Dampak Positif Fraksi Etil Asetat dari Kalanchoe pinnata terhadap Antibodi Anti-Smith dan Sel T Reg pada Mencit Lupus

  • NIKEN INDRIYANTI MULAWARMAN UNIVERSITY
  • JUNAIDI KHOTIB UNIVERSITAS AIRLANGGA
  • JOEWONO SOEROSO UNIVERSITAS AIRLANGGA

Abstrak

Kalanchoe pinnata (Lmk) Pers memiliki banyak aktivitas, seperti antioksidan, antiinfl amasi, dan imunosupresan. Berdasarkan penelitian sebelumnya, ekstrak air daun Kalanchoe pinnata (Lmk) Pers memiliki efek memperbaiki struktur dan fungsi ginjal pada model lupus nefritis. Senyawa aktif yang diduga adalah senyawa-senyawa golongan fl avonoid yang masuk ke dalam fraksi etil asetat sesuai metode fraksinasi Cruz(1). Tujuan penelitian ini adalah membuktikan aktivitas fraksi etil asetat Kalanchoe pinnata (Lmk) Pers (EF-KP) untuk menurunkan kadar antibodi Anti-Smith dan mengatur fungsi regulasi sel T regulator CD4+CD25+. Kelompok uji terdiri dari kontrol negatif (placebo), FE-KP, dan kontrol positif (siklofosfamid). Kadar anti-Sm diukur menggunakan indirect ELISA. Selanjutnya sel-sel limpa diisolasi untuk pengukuran sel T reg CD4+CD25+ dengan metode fl ow cytometry. Hasil penelitian menunjukkkan bahwa ada penurunan rata-rata kadar anti-Sm yang cukup untuk menstabilkan kondisi model lupus. Hasil pengukuran T reg menunjukkan peningkatan jumlah marker sel T reg CD4+CD25+. Peningkatan fungsi regulasi ini mampu menghambat reaktivitas autoantigen, sehingga mencegah kerusakan jaringan dan organ pada penyakit lupus. Namun, penelitian selanjutnya perlu dilakukan untuk mengamati mekanisme kerja FE-KP pada biomarker lupus yang lain.

Referensi

1. Cruz EA, Reutera S, Martina H, Dehzada N, Muzitano M, Costa SS, et al. Kalanchoe pinnata inhibits mast cell activation and prevents allergic airway disease. Phytomedicine. 2012.19:115– 21.

2. Gupta R, Lohani M, Arora S. Anti-inflammatory activity of the leaf extracts/fractions of Bryophyllum pinnatum. Int J Pharm Sci Rev & Res. 2010.3(1):16-8.

3. Ferreira RT, Coutinho MAS, Carmo Malvar D, Costa EA, Florentino IF, Costa SS, et al. Mechanisms underlying the antinociceptive, antiedematogenic, and anti-infl ammatory activity of the main fl avonoid from Kalanchoe pinnata. Evid Base Compl Alternative Med. 2014. Article ID 429256, 8 pages.

4. Cruz EA, Da-Silva SAG, Muzitano MF, Silva PMR, Costa SS, Rossi-Bergmann B. Immunomodulatory pretreatment with Kalanchoe pinnata extract and its quercitrin fl avonoid eff ectively protects mice against anaphylactic shock. Int Immunopharmacol. 8:1616-21.

5. Bergmann R, Costa SS, Borges MBS, Silva SA, Noleto GR, Souza MLM. Immunosuppressive eff ect of the aqueous extract of Kalanchoe pinnata in mice. Phytotherapy Res. 1994.8:399-402.

6. Coutinho MA, Muzitano MF, Cruz EA, Bergonzi MC, Kaiser CR, Tinoco LW, Bilia AR, Vincieric FF, Rossi-Bergmann B, Costa SS. Flowers from Kalanchoe pinnata are a rich source of T cell-suppressive fl avonoids. Nat Product Commun. 2012.7(2):175-8.

7. Yu C, Gershwin ME, Chang C. Diagnostic criteria for systemic lupus erythematosus: A critical review. J of Autoimmun. 2014.5:48-59. 8. Reeves HW, Lee PY, Weinstein JS, Satoh M, Lu L. Induction of autoimmunity by pristane and other naturally occurring hydrocarbons. Trends in Immunol. 2009.30(9):455–64.

9. Rottman JB, Willis CR. Mouse models of systemic lupus erythematosus reveal a complex pathogenesis. Vet Pathol. 2010.47(4):664-76.

10. Scheinecker C, Bonelli M, Smolen JS. Pathogenetic aspects of systemic lupus erythematosus with an emphasis on regulatory T cells. J Autoimmun. 2010.35:269-75.

11. Nascimento LB, Leal-Costa MV, Coutinho MA, Moreira Ndos S, Lage CL, Barbi Ndos S, et al. Increased antioxidant activity and changes in phenolic profile of Kalanchoe pinnata (Lamarck) Persoon (Crassulaceae) specimens grown under supplemental blue light. Photochem and Photobiol. 2013.89(2): 391-9.

12. Satoh M, Kuroda Y, Yoshida H, Behney KM, Mizutani A, Akaogi J, et al. Induction of lupus autoantibodies by adjuvants. J Autoimmun. 2003.2:1–9.

13. Pawar RD, Goilav B, Xia Y, Zhuang H, Herlitz L, Reeves WH, Putterman C. Serum autoantibodies in pristane-induced lupus are regulated by neutrophil gelatinase-associated lipocalin. Clin Immunol. 2014.154:49–65.

14. Calvani N, Satoh M, Croker BP, Reeves WH, Richards HB. Nephritogenic autoantibodies but absence of nephritis in Il-12p35–defi cient mice with pristaneinduced lupus. Kidney Int. 2003.64:897–905.

15. Rifa’i M. Protective eff ect of CD4+CD25+ regulatory T cells on mice model of rheumatoid arthritis. Annales Bogorienses. 2011.15(1):48-57.

16. Qarni UA, Rifa’i M. Uji aktifitas biologis fraksi etanol daun sambiloto (Andrographis paniculata) terhadap perubahan kuantitatif sel T regulator pada mencit BALB/c (Mus musculus). Jurnal Biotropika. 2013.1(5):15-21.

17. Muzitano MF, Bergonzi MC, Melo GOD, Lage CLS, Bilia AR, et al. Infl uence of conditions, season of collection and extraction method on the content of antileishmanial fl avonoids from Kalanchoe pinnata. J Ethnopharmacol. 2011.133:132-7.

18. Wallace DC and Hahn BH. Dub ois’s lupu serythematosus and related syndromes. New York: Elsevier Saunder; 2013:237-51.

19. Satoh M, Reeves WH, Induction of lupus-associated injection of pristane. J Exp Med. 1994.180(6):23-41.

20. Leiss H, Niederreiter B, Bandur T, Schwarzecker B, Steiner G, et al. Pristane-induced lupus as a model of human lupus arthritis: evolvement of autoantibodies, internal organ and joint inflammation. Lupus. 2013.22:778–92.

21. Amelsfort JM, Jacobs KM, Bijlsma JW, Lafeber FP, Taams LS. CD4+CD25+ regulatory T cells in rheumatoid arthritis. Differences in the presence, phenotype, and function between peripheral blood and synovial fl uid. Arthritis Rheumatol. 2004.50:2775-85.

22. Bleesing MR, Brown SE, Straus JK, Siegel MD, Johnson M. Immunophenotypic profi les in families with autoimmune lymphoproliferative syndrome. Blood. 2001.98(8):2466-73.

23. Chatenoud LB, Salomon, Bluestone JA. Suppressor T cells-they’re back and critical for regulation of autoimmunity. Immunol Rev. 2001.182:149-63.

24. Chen W, Jin N, Hardegen KJ, Lei I, Marinos. Conversion of peripheral CD4+CD25+ naive T cells to CD4+CD25+ regulatory T cells by TGF-beta induction of transcription factor Foxp3. J Exp Med. 2003.198:1875-86.

25. Malek TR, Bayer AR. Tolerance, not immunity, crucially depends on IL-2. Nat Rev Immunol. 2004. 4:665-74.

26. Seddon B, Mason D. The third function of the thymus. Immunol. 2001.21:95-9.

27. Wan Y, Flavell RA. Identifying Foxp3-expressing suppressor T cells with a bicistronic reporter. Proc Natl. Acad Sci. 2005.102:5126–31.
Diterbitkan
2017-04-30
##submission.howToCite##
INDRIYANTI, NIKEN; KHOTIB, JUNAIDI; SOEROSO, JOEWONO. Dampak Positif Fraksi Etil Asetat dari Kalanchoe pinnata terhadap Antibodi Anti-Smith dan Sel T Reg pada Mencit Lupus. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 15, n. 1, p. 57-62, apr. 2017. ISSN 2614-6495. Tersedia pada: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/454>. Tanggal Akses: 18 sep. 2025
##submission.howToCite.citationFormats##
Terbitan
Vol 15 No 1 (2017): JIFI
Bagian
Articles
##submission.license##

Licencing
All articles in Jurnal Ilmu Kefarmasian Indonesia are an open-access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which permits unrestricted non-commercial used, distribution and reproduction in any medium.

This licence applies to Author(s) and Public Reader means that the users mays :

  • SHARE:
    copy and redistribute the article in any medium or format
  • ADAPT:
    remix, transform, and build upon the article (eg.: to produce a new research work and, possibly, a new publication)
  • ALIKE:
    If you remix, transform, or build upon the article, you must distribute your contributions under the same license as the original.
  • NO ADDITIONAL RESTRICTIONS:
    You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

It does however mean that when you use it you must:

  • ATTRIBUTION: You must give appropriate credit to both the Author(s) and the journal, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.

You may not:

  • NONCOMMERCIAL: You may not use the article for commercial purposes.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.