Pembuatan, Karakterisasi dan Uji In Vitro Nanopartikel Emas Berbasis Konjugat Gom Arab-Vinkristin

  • Joshita Djajadisastra UNIVERSITAS INDONESIA


Gold nanoparticles (AuNP) are potentially developed as nanomedicine because AuNP is easily synthesized, functionalized, and biocompatible. With gum arabic as a stabilizer, vincristine was conjugated with gold nanoparticles. As a reducing agent, it used 0.02 M Natrium Boro Hidrat (NaBH4)  solution. Gold nanoparticles (AuNP) coated with conjugated gum Arabic (GA) and vincristine (VCR) were successfully synthesized and characterized. The conjugation of GA-VCR and AuNP displayed a narrow hydrodynamic particle size distribution with average size < 100 nm by TEM and  PSA (particle size analyzer). We investigated the cytotoxic activity of conjugated vincristine-gum arabic-gold nanoparticle by tetrazolium salt assay (MTT) using cancer cell line CCR-CEM. Cytotoxic activity of conjugated VCR-GA-AuNP before and after purification by Size Exclusion Chromatography (SEC), against leukemia cell line CCRF-CEM, was described by IC50 value. All formulation had a cytotoxic of activity with IC50 <20 μg/ml. The IC50 of samples against CCRF cell line were 1,026 μg/mL and  2,607 ug/mL, respectively.


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How to Cite
PERTIWI, Ratih Dyah et al. Pembuatan, Karakterisasi dan Uji In Vitro Nanopartikel Emas Berbasis Konjugat Gom Arab-Vinkristin. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 16, n. 1, p. 6-11, apr. 2018. ISSN 2614-6495. Available at: <>. Date accessed: 15 dec. 2019. doi: