The Hepatoprotective Effect of Ethanol Extract of Syzygium campanulatum (Korth) and Syzygium aromaticum (L) Leaf on Male Wistar Rat (Rattus Norvegicus) was Induced by Paracetamol

  • Much Ilham Novalisa Aji Wibowo UNIVERSITAS MUHAMADIYAH PURWOKERTO
  • Nur Aeni UNIVERSITAS MUHAMADIYAH PURWOKERTO
  • Zidna Mazayatul Huda UNIVERSITAS MUHAMADIYAH PURWOKERTO
  • Nunuk Aries Nurulita UNIVERSITAS MUHAMADIYAH PURWOKERTO
DOI: https://doi.org/10.35814/jifi.v15i2.517

Abstract

Syzygium campanulatum and Syzygium aromaticum contains antioxidant components suchas flavonoids, phenolic, and terpenoids. May have hepatoprotective properties in reducing SGPT and SGOT activity. This research wants to determine the potency of hepatoprotective of ethanolic extract of Syzygium campanulatum (Korth) and Syzygium aromaticum leaf compared with curcuma tablets. This research uses 24 male Wistar rats divided into 6 groups: I, II, III (as a normal, induction, and compared control), group IV, V, VI were treated 105, 210, and 420 mg/kg BW respectively. The study was conducted for 9 days. After 7 days of treatment, treated groups were exposed by hepatotoxic dose of paracetamol (2000 mg/kg BW). The SGPT and SGOT activity of all groups was measured by enzimatic assay. The result can be concluded that Syzygium campanulatum extract was found to be active as hepatoprotective agent with 210 mg/kg BW dosage (SGPT 21.76 ± 3.98 U/L and SGOT 7.32±6.74U/L) as eff ective as with the curcuma tablets (SGPT 23.91 ± 4.41 U/L and SGOT 14.12±5.37 U/L) and the hepatoprotective activity of Syzygium campanulatum extract at a dosage 420 mg/kg BW better than curcuma tablets (SGPT 12.43 ± 6.51 U/L and SGOT 6.64 ± 5.88 U/L). While the hepatoprotec Syzygium campanulatum and Syzygium aromaticum contains antioxidant components such as flavonoids, phenolic, and terpenoids.May have hepatoprotective properties in reducing SGPT and SGOT activity. This research wants to determine the potency of hepatoprotective of ethanolic extract of Syzygium campanulatum (Korth) and Syzygium aromaticum leaf compared with curcuma tablets. This research uses 24 male Wistar rats divided into 6 groups: I, II, III (as a normal, induction, and compared control), group IV, V, VI were treated 105, 210, and 420 mg/kg BW respectively. The study was conducted for 9 days. After 7 days of treatment, treated groups were exposed by hepatotoxic dose of paracetamol (2000 mg/kg BW). The SGPT and SGOT activity of all groups was measured by enzimatic assay. The result can be concluded that Syzygium campanulatum extract was found to be active as hepatoprotective agent with 210 mg/kg BW dosage (SGPT 21.76 ± 3.98 U/L and SGOT 7.32±6.74U/L) as eff ective as with the curcuma tablets (SGPT 23.91 ± 4.41 U/L and SGOT 14.12±5.37 U/L) and the hepatoprotective activity of Syzygium campanulatum extract at a dosage 420 mg/kg BW better than curcuma tablets (SGPT 12.43 ± 6.51 U/L and SGOT 6.64 ± 5.88 U/L). While the hepatoprotective activity of Syzygium aromaticum extracts eff ective as with curcuma tablets at all dosage variation.

References

1. Tappi. et al. 2013. “Gambaran Histopatologi Hati Tikus Wistar yang Diberikan Jus Tomat (Solanum lycopersicum) Pasca Kerusakan Hati Wistar yang Diinduksi Karbon Tetraklorida (CCl4)”.Jurnal e- Biomedik (eBM) 1 (3).

2. Yenny, Herwana E., Marwoto, W., Setiabudy, R.. 2011. Efek Schizandrinec terhadap kerusakan hati akibat pemberian parasetamol pada tikus. Universa Medika 24 (4) : 161-6

3. Amirudin, R., 2009. Fisiologi dan Biokimia hati : Buku Ajar Ilmu Penyakit Dalam Edisi V. Jakarta. Interna Publishing. Hal : 627.

4. Wijayanti. 2008. Efek Hepatoprotektif Ekstrak Etanol 70% Daun Salam (Syzygium polyanthum) Pada Tikus Putih jantan yang di Induksi CCl4 [skripsi]. Surakarta: Fakultas Farmasi Universitas Muhammadiyah Surakarta.

5. Yu W. G . , Q i a n J i e . , L u Ya n . H u a . 2 0 11. “Hepatoprotective effects of 2’,4’-dihydroxy- 6’methoxy3’,5’dimethylchalcone on CCl4-induced acute liver injury in mice”.Journal of Agricultural and Food Chemistry,59, (2).Availablefrom: http:// pubs.acs.org/doi/abs/10.1021/jf2042032. [Accessed: 22nd July 2016]

6. Yi, Jine. et al. 2014. Betulinic acid prevents alcoholinduced liver damage by improving theantioxidant system in mice. Journal of Veterinary Science. 15 (1), 141-8

7. Mu’nisa, A., Wresdiyati, T., Kusumorini, N., Manalu, W. 2012. Aktivitas Antioksidan Ekstrak Daun Cengkeh. Jurnal Veteriner. 13 (3). Hal 272 – 277.15 (1), 141-8

8. Nirmala,K Girija, K Lakshman, and T Divya. 2012. Hepatoprotective activity of Musa paradisiaca on experimental animal models. Asian Pac J Trop Biomed 2012 Jan; 2(1): 11–5.

9. 9. M e m o n , A . H . e t a l. 2 0 14 . “ Is o l a t i o n , Characterzation, Crystal Structure Elucidation, and Anticancer Study of Dimethyl Cardamonin Isolated From Syzygiumcampanulatum. Published by: Hindawi Publishing Corporation.

10. Lumingkewas, M., Manarisip, J., Indriaty, F., Walangitan., A., Mandei, J., Suryanto, E. (2014). Aktivitas Antifotooksidan dan komposisi fenolik dari daun cengkeh (Eugenia aromaticum). ChemProg. 7 (2). Hal 96 – 104.

11. Anas, Yance ., Niken Puspitasari., Maulita Cut Nuria. 2013. Aktivitas Stimulansia Ekstrak Etanol Bunga Dan Daun Cengkeh (syzygium aromaticum (L.) Merr.& Perry.) pada Mencit Jantan Galur Swiss Beserta Identifikasi Golongan Senyawa Aktifnya [skripsi]. Semarang: Fakultas Farmasi Universitas Wahid Hasyim Semarang.

12. Dahlan, Sopiyudin.2011. Statistik Untuk Kedokteran dan Kesehatan Edisi 5. Jakarta, Salemba Medika.

13. Ogata M, Hoshi M, Urano S, Endo T (2000). Antioxidant activity of eugenol and related monomeric and dimeric compounds. Chem. Pharm. Bull. 48: 1467-9.

14. Agnieszka, S.C., Karolina, Pilipów, Martyna K.Z. 2010. Protective effect of betulin and betulinicacid on acetaminophen andethanol-induced cytotoxicity and reactiveoxygen species production in HepG2 cells. Journal of Pre- Clinical and Clinical Research.4 (2), 096-100.
Published
2017-09-30
How to Cite
WIBOWO, Much Ilham Novalisa Aji et al. The Hepatoprotective Effect of Ethanol Extract of Syzygium campanulatum (Korth) and Syzygium aromaticum (L) Leaf on Male Wistar Rat (Rattus Norvegicus) was Induced by Paracetamol. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 15, n. 2, p. 196-202, sep. 2017. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/517>. Date accessed: 19 apr. 2024. doi: https://doi.org/10.35814/jifi.v15i2.517.
Citation Formats
Issue
Vol 15 No 2 (2017): JIFI
Section
Articles
License

Licencing
All articles in Jurnal Ilmu Kefarmasian Indonesia are an open-access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which permits unrestricted non-commercial used, distribution and reproduction in any medium.

This licence applies to Author(s) and Public Reader means that the users mays :

  • SHARE:
    copy and redistribute the article in any medium or format
  • ADAPT:
    remix, transform, and build upon the article (eg.: to produce a new research work and, possibly, a new publication)
  • ALIKE:
    If you remix, transform, or build upon the article, you must distribute your contributions under the same license as the original.
  • NO ADDITIONAL RESTRICTIONS:
    You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

It does however mean that when you use it you must:

  • ATTRIBUTION: You must give appropriate credit to both the Author(s) and the journal, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.

You may not:

  • NONCOMMERCIAL: You may not use the article for commercial purposes.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.