Partial Validation of High Performance Liquid Chromatography for Analysis of Isoniazid in Rat Plasma

  • Novi Yantih UNIVERSITAS PANCASILA
  • Siti Hafilah UNIVERSITAS PANCASILA
  • Yahdiana Harahap UNIVERSITAS INDONESIA
  • WAHONO SUMARYONO UNIVERSITAS PANCASILA
  • Rianto Setiabudy UNIVERSITAS INDONESIA

Abstract

Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis. Nicotinic acid derivatives such as isoniazid have the strongest anti-tuberculosis properties. For pharmacokinetics studying of isoniazid (INH), a method is needed to determine the levels of INH in plasma. Objective: The aim of this research is to partial validate of high performance liquid chromatography (HPLC) for analysis of INH in rat plasma. Method: For the preliminary study, rat plasma was used. The HPLC system used is a stationary phase C18 with length 250mm and temperature of 30°C, mobile phase hexane sulphonate acid 20mM pH 2.47–methanol (65:35). The analytical parameters in partial validated were linearity, lower limit of quantification (LLOQ), precision, accuracy, and recovery. Results: The results of linearity test of INH showed r value of 0.9996. LLOQ of this method was 0.1258μg/mL. The resulting accuracy and precision value met FDA requirements with a percent recovery ranging from 96.57–107.99%. Conclusion: The HPLC system was a valid method for analysis of INH in rat plasma.

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Published
2018-04-27
How to Cite
YANTIH, Novi et al. Partial Validation of High Performance Liquid Chromatography for Analysis of Isoniazid in Rat Plasma. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 16, n. 1, p. 67-71, apr. 2018. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/497>. Date accessed: 26 sep. 2022. doi: https://doi.org/10.35814/jifi.v16i1.497.
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Articles