hepatic cytochrome P450 activities. J o Appl Toxicol 2012;32:994-1001.
6. Jacques DT, Mark KT, Marius A. Biochemical effectiveness in liver detoxication of fresh pineapple (Ananas comosus) with wistar rats
7. Winarno FG. Kimia pangan dan gizi. Bogor: M-Brio Press; 1982.
8. Walraven JM. Computational and functional analysis of human and rat N-acetyltransferase genetic variants (dissertation). Louisville: Departement of Pharmacology and Toxicology University of Louisville School of Medicine; 2007.
9. Departemen Kesehatan Republik Indonesia. Pharmaceutical care untuk penyakit tuberculosis. Jakarta: Direktorat Bina Farmasi Komunitas dan Klinik. Direktorat Jendral Bina Farmasi dan Alat Kesehatan. Departemen Kesehatan Republik Indonesia; 2005.
10. Lisdiana, Widyaningsih S. Budidaya nanas, pengolahan, dan pemasaran. Solo: CV Aneka; 1997.
11. Syahrumsyah H . Pengaruh penambahan CMS dan tingkat kematangan buah nanas. Jurnal Teknologi Pertanian Universitas Mulawarman. 2010;6(1):1858-2419.
12. Malik D, C Bhattacharjee, TS Gouda. Pharmacological intervention of the fruit of plant Ananas comusus acting as hepatoprotective activity in
animal models. Indian J o Researc n Pharm and Biotech 2014;2(3):1167-1172.
13. Syamsudin, Darmono. Farmakologi eksperimen. Jakarta: UI Press; 2011.
14. Woodley M, Alison W. Pedoman pengobatan. Yogyakarta: Yayasan Essentia Medica dan Andi Offset; 1995.p. 473-475.
15. Dalimartha S. Ramuan tradisional untuk pengobatan hepatitis. Jakarta: Penebar Swadaya; 2006.p. 11-73, 114.
16. Suyono S, editor. Buku ajar ilmu penyakit dalam. Jilid III. Edisi III. Jakarta: Fakultas Kedokteran Universitas Indonesia; 2001.p.224-27.
17. Huang KC. The pharmacology of chinese herbs. Second edition. CRPress. Washington DC; 1999.p. 118, 127, 243, 256, 315, 480.
18. Poggi M, Barroso R, Pavan F, et al. New isoniazid complexes, promising agents against mycobacterium tuberculosis. J Mex Chem Soc. 2013;57(3):4.
19. Ngatidjan PS. Farmakologi dasar. Yogyakarta:FKUGM;2006.
20. Himawan R. Pengaruh pemberian ekstrak daun the hijau (Camellia sinensis) terhadap kadar SGPT tikus putih (Rattus norvegicus) yang diinduksi isoniazid (skripsi). Surakarta: Fakultas Kedokteran Universitas Sebelas Maret; 2008.
Licencing
All articles in Jurnal Ilmu Kefarmasian Indonesia are an open-access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which permits unrestricted non-commercial used, distribution and reproduction in any medium.
This licence applies to Author(s) and Public Reader means that the users mays :
- SHARE:
copy and redistribute the article in any medium or format - ADAPT:
remix, transform, and build upon the article (eg.: to produce a new research work and, possibly, a new publication) - ALIKE:
If you remix, transform, or build upon the article, you must distribute your contributions under the same license as the original. - NO ADDITIONAL RESTRICTIONS:
You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
It does however mean that when you use it you must:
- ATTRIBUTION: You must give appropriate credit to both the Author(s) and the journal, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
You may not:
- NONCOMMERCIAL: You may not use the article for commercial purposes.
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.