Khasiat dan Profil Kromatogram Fraksi Aktif dari Ekstrak Kulit Buah Manggis (Garcinia mangostana L.) yang Diiradiasi

  • Ermin Katrin PATIR-BATAN JAKARTA SELATAN
  • Setiananda Jacobs UNIVERSITAS PANCASILA
  • Hendig Winarno PATIR-BATAN JAKARTA SELATAN

Abstract

The mangosteen fruit peel (Garcinia mangostana L.) are used as anti-infl ammatory, antihistamine, treatment of heart disease, antibacterial, antifungal, it is also used for the treatment or therapy of cancer, because it has a cytotoxic activity against cancer cells. Most of Indonesian people have used the mangosteen fruit peel and they produce mangosteen peel into powder or herbal medicine industry has produced its extract in capsules. Efforts to preserve dried mangosteen rind has done by heating process or by gamma irradiation technique. This study aimed to study the effect of gamma irradiation on cytotoxicity activity of extracts and active fractions mangosteen fruit peel against L1210 leukemia cells and chromatogram profi le of extracts and active fractions of the mangosteen fruit peel. Dry powder mangosteen rind irradiated using gamma irradiation dose of 5; 7.5; 10; and 15 kGy. Then each sample was successive macerated in n-hexane, ethyl acetate, and ethanol. Each extract was tested cytotoxicity against L1210 leukemia cells. The ethyl acetate extract was most active extract (IC50 = 4.17 mg/mL) compared with n-hexane extract (IC50 = 8.29 mg/mL) and ethanol extract (IC50 = 7.52 mg/mL). Fractionation of ethyl acetate extract by column chromatography were obtained 6 fractions. The result of cytotoxicity test showed that fraction 1 was the most active fraction (IC50 = 3.97 mg/mL), it was it was still categorized as potential anticancer (IC50 ≤ 20 mg/mL). Profi le chromatogram of fraction 1 with TLC-densitometry showed patches of discoloration on irradiation dose of 10 and 15 kGy. The results of analysis by HPLC fraction 1 showed a decrease of peak area at a dose of 10 kGy was signifi cantly different from the control. Based on the chromatogram profi le of fraction 1 and itscytotoxicity against L1210 leukemia cells, so the maximum dose of 7.5 kGy gamma irradiation can  beap plied on irradiation of mangosteen fruit peel without changing its effi cacy as anti-cancer agent.

References

1. Data statistik export buah manggis dari Indonesia tahun 2010. diambil dari: http://pphp.deptan.go.id/ disp_informasi/1/5/54/1291/potensi_peluang_ekspor_ manggis_indonesia.html. diakses 25 Mei, 2012.

2. Materi pelatihan profesional tanaman obat (Kelas Profesional). Buku 2 Tanaman Obat. Jakarta: Karyasari; 1999. 13-4.

3. Gutierrez-Orozco F and Failla ML. Review biological activities and bioavailability of mangosteen xanthones: a critical review of the current evidence. Nutrients. 2013. 5:3163-83.

4. Lourith N and Kanlayavattanakul M. Biological activity and stability of manggosteen as a potential natural color. Biosci. Biotechnol. Biochem. 2011. 75 (11):2257-59.

5. Januwati M. Peran teknik nuklir dalam argoindustri tanaman obat. Risalah Seminar Ilmiah Aplikasi Isotop Dan Radiasi, Jakarta: BATAN; 2006. 8-27.

6. Winarno H and Katrin E. Benzophenone glucoside isolated from the ethyl acetate extract of the bark of Mahkota Dewa (Phaleria macrocarpa (Scheff.) Boerl.) and its inhibitory activity on leukemia L1210 cell line, Indo. J. Chem. 2009; 8 (1).142-5.

7. Hostettmann K. Assays related to cancer drug discovery. Dalam Buku: Assays for Bioactivity. Volume: 6. Institute of Pharmacognosy and Phytochemistry. University of Lausanne Switzerland. 1991. 84.

8. Winarno EK, Mazda, Rahmawati H, dan Winarno H. Pengaruh iradiasi gamma pada aktivitas sitotoksik daging buah mahkota dewa (Phaleria macrocarpa (Scheff) Boerl.). Jurnal Sains dan Teknologi Nuklir Indonesia Indonesian. 2010. XI (2): 67-76.

9. Charoen S and Aemsiri J. Effects of gamma irradiation on microbiological and chemical quality of herbal teas. Proc. Int’l. Symp. on New Frontier of Irradiated Food and Non-Food Products. KMUTT, Bangkok; 2005.

10. Breitfellner F, Solar S, and Sontag G, Effect of gamma irradiation on fl avonoids in strawberries, European Food Research and Technology. 2002. 215(1): 28-31.

11. Fernando de Melo Vaz A , Pessoa de Souza M , et al. FNS Stability of the antioxidant activity of fl avonoids after gamma irradiation. Food and Nutrition Sciences. 2011. 2: 490-3.

12. Sung NY, Byuna EB, et al. Effect of gamma irradiation on mistletoe (Viscum album) lectin-mediated toxicity and immunomodulatory activity. FEBS Open Bio. 2013. 3: 106–11.
Published
2017-09-30
How to Cite
KATRIN, Ermin; JACOBS, Setiananda; WINARNO, Hendig. Khasiat dan Profil Kromatogram Fraksi Aktif dari Ekstrak Kulit Buah Manggis (Garcinia mangostana L.) yang Diiradiasi. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 15, n. 2, p. 160-166, sep. 2017. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/509>. Date accessed: 24 nov. 2024. doi: https://doi.org/10.35814/jifi.v15i2.509.
Section
Articles