Efficacy Methanol Extract of Torbangun Leaves (Plectranthus amboinicus) in Epithelial Cell of Mammary Gland MCF-12A
Abstract
Torbangun (Plectranthus amboinicus) is an herbaceous plant that grows in the tropical region. Torbangun can increase breast milk and has antioxidant activity. The objective of this research was to evaluate the antioxidant and cytotoxic activities of methanol extract of torbangun leaves and to identify diff erentially expressed lactation genes upon incubation of MCF-12A human cell epithelial mammary gland with methanolic extract of torbangun leaves. The results showed that the methanolic extract of torbangun leaves had a scavenging activity of DPPH radical with EC50 value of 14.14 μg/ mL. At lower concentration of methanol extracts of torbangun leaves showed no potent cytotoxic eff ect on MCF-12A with IC50 value of 155.24 μg/mL. The methanolic extract of torbangun leaves increased genes expression of prolactin receptor and glucocorticoid receptor in human mammary gland epithelial cells MCF-12A.
References
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25. Gupta M, Shaw B. A double-blind randomized clinical trial for evaluation of galactogogue activity of Asparagus racemosus Willd. Iranian J Pharm Res. 2011.10(1):167-72.
26. Andarwulan N, Yuliana ND, Hasna E, Aziz SA, Davis TD. Comparative analysis of three torbangun clones (Plectranthus amboinicus (Lour.) Spreng) based on phenotypic characteristics and phenolic content. Am J Plant Sci. 2014.5:3673-83. http://dx.doi.org/10.4236/ ajps.2014.524383.
2. Damanik R, Wahlqvist ML, and Wattanapenpaiboon N. Lactagogue eff ects of Torbangun, a Bataknese traditional cuisine. Asia Pac J Clin Nutr. 2006.15(2): 267-74.
3. Bhatt P and Negi PS. Antioxidant and antibacterial activities in the leaf extracts of indian borage (Plectranthus amboinicus). Food Nutr Sci. 2012.3:146- 52. doi:10.4236/fns.2012.32022.
4. Mistry HD dan Williams P. The importance of antioxidant micronutrie nts in pregnancy.Oxidative Medicine and Cellular Longevity. 2011. doi:10.1155/2011/841749
5. Zarban A, Taheri F, Chahkandi T, Sharifzadeh G, Khorashadizadeh M. Antioxidant and radical scavengin activity of human colustrum tranditional and mature milk. J Clin Biochem Nutr. 2009.45:150–4. 6. Harborne JB. Metode fi tokimia penuntun cara modern menganalisa tumbuhan. ITB Bandung. 1984.
7. Majd MH, Rajaei A, Bashi DS, Mortazavi SA, Bolourian S. Optimization of ultrasonic-assisted extraction of phenolic compounds from bovine pennyroyal (Phlomidoschema parviflorum) leaves using response surface methodology. Ind Crops Prod. 2014.57:195–202.
8. Shah MD and Hossain MA. Total fl avonoids content and biochemical screening of the leaves of tropical endemic medicinal plant Merremia borneensis. Arab J Chem. 2014.7:1034–8.
9. Aranda RS, P´erez-L ´opez LA, L´opez-Arroyo J Alan´ıs-Garza BA, and Torres NW. Antimicrobial and antioxidant activities of plants from northeast of Mexico. 2011. doi:10.1093/ecam/nep127.
10. Ayoub IM, El-Shazly M, Lu M-C, Singab ANB. Antimicrobialand cytotoxic activities of the crude extracts of Dietes bicolor leaves, fl owers and rhizomes. South African J Botany. 2014.97:101.
11. Pillai PG, Suresha P, Mishrab G, Annapurnaa M. Evaluation of the acute and sub acute toxicity of the methanolic leaf extract of Plectranthus amboinicus (Lour) Spreng in balb C mice. Eur J Exp Biol. 2011.1(3):236-45.
12. Wadekar RR, Wani NS, Bagul UB, Bagul SD, Bedmutha RK. Phytochemical investigation and screening of invitro anthelmentic activity of Plectranthus amboinicus leaves extracts. Int J Pharmacognosy Phytochem Res. 2010.3(2):35-8.
13. Chavan UD, Amarowicz R. Eff ect of various solvent systems on extraction of phenolics, tannins and sugars from beach pea (Lathyrus maritimus L.). Int Food Res J. 2013. 20(3):1139-44.
14. Uma M, Jothinayaki S, Kumaravel S, Kalaiselv P. Determination of bioactive components of Plectranthus amboinicus Lour by GC–MS analysis. N Y Sci J. 2011.4(8):66-9.
15. Khalid AK, El-Gohary AE. Effect of seasonal variations on essential oil production and composition of Plectranthus amboinicus (Lour.) grow in Egypt. Int Food Res J. 2014.21(5):1859-62.
16. Surya A, Jose C, Teruna HY. Studi aktivitas antioksidan dari ekstrak metanol dan etil asetat pada daun bangunbangun (Plectranthus amboinicus). J Ind Chem Acta. 4. 2013.17. Wojdyło A, Oszmian´ski J, dan Czemerys R. Antioxidant activity and phenolic compounds in 32 selected herbs. Food Chem. 2007.105:940–9.
18. El-Hawary SS, El-sofany RH, Abdel-Monem AR, Ashour RS, Sleem AA. Polyphenolics content and biological activity of Plectranthus amboinicus (Lour.) Spreng growing in Egypt (Lamiaceae). Phcog J. 2012.4(32):45-54.
19. Graidist P, Martla M, Sukpondma Y. Cytotoxic activity of Piper cubeba extract in breast cancer cell lines. Nutrient. 2015.7: 2707-18. doi:10.3390/nu7042707.
20. Morales FC, Hayashi Y, Pelt CS, Georgescu M-M. NHERF1/EBP50 controls lactation by establishing basal membrane polarity complexes with prolactin receptor. Cell Death Dis. 2012.3:391. doi:10.1038/ cddis.2012.131.
21. Yonekura S, Miyazaki S, Tokutake Y. Comparative expression profi ling of lactogenic hormone receptor and it’s signaling molecules of bovine mammary glands during lactation. J Ani Sci. 2015.5:106-113.
22. Wintermantel TM, Bock D, Fleig V, Greiner EF, Schutz G. The epithelial glucocorticoid receptor is required for the normal timing of cell proliferation during mammary lobuloalveolar development but is dispensable for milk production. Molecular Endocrinology. 2005.19(2):340–9.
23. Chiba T, Kimura S, Takahashi K, Morimoto Y, Sanbe A, Ueda H, Kudo K. Serotonin suppresses β-casein expression via inhibition of the signal transducer and activator of transcription 5 (Stat5) protein phosphorylation in human mammary epithelial cells MCF-12A. Biol Pharm Bull. 2014.37(8):1336–40.
24. Okasha MAM, Abubakar MS, Bako IG. Study of the eff ect of aqueous Hibiscus sabdariff a Linn seed extract on serum prolactin level of lactating female Albino rats. Eur J Sci Res. 2008.22(4):575-83.
25. Gupta M, Shaw B. A double-blind randomized clinical trial for evaluation of galactogogue activity of Asparagus racemosus Willd. Iranian J Pharm Res. 2011.10(1):167-72.
26. Andarwulan N, Yuliana ND, Hasna E, Aziz SA, Davis TD. Comparative analysis of three torbangun clones (Plectranthus amboinicus (Lour.) Spreng) based on phenotypic characteristics and phenolic content. Am J Plant Sci. 2014.5:3673-83. http://dx.doi.org/10.4236/ ajps.2014.524383.
Published
2017-04-30
How to Cite
TAFZI, FITRY et al.
Efficacy Methanol Extract of Torbangun Leaves (Plectranthus amboinicus) in Epithelial Cell of Mammary Gland MCF-12A.
JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 15, n. 1, p. 17-24, apr. 2017.
ISSN 2614-6495.
Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/447>. Date accessed: 26 apr. 2024.
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