α-GLUCOSIDASE INHIBITORY ACTIVITY AND FORMULATION OF SOLID LIPID NANOPARTICLE CONTAINING ETHANOL 70% STANDARDIZED EXTRACT OF KUMIS KUCING LEAVES (Orthosiphon stamineus Benth.)

  • Risma Marisi Tambunan UNIVERSITAS PANCASILA
  • Winda Dwi Juliyanti UNIVERSITAS PANCASILA

Abstract

Kumis kucing leaves (Orthosiphon stamineus Benth.) are one type of plants that is able to inhibit the activity of α-glucosidase enzyme. This study aims to formulate Solid Lipid Nanoparticle from ethanol 70% standardized extract of kumis kucing leaves and which give α-glucosidase enzyme inhibitory activity. The result of SLN characteristic for particle size of formula I, II and III shows the result of 51,60 nm; 12.81 nm; 11.87 nm and zeta potential of formula I, II and III show the results -27.67; -10,7; -15.0, respectively. The results of the α-glucosidase enzyme inhibitory activity of the standardized extract, SLN formula I, II and III respectively showed IC50 of 132.9 ppm; 130.3 ppm; 131.4 ppm; 132.2 ppm and Acarbose as comparison showed IC50 of 50.0 ppm. Data processing using t-test statistically at α = 0,05 showed that extract and SLN of formula I had significant difference, thus the best SLN formula was formula I with concentration of tween 80 and propylenglycol of 6% and 10%.

References

1. Restyana Noor F. Diabetes melitus tipe 2. J Majority.2015;5(4):93-94.

2. Victoria Cyntia YS. Pengaruh pemberian ekstrak daun kumis kucing (Orthosiphon aristatus) terhadap penurunan kadar glukosa darah tiku wistar yang diinduksi aloksan. Laporan hasil karya tulis ilmiah.2012:3.

3. Ameria. Penapisan fitokimia, uji toksisitas dengan metode BSLT dan uji aktivitas penghambatan enzim α-glukosidase secara in-vitro ekstrak etanol 70% dari delapan jenis simplisia (skripsi). Jakarta: Fakultas Farmasi Universitas Pancasila;2014. h.70-90.

4. Aryanto, Yateman. Nano di indonesia. Jakarta: Keduputian Perkembangan Riptek Kementrian Negara Riset dan Teknologi;2007. h.21-26.

5. Gupta, Ram B, Kompella, Uday B. Nanoparticle technology for drug delivery. volume 159. New York: Taylor & Francis Group;2006. h.6-45.

6. Anu M, Sandeep K. Solid lipid nanoparticles – as novel lipid based nanocarriers for drugs. Internasional Journal of Advanced Research:2014;2:433-441.

7. Tegar GR, Noorma R, Tristiana E. Characterisation of solid lipid nanoparticles p-methoxycinnamic acid (SLN-PMCA) formulated with different lipid component: stearic acid and cetyl alcohol. Pharma Scientia.2012;1(1):23-25.
Published
2018-04-27
How to Cite
TAMBUNAN, Risma Marisi; JULIYANTI, Winda Dwi. α-GLUCOSIDASE INHIBITORY ACTIVITY AND FORMULATION OF SOLID LIPID NANOPARTICLE CONTAINING ETHANOL 70% STANDARDIZED EXTRACT OF KUMIS KUCING LEAVES (Orthosiphon stamineus Benth.). JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 16, n. 1, p. 45-48, apr. 2018. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/485>. Date accessed: 26 sep. 2022. doi: https://doi.org/10.35814/jifi.v16i1.485.
Section
Articles