Pembuatan, Karakterisasi dan Uji In Vitro Nanopartikel Emas Berbasis Konjugat Gom Arab-Vinkristin

  • Ratih Dyah Pertiwi UNIVERSITAS INDONESIA
  • Joshita Djajadisastra UNIVERSITAS INDONESIA
  • ABDUL MUTALIB UNIVERSITAS PADJADJARAN
  • Anung Pujiyanto UNIVERSITAS ESA UNGGUL

Abstract

Gold nanoparticles (AuNP) are potentially developed as nanomedicine because AuNP is easily synthesized, functionalized, and biocompatible. With gum arabic as a stabilizer, vincristine was conjugated with gold nanoparticles. As a reducing agent, it used 0.02 M Natrium Boro Hidrat (NaBH4)  solution. Gold nanoparticles (AuNP) coated with conjugated gum Arabic (GA) and vincristine (VCR) were successfully synthesized and characterized. The conjugation of GA-VCR and AuNP displayed a narrow hydrodynamic particle size distribution with average size < 100 nm by TEM and  PSA (particle size analyzer). We investigated the cytotoxic activity of conjugated vincristine-gum arabic-gold nanoparticle by tetrazolium salt assay (MTT) using cancer cell line CCR-CEM. Cytotoxic activity of conjugated VCR-GA-AuNP before and after purification by Size Exclusion Chromatography (SEC), against leukemia cell line CCRF-CEM, was described by IC50 value. All formulation had a cytotoxic of activity with IC50 <20 μg/ml. The IC50 of samples against CCRF cell line were 1,026 μg/mL and  2,607 ug/mL, respectively.

References

1. Kojima C, Hirano Y, Yuba E, Harada A, Kono K. Preparation and characterization of complexes of liposomes with gold nanoparticles. Colloids Surf B Biointerfaces. 2008 Oct;66(2):246–52.

2. Zhang L, Yu F, Cole AJ, Chertok B, David AE, Wang J, et al. Gum Arabic-Coated Magnetic Nanoparticles for Potential Application in Simultaneous Magnetic Targeting and Tumor Imaging. AAPS J. 2009 Dec;11(4):693–9.

3. Pujiyanto A, Mujinah, Lubis H, Witarti, Setiawan H, Dede K, et al. Penggunaan Gum Arab sebagai Stabilisator Nano Partikel emas untuk Diagnosis dan Terapi Kanker. Pros Semin Nas Sains Dan Pendidik IX Fak Sains Dan Mat UKSW Salatiga. 2014 Jun 21;V no 1:486–90.

4. Doyle A, Griffiths J. Cell and Tissue Culture for Medical Research. British, Wiley; 2000. 409-410 p.

5. Vigderman L, Zubarev ER. Therapeutic platforms based on gold nanoparticles and their covalent conjugates with drug molecules. Adv Drug Deliv Rev. 2013 May;65(5):663–76.

6. Esumi, K., Suzuki, A., Yamahira, A., dan Torigoe, K. Role of Poly (amidoamine) dendrimers for preparing nanoparticles of gold, platinum, and silver. ‘langmuir 16. 2000; 2604-2608.

7. Wu C-C, Chen D-H. Facile green synthesis of gold nanoparticles with gum arabic as a stabilizing agent and reducing agent. Gold Bull. 2010;43(4):234–240.

8. Wang X, Wang Y, Chen ZG, Shin DM. Advances of Cancer Therapy by Nanotechnology. Cancer Res Treat. 2009;41(1):1.

9. Mohanraj VJ, Chen Y. Nanoparticles-a review. Trop J Pharm Res. 2006;5(1):561–573.

10. Suffness M, J. M. Pezzuto. Assays Related to Cancer Drug Discovery. In: Methods in Plant Biochemistry: Assays for Bioactivity. London: Academic Press; 1990. p. 71–133.
Published
2018-04-27
How to Cite
PERTIWI, Ratih Dyah et al. Pembuatan, Karakterisasi dan Uji In Vitro Nanopartikel Emas Berbasis Konjugat Gom Arab-Vinkristin. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 16, n. 1, p. 6-11, apr. 2018. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/486>. Date accessed: 15 dec. 2019. doi: https://doi.org/10.35814/jifi.v16i1.486.
Section
Articles