Aktivitas Antibakteri Ekstrak Etil Asetat Media Kultur Fungi Endofi t Nigrospora oryzae dari Rhizophora mucronata
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https://doi.org/10.35814/jifi.v15i2.516
Abstrak
Tujuan penelitian ini adalah untuk memberikan informasi mengenai sifat antibakteri ekstraketil asetat media kultur fungi endofi t daun bakau (R. mucronata).Fungi endofit yang telah diisolasi dari daun R. mucronata diidentifikasi secara makroskopik, mikroskopik, dan molekuler. Ekstrak etil asetat dari media kultur fungi endofi t diuji aktivitas antibakterinya pada Staphylococcus aureus dan Escherichia coli menggunakan metode mikrodilusiuntuk mendapatkan nilai Konsentrasi Hambat Minimum (KHM). Hasil penelitian diperoleh isolat fungi endofi t yaitu Nigrospora oryzae. Ekstrak etil asetat (EtOAc) media kultur fungi endofi t yang diujikan pada E. coli menunjukkan nilai KHM 250 μg/mL terhadap bakteri E. coli dan 500 μg/mL terhadap bakteri S. aureus.
Referensi
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14. Tarman K, Safi tri D, Setyaningsih I, et al.Endophytic fungi isolated from Rhizophora mucronata and their antibacterial activity terhadap bakteri penyebab diare. Squalen Bul Marine & Fisheries Post Biotechnol. 2013. 8(2):69-76.
15. Buatong J, Phongpaichit S, Rukachaisirikul V, Sakayaroj J, et al. Antimicrobial activity of crude extracts from mangrove fungal endophytes. World J Microbiol Biotechnol.2011. 27:3005–3008.
16. White TJ, Bruns TD, Lee SB amdTaylor, JW. Amplifi cation and direct sequencing of fungal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (eds). PCR protocols. Academic San Diego. 1990. p. 315-22.
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22. DomschKH, GamsW, Anderson TH. Compendium of soil fungi volume 1. Eching. 1993. p. 515-6.
23. Prihanto AA, Firdaus M,Nurdiani R, et al. Endophytic fungi isolated from mangrove (Rhizophora mucronata) and its antibacterial activity on Staphylococcus aureus and Escherichia coli. J Food Sci Eng. 2011. 1:386-9.
24. Suciatmih. Diversitas jamur endofi t pada tumbuhan mangrove di Pantai Sampiran dan Pulau Bunaken, Sulawesi Utara. Pros Sem Nas Masy Biodiv Indon, April, 2015: 1(2):177-83.
25. Holetz FB, Pessini GL, Sanches, NR, Cortez DAG, Nakamura GV, Filho BPD,et al.Screening of some plants used in the Brazilian folk medicine for the treatment of infectious disease.Mem Inst Oswaldo Cruz.2002. 97(7):1027-31.
26. Pawle G, Singh SK,et al. Antimicrobial, antioxidant activity and phytocehemical analysis of an endophytic species of Nigrospora isolated from living fossil Ginkgo biloba.Curr Res EnvironAppl Mycol.2014.4(1):1–9.
2. Manilal A, Merdekios B, Idhayadhulla A, Muthukumar C,Melkie M, et al.An in vitro antagonistic efficacy validation of Rhizophora mucronata.Asian Pac J Trop Dis2015. 5(1):28-32.
3. Rahman MdA, Hasan SN, Sampad KS, Das AK, et al. Antinociceptive, antidiarrhoeal and cytotoxic activities of Rhizophora mucronata Lamk. Pharmacologyonline. 2011.1:921-9.
4. Sahoo G, Mulla NSS, AnsariZA, Mohandass C, et al. Antibacterial activity of mangrove leaf extracts against human pathogens. Indian J Pharm Sci. 2012.74(4):348-51.
5. Tarman K, Purwaningsih S, Negara AAAPP, et al. Aktivitas antibakteri ekstrak daun bakau hitam (Rhizophora mucronata) terhadap bakteri penyebab diare. JPHPI. 2013. 16(3):249-58.
6. Hilmi E, Parengrengi, et al.Strategi konservasi mangrove dalam mengurangi dampak bencana di pesisir.Jurnal Pembangunan Pedesaan.2012. 12(2):70-9.
7. Sumampouw M, Bara R, Awaloei H, Posangi, J, et al. Uji efek antibakteri jamur endofit akar bakau Rhizophora stylosa terhadap bakteri Staphylococcus aureus dan Escherichia coli. Jurnal e-Biomedik. 2014. 2(1):1-5.
8. Jalgaonwala RE, Mohite BV, Mahajan RT, et al. A review: natural products from plant associated endophytic fungi.JMicrobiol Biotech Res.2011.1(2):21-32.
9. Kumala S, Agustina E, Wahyudi P, et al.Uji aktivitas antimikroba metabolit sekunder kapang endofit tumbuhan trengguli (Cassia fistula L). Jurnal Bahan Alam Indonesia.2007. 6(2):46-8.
10. Stone JK, Bacon CW and White Jr JF. An overview of endophytic microbes: Endophytism defined. In:Microbial Endophytes. Marcel Dekker:2000. p: 3–29.
11. Petrini O, Sieber TN, Toti L,Viret D, et al. Ecology metabolite production and substrate utilization in endophytic fungi.Nat Toxin.1992.1:185-96.
12. Aly AH, Debbab A,Proksch P, et al. Fungal endophytes: unique plant inhabitants with great promises. Appl Microbiol Biotechnol.2011. 90:1829-45.
13. XuJ, Sherif SE, Peter P, et al. Pestaliopsis a highly creative genus: Chemistry and bioactivity of secondary metabolites. Fungal Divers.2010. 44:15-31.
14. Tarman K, Safi tri D, Setyaningsih I, et al.Endophytic fungi isolated from Rhizophora mucronata and their antibacterial activity terhadap bakteri penyebab diare. Squalen Bul Marine & Fisheries Post Biotechnol. 2013. 8(2):69-76.
15. Buatong J, Phongpaichit S, Rukachaisirikul V, Sakayaroj J, et al. Antimicrobial activity of crude extracts from mangrove fungal endophytes. World J Microbiol Biotechnol.2011. 27:3005–3008.
16. White TJ, Bruns TD, Lee SB amdTaylor, JW. Amplifi cation and direct sequencing of fungal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (eds). PCR protocols. Academic San Diego. 1990. p. 315-22.
17. O`Donnell, K. Fusarium and its near relatives. In: Reynolds, D.R. & Taylor, J.W. (eds). The fungal holomorph: Mitotic, meiotic, and pleomorphic specification in fungal systematics. CAB International. Wallingford.1993. p. 225-33.
18. Hiraishi A, KamagataY, Nakamura N, et al.. Polymerase chain reaction amplification and restriction fragment length polymorphism analysis of 16S rRNA genes from methanogens.JFerment Bioeng.1995. 79: 523-9.
19. CLSI (Clinical and Laboratory Standards Institute).2012. Methods for dilution antimicrobial susceptibility test for bacteria that grow aerobically: Approved standard (9th ed.). CLSI Document M07-A9. USA: Wayne PA.
20. Pratiwi ST. Mikrobiologi Farmasi. Jakarta. Erlangga: 2008. p. 34-53.
21. Kumala S. Mikroba Endofit.. Jakarta.ISFI Penerbitan:2014.p. 25-47.
22. DomschKH, GamsW, Anderson TH. Compendium of soil fungi volume 1. Eching. 1993. p. 515-6.
23. Prihanto AA, Firdaus M,Nurdiani R, et al. Endophytic fungi isolated from mangrove (Rhizophora mucronata) and its antibacterial activity on Staphylococcus aureus and Escherichia coli. J Food Sci Eng. 2011. 1:386-9.
24. Suciatmih. Diversitas jamur endofi t pada tumbuhan mangrove di Pantai Sampiran dan Pulau Bunaken, Sulawesi Utara. Pros Sem Nas Masy Biodiv Indon, April, 2015: 1(2):177-83.
25. Holetz FB, Pessini GL, Sanches, NR, Cortez DAG, Nakamura GV, Filho BPD,et al.Screening of some plants used in the Brazilian folk medicine for the treatment of infectious disease.Mem Inst Oswaldo Cruz.2002. 97(7):1027-31.
26. Pawle G, Singh SK,et al. Antimicrobial, antioxidant activity and phytocehemical analysis of an endophytic species of Nigrospora isolated from living fossil Ginkgo biloba.Curr Res EnvironAppl Mycol.2014.4(1):1–9.
Diterbitkan
2017-09-30
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FAREZA, Muhamad S.; AYOESTY, Lingling T.; WARGIYANTI, Siti R..
Aktivitas Antibakteri Ekstrak Etil Asetat Media Kultur Fungi Endofi t Nigrospora oryzae dari Rhizophora mucronata.
JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 15, n. 2, p. 191-195, sep. 2017.
ISSN 2614-6495.
Tersedia pada: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/516>. Tanggal Akses: 18 sep. 2025
doi: https://doi.org/10.35814/jifi.v15i2.516.
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