Antibacterial Activity of Ethyl Acetate Culture Broth Extract from Endophytic Fungi of Nigrospora oryzae Associated with Rhizophora mucronata
Abstract
The purpose of this study was to provide information on the antibacterial activity of ethyl acetate culture broth extract of endophytic fungi associated with Rhizophora mucronata. An isolated fungi was identifi ed as Nigrospora oryzae using microscopic and molecular analysis. Theantibacterial activity was carried out using micro dilution method to get MIC value. The ethyl acetate extract showed antibacterial activity with MIC value of 250 μg/mL against and Escherichia coli and 500 μg/mL against Staphylococcus aureus.
References
1. BandaranayakeWM. Bioactivities, bioactive compounds and chemical constituents of mangrove plants. WetlEcol Manage2002.10:421-52.
2. Manilal A, Merdekios B, Idhayadhulla A, Muthukumar C,Melkie M, et al.An in vitro antagonistic efficacy validation of Rhizophora mucronata.Asian Pac J Trop Dis2015. 5(1):28-32.
3. Rahman MdA, Hasan SN, Sampad KS, Das AK, et al. Antinociceptive, antidiarrhoeal and cytotoxic activities of Rhizophora mucronata Lamk. Pharmacologyonline. 2011.1:921-9.
4. Sahoo G, Mulla NSS, AnsariZA, Mohandass C, et al. Antibacterial activity of mangrove leaf extracts against human pathogens. Indian J Pharm Sci. 2012.74(4):348-51.
5. Tarman K, Purwaningsih S, Negara AAAPP, et al. Aktivitas antibakteri ekstrak daun bakau hitam (Rhizophora mucronata) terhadap bakteri penyebab diare. JPHPI. 2013. 16(3):249-58.
6. Hilmi E, Parengrengi, et al.Strategi konservasi mangrove dalam mengurangi dampak bencana di pesisir.Jurnal Pembangunan Pedesaan.2012. 12(2):70-9.
7. Sumampouw M, Bara R, Awaloei H, Posangi, J, et al. Uji efek antibakteri jamur endofit akar bakau Rhizophora stylosa terhadap bakteri Staphylococcus aureus dan Escherichia coli. Jurnal e-Biomedik. 2014. 2(1):1-5.
8. Jalgaonwala RE, Mohite BV, Mahajan RT, et al. A review: natural products from plant associated endophytic fungi.JMicrobiol Biotech Res.2011.1(2):21-32.
9. Kumala S, Agustina E, Wahyudi P, et al.Uji aktivitas antimikroba metabolit sekunder kapang endofit tumbuhan trengguli (Cassia fistula L). Jurnal Bahan Alam Indonesia.2007. 6(2):46-8.
10. Stone JK, Bacon CW and White Jr JF. An overview of endophytic microbes: Endophytism defined. In:Microbial Endophytes. Marcel Dekker:2000. p: 3–29.
11. Petrini O, Sieber TN, Toti L,Viret D, et al. Ecology metabolite production and substrate utilization in endophytic fungi.Nat Toxin.1992.1:185-96.
12. Aly AH, Debbab A,Proksch P, et al. Fungal endophytes: unique plant inhabitants with great promises. Appl Microbiol Biotechnol.2011. 90:1829-45.
13. XuJ, Sherif SE, Peter P, et al. Pestaliopsis a highly creative genus: Chemistry and bioactivity of secondary metabolites. Fungal Divers.2010. 44:15-31.
14. Tarman K, Safi tri D, Setyaningsih I, et al.Endophytic fungi isolated from Rhizophora mucronata and their antibacterial activity terhadap bakteri penyebab diare. Squalen Bul Marine & Fisheries Post Biotechnol. 2013. 8(2):69-76.
15. Buatong J, Phongpaichit S, Rukachaisirikul V, Sakayaroj J, et al. Antimicrobial activity of crude extracts from mangrove fungal endophytes. World J Microbiol Biotechnol.2011. 27:3005–3008.
16. White TJ, Bruns TD, Lee SB amdTaylor, JW. Amplifi cation and direct sequencing of fungal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (eds). PCR protocols. Academic San Diego. 1990. p. 315-22.
17. O`Donnell, K. Fusarium and its near relatives. In: Reynolds, D.R. & Taylor, J.W. (eds). The fungal holomorph: Mitotic, meiotic, and pleomorphic specification in fungal systematics. CAB International. Wallingford.1993. p. 225-33.
18. Hiraishi A, KamagataY, Nakamura N, et al.. Polymerase chain reaction amplification and restriction fragment length polymorphism analysis of 16S rRNA genes from methanogens.JFerment Bioeng.1995. 79: 523-9.
19. CLSI (Clinical and Laboratory Standards Institute).2012. Methods for dilution antimicrobial susceptibility test for bacteria that grow aerobically: Approved standard (9th ed.). CLSI Document M07-A9. USA: Wayne PA.
20. Pratiwi ST. Mikrobiologi Farmasi. Jakarta. Erlangga: 2008. p. 34-53.
21. Kumala S. Mikroba Endofit.. Jakarta.ISFI Penerbitan:2014.p. 25-47.
22. DomschKH, GamsW, Anderson TH. Compendium of soil fungi volume 1. Eching. 1993. p. 515-6.
23. Prihanto AA, Firdaus M,Nurdiani R, et al. Endophytic fungi isolated from mangrove (Rhizophora mucronata) and its antibacterial activity on Staphylococcus aureus and Escherichia coli. J Food Sci Eng. 2011. 1:386-9.
24. Suciatmih. Diversitas jamur endofi t pada tumbuhan mangrove di Pantai Sampiran dan Pulau Bunaken, Sulawesi Utara. Pros Sem Nas Masy Biodiv Indon, April, 2015: 1(2):177-83.
25. Holetz FB, Pessini GL, Sanches, NR, Cortez DAG, Nakamura GV, Filho BPD,et al.Screening of some plants used in the Brazilian folk medicine for the treatment of infectious disease.Mem Inst Oswaldo Cruz.2002. 97(7):1027-31.
26. Pawle G, Singh SK,et al. Antimicrobial, antioxidant activity and phytocehemical analysis of an endophytic species of Nigrospora isolated from living fossil Ginkgo biloba.Curr Res EnvironAppl Mycol.2014.4(1):1–9.
2. Manilal A, Merdekios B, Idhayadhulla A, Muthukumar C,Melkie M, et al.An in vitro antagonistic efficacy validation of Rhizophora mucronata.Asian Pac J Trop Dis2015. 5(1):28-32.
3. Rahman MdA, Hasan SN, Sampad KS, Das AK, et al. Antinociceptive, antidiarrhoeal and cytotoxic activities of Rhizophora mucronata Lamk. Pharmacologyonline. 2011.1:921-9.
4. Sahoo G, Mulla NSS, AnsariZA, Mohandass C, et al. Antibacterial activity of mangrove leaf extracts against human pathogens. Indian J Pharm Sci. 2012.74(4):348-51.
5. Tarman K, Purwaningsih S, Negara AAAPP, et al. Aktivitas antibakteri ekstrak daun bakau hitam (Rhizophora mucronata) terhadap bakteri penyebab diare. JPHPI. 2013. 16(3):249-58.
6. Hilmi E, Parengrengi, et al.Strategi konservasi mangrove dalam mengurangi dampak bencana di pesisir.Jurnal Pembangunan Pedesaan.2012. 12(2):70-9.
7. Sumampouw M, Bara R, Awaloei H, Posangi, J, et al. Uji efek antibakteri jamur endofit akar bakau Rhizophora stylosa terhadap bakteri Staphylococcus aureus dan Escherichia coli. Jurnal e-Biomedik. 2014. 2(1):1-5.
8. Jalgaonwala RE, Mohite BV, Mahajan RT, et al. A review: natural products from plant associated endophytic fungi.JMicrobiol Biotech Res.2011.1(2):21-32.
9. Kumala S, Agustina E, Wahyudi P, et al.Uji aktivitas antimikroba metabolit sekunder kapang endofit tumbuhan trengguli (Cassia fistula L). Jurnal Bahan Alam Indonesia.2007. 6(2):46-8.
10. Stone JK, Bacon CW and White Jr JF. An overview of endophytic microbes: Endophytism defined. In:Microbial Endophytes. Marcel Dekker:2000. p: 3–29.
11. Petrini O, Sieber TN, Toti L,Viret D, et al. Ecology metabolite production and substrate utilization in endophytic fungi.Nat Toxin.1992.1:185-96.
12. Aly AH, Debbab A,Proksch P, et al. Fungal endophytes: unique plant inhabitants with great promises. Appl Microbiol Biotechnol.2011. 90:1829-45.
13. XuJ, Sherif SE, Peter P, et al. Pestaliopsis a highly creative genus: Chemistry and bioactivity of secondary metabolites. Fungal Divers.2010. 44:15-31.
14. Tarman K, Safi tri D, Setyaningsih I, et al.Endophytic fungi isolated from Rhizophora mucronata and their antibacterial activity terhadap bakteri penyebab diare. Squalen Bul Marine & Fisheries Post Biotechnol. 2013. 8(2):69-76.
15. Buatong J, Phongpaichit S, Rukachaisirikul V, Sakayaroj J, et al. Antimicrobial activity of crude extracts from mangrove fungal endophytes. World J Microbiol Biotechnol.2011. 27:3005–3008.
16. White TJ, Bruns TD, Lee SB amdTaylor, JW. Amplifi cation and direct sequencing of fungal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (eds). PCR protocols. Academic San Diego. 1990. p. 315-22.
17. O`Donnell, K. Fusarium and its near relatives. In: Reynolds, D.R. & Taylor, J.W. (eds). The fungal holomorph: Mitotic, meiotic, and pleomorphic specification in fungal systematics. CAB International. Wallingford.1993. p. 225-33.
18. Hiraishi A, KamagataY, Nakamura N, et al.. Polymerase chain reaction amplification and restriction fragment length polymorphism analysis of 16S rRNA genes from methanogens.JFerment Bioeng.1995. 79: 523-9.
19. CLSI (Clinical and Laboratory Standards Institute).2012. Methods for dilution antimicrobial susceptibility test for bacteria that grow aerobically: Approved standard (9th ed.). CLSI Document M07-A9. USA: Wayne PA.
20. Pratiwi ST. Mikrobiologi Farmasi. Jakarta. Erlangga: 2008. p. 34-53.
21. Kumala S. Mikroba Endofit.. Jakarta.ISFI Penerbitan:2014.p. 25-47.
22. DomschKH, GamsW, Anderson TH. Compendium of soil fungi volume 1. Eching. 1993. p. 515-6.
23. Prihanto AA, Firdaus M,Nurdiani R, et al. Endophytic fungi isolated from mangrove (Rhizophora mucronata) and its antibacterial activity on Staphylococcus aureus and Escherichia coli. J Food Sci Eng. 2011. 1:386-9.
24. Suciatmih. Diversitas jamur endofi t pada tumbuhan mangrove di Pantai Sampiran dan Pulau Bunaken, Sulawesi Utara. Pros Sem Nas Masy Biodiv Indon, April, 2015: 1(2):177-83.
25. Holetz FB, Pessini GL, Sanches, NR, Cortez DAG, Nakamura GV, Filho BPD,et al.Screening of some plants used in the Brazilian folk medicine for the treatment of infectious disease.Mem Inst Oswaldo Cruz.2002. 97(7):1027-31.
26. Pawle G, Singh SK,et al. Antimicrobial, antioxidant activity and phytocehemical analysis of an endophytic species of Nigrospora isolated from living fossil Ginkgo biloba.Curr Res EnvironAppl Mycol.2014.4(1):1–9.
Published
2017-09-30
How to Cite
FAREZA, Muhamad S.; AYOESTY, Lingling T.; WARGIYANTI, Siti R..
Antibacterial Activity of Ethyl Acetate Culture Broth Extract from Endophytic Fungi of Nigrospora oryzae Associated with Rhizophora mucronata.
JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 15, n. 2, p. 191-195, sep. 2017.
ISSN 2614-6495.
Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/516>. Date accessed: 16 apr. 2024.
doi: https://doi.org/10.35814/jifi.v15i2.516.
Issue
Section
Articles
License
Licencing
All articles in Jurnal Ilmu Kefarmasian Indonesia are an open-access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which permits unrestricted non-commercial used, distribution and reproduction in any medium.
This licence applies to Author(s) and Public Reader means that the users mays :
- SHARE:
copy and redistribute the article in any medium or format - ADAPT:
remix, transform, and build upon the article (eg.: to produce a new research work and, possibly, a new publication) - ALIKE:
If you remix, transform, or build upon the article, you must distribute your contributions under the same license as the original. - NO ADDITIONAL RESTRICTIONS:
You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
It does however mean that when you use it you must:
- ATTRIBUTION: You must give appropriate credit to both the Author(s) and the journal, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
You may not:
- NONCOMMERCIAL: You may not use the article for commercial purposes.
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Tools
