Anti–Platelet Aggregation Effect from Ethanol Extract of Salam Leaves (Syzygium polyanthum (Wight) Walp.) on Mice

  • RIKA SARI DEWI UNIVERSITAS PANCASILA
  • NI MADE DWI SANDHIUTAMI UNIVERSITAS PANCASILA
  • SULISTYO RAHARJO UNIVERSITAS PANCASILA

Abstract

Salam leaves (Syzygium polyanthum (Wight) Walp.) contains flavonoids, volatile oil and phenolic acid that can inhibiting platelet aggregation induced by ADP (adenosine diphospate), arachidonic acid, epinephrine, thrombin, collagen and aff ecting the activity enzyme ectonucleotidase so it can be used as anti-platelet drug. The purpose of this study was to investigated 70% ethanol extract of salam leaves have anti-platelet aggregation eff ect and increase of dose can also increase anti-platelet aggregation. This study was performed in 5 groups, each group consisted of 5 mice there were: groups of positive control, normal control, 70% ethanol extract leaves doses (150, 300, 450) mg/ kg, each group is given orally once daily during 8 days and measurements of parameters at day-0 and day-9th. The results of this study was 70% ethanol extract of leaves of salam leaves doses (150,300, 450) mg/kgBW showed the effects prolong bleeding time, coagulation time, enhance the decreased plasma absorbance after the addition of ADP. From the measurement parameter bleeding time, coagulation time and measurement decreased plasma absorbance after the addition of ADP that 70% ethanol extract of salam leaves doses 150, 300 and 450 mg/kgBW have anti-platelet aggregation eff ect and increase of dose was able to increase the eff ect anti-platelet aggregation.

References

1. Underwood JCE. Patologi umum dan sistematik.Editor Bahasa Indonesia Sarjadi . Ed 2. Jakarta: Penerbit Buku Kedokteran EGC; 1999.

2. Off ermans, Stefan. Activation of platelet function through G protein–coupled receptors. Circulation Research 2006.99:1293–1304.

3. Saeed SA, Gilani AH. Antithrombotic activity of clove oil. Journal Pakistan Medical Association. 1994.44:112.

4. Syamsul hidayat, Sugati Sri, Hutapea, Ria Johnny. Inventaris tanaman obat Indonesia I. Jakarta: Departemen Kesehatan RI, Badan Penelitian dan Pengembangan Kesehatan; 1991. 238.

5. Setyawati R. Uji aktivitas antiplatelet & trombolitik ekstrak etanol kulit buah jeruk purut (Citrus hystrix D.C) in vitro [skripsi]. Jember: Fakultas Farmasi Universitas Jember; 2015.

6. Carson CF and Riley TV. Antimicrobial activity of the major components of the essential oil of Melaleuca alternifolia. Journal of Applied Bacteriology. 1995.78: 264–9.

7. Saeed SA, Simjee RU, Shamim G, Gilani AH. Eugenol: a dual inhibitor of platelet- activating factor and arachidonic acid metabolism. Phytomedicine. 1995. 2(1):23-8.

8. Chen SJ, Wang MH, Chen IJ. Antiplatelet and calcium inhibitory properties of eugenol and sodium eugenol acetate. Gen Pharmac. 1996.27(4):629-33.

9. Oh WJ, Endale M, Park SC, Cho YJ, Rhee MH. Dual roles of quercetin in platelet phosphoinositide–3 kinase and MAP kinases inhibition and cAMP-dependent vasodilator – stimulated phosphoprotein stimulation. Evidence Based Complementary and Alternative Medicine. 2012:1-10.

10. Wright Bernice, Moraes LA, Kemp CF, Mullen William, Crozier Alan, Lovegrove JA, et al. A structural basis for the inhibition of collagen-stimulated platelet function by quercetin and structurally related flavonoids. British Pharmacology Journal. 2010.159:1312-25. doi:10.1111/j.1476-5381.2009.00632.x.

11. YS Elin, IS Joseph, F Nurul. Efek antiagregasi platelet ekstrak etanol buah mengkudu (Morinda citrifolia L.), rimpang jahe merah (Zingiber offi cinale var. Sunti Val) dan kombinasinya pada mencit jantan galur Swiss Webster. JKM. 2008.7:1-18.

12. KC Srivastava, T Mustafa. Spices: Antiplatelet activity and prostanoid metabolism. In: Prostaglandins leukotrimes and essential fatty acids: Reviews. 1989. 38:255-66.

13. Ghasemzadeh A, Jaafar HZE, Rahmat A. Variation of the phytochemical constituents and antioxidant activities of Zingiber offi cinale var. rubrum Theilade associated with diff erent drying methods and polyphenol oxidase activity. Molecules. 2016;21:780.

14. Samudra A. Karakterisasi ekstrak etanol daun salam (Syzygium polyanthum Wight) dari tiga tempat tumbuh Indonesia [Skripsi]. Jakarta : Fakultas Kedokteran dan Ilmu Kesehatan Program Studi Farmasi UIN Syarif Hidayatullah Jakarta; 2014.

15. Sukandar EY, Sigit JI, Fitriani, Nurul. Efek antiagregasi platelet ekstrak air bulbus bawang putih (Allium sativum L.), ekstrak etanol rimpang kunyit (Curcuma domestica Val.) dan kombinasinya pada mencit jantan galur Swiss Webster. Majalah Farmasi Indonesia. 2008.19(1):1–11.

16. Gresele P, Fuster V, Lopez JA, Page CP, Vermylen J, editor. Platelets in hematologic & cardiovascular disorder. New York : Cambridge press; 2008. 87:293–5.
Published
2017-04-30
How to Cite
DEWI, RIKA SARI; SANDHIUTAMI, NI MADE DWI; RAHARJO, SULISTYO. Anti–Platelet Aggregation Effect from Ethanol Extract of Salam Leaves (Syzygium polyanthum (Wight) Walp.) on Mice. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 15, n. 1, p. 31-37, apr. 2017. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/457>. Date accessed: 24 nov. 2024.
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Vol 15 No 1 (2017): JIFI
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