Isolation and Identification of Chemical Compounds as Anticancer from Leaves of Kopasanda (Chromolaena odorata (L.))

  • MUHAMMAD FITRAH UNIVERSITAS ISLAM NEGERI MAKASAR
  • HENDIG WINARNO UNIVERSITAS PANCASILA
  • PARTOMUAN SIMANJUNTAK UNIVERSITAS PANCASILA

Abstract

Isolation and identifi cation of chemical compound as anticancer from the leaves of Kopasanda (Chromolaena odorata L.) had been done. Extraction was conducted successfully with n-hexane, ethylacetate and ethanol by maceration, and by refl ux for water solvent. Fractionation of ethylacetate extract by column chromatography (SiO2). n-hexane-ethylacetate = 10:1 ~ 1:1; ethylacetate). n-hexaneethylacetate = 5:1) gave one pure isolated fraction fr. 5-3 with inhibition activity against leukemia cell line L1210 at concentration 1.515 ppm. Chemical structure determination was done based on spectral data interpretation of Infra Red, 1D NMR (1H, 13C) and 2D NMR (H-H COSY, HMQC and HMBC) and mass spectra (LC-MS). Pure isolated fraction fr. 5-3 was deducted as methyl ether naragenine.

References

1. Kimball, J W. Biologi. Terjemahan oleh Hj. Siti Soetarmi Tjitrosoepomo dan Nawangsari Sugiri. Jakarta: Erlangga; 1983.

2. Anderson, R N. Deaths: Leading causes for national vital statistics reports. 1973.

3. Benjamin, VT. Phytochemical and antibacterial studies on the essential oil of Euphatorium odoratum. Pharmaceutical Biology. 2011.

4. Ngozi Igboh M, Jude Ikewuchi C and Catherine, Ikewuchi C. Chemical profi le of Chromolaena odorata L. (King and Robinson) leaves. Pakistan Journal of Nutrition 8. 2009.

5. Molyneux, P. The use of stable free radikal diphenylpicrilhidrazyl (DPPH) for estimating antioxidant activity. Journal Science of Technology. 2004. 26(2): 211-9.

6. Zachariades C.Biological control of tropical weeds using Arthropods: Chromolaena odorata (L.). Cambridge University Press; 2009.130-63.

7. Mans DR Rocha A B, Schwartsmann G. Anticancer drug discovery and development in Brazil: Targeted plant collection as a rational strategy to acqvire candidate anticamcer compounds. Oncologist. 2000.185-98.

8. Sumarny R. Karakteristik aktivitas antiproliferasi sel lestari tumor dan aktivitas fugositosis secara in vivo dari fraksi bioaktif rimpang temu putih (Curcuma zedoria (Christm) Roscoe). Institut Pertanian Bogor., 2006.

9. Akinmoladun, Afolabi C, Ibukun E O, dan Ologe, I A. Phytochemical constituents and antioxidant properties of extracts from the leaves of Chromolaena odorata. Scientifi c Research and Essay. Vol 2. 2007.

10. Prawiradiputra B R, Ki Rinyuh (Chromolaena odorata (L.) R. M. King & H. Robinson): Gulma padang rumput yang merugikan. Bogor: Balai Penelitian Ternak. 2007.

11. Kiaris HU, Understanding carcinogenesis an introduction to the moleculer basic of cancer. Willeyvch Verloeg.

12. Weinhem king SB. Cancer Biologi second edition Pearson Education. Lowlou. 2006.

13. Silverstein R M, Webster F X, Kiemle D J. Spectrometric identifi cation of organic compounds. New York: John Willey and Sons Inc. 7th edition; 2005. 78-9, 215-7.

14. Widyastuti N. Pengukuran aktivitas antioksidan dengan metode DPPH, CUPRAC dan FRAP serta kolerasinya dengan fenol dan fl avonoid pada enam tanaman. Bogor: Institut Pertanian Bogor-Press; 2010.

15. Sipayung A R D, De Chenon and P S Sudharto. Observations on Chromolaena odorata (L.) R.M. King and H. Robinson in Indonesia. Second International Workshop on the Biological Control and Management of Chromolaena odorata. Biotrop. Bogor. 1991.

16. Winarno H dan Katrin E. Aktivitas sitotoksik fraksi ekstrak etil asetat kulit batang mahkota dewa (Phaleria macrocarpa (Scheff) Boerl) terhadap sel kanker manusia. Majalah Obat Tradisional. 2008:120-7.

17. Simanjuntak P. Aplikasi spektrometri resonansi magnetic inti 2 dimensi untuk analisis tumbuhan obat tradisional Indonesia. Jurnal Imu Kefarmasian Indonesia. 2003:1-7.

18. Jang D S, Cuendet MPawlus A D, Kardono L B S, Kawanishi K, Farnsworth R, Fong H H S, Pezzuto J M and Kinghorn A D. Potential cancer chemopreventive constituents of the leaves of Macaranga triloba. Phytochemistry. 2004.65:345-50.

19. Dewick and Paul M. Medical natural product a biosynthetic approach. 2nd Ed. New York: John Wiley and Sons; 2002.

20. Beutler J A, Shoemaker R H, Johnson T and Boyd M R. Cytotoxic geranyl stilbenes from Macaranga schweinfurthii. J Nat Prod. 2008.61:1509-12.
Published
2017-04-30
How to Cite
FITRAH, MUHAMMAD; WINARNO, HENDIG; SIMANJUNTAK, PARTOMUAN. Isolation and Identification of Chemical Compounds as Anticancer from Leaves of Kopasanda (Chromolaena odorata (L.)). JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 15, n. 1, p. 77-81, apr. 2017. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/459>. Date accessed: 22 dec. 2024.
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Vol 15 No 1 (2017): JIFI
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