Antibacterial Actifity of Etanol Extract From Leaves and Bark of Sapodilla (Manilkara zapota (L.) Van Royen)
Abstract
Sapodilla (Manilkara zapota (L.) van Royen) is a plant that grows in a tropical climate, fruit all year round and the distribution is in the widespread in Indonesia. The contents of secondary metabolites in the leaves and the bark sapodilla are flavonoids, phenols and saponins are known to inhibit the growth of bacteria. The purpose of this research is to know the antibacterial activity of ethanol extract leaves and bark Sapodilla against Staphylococcus aureus and Propionibacterium acnes bacteria. The study was performed by disc diffusion method with the varyation concentrations of ethanol extract from the leaves and bark with a concentration of 50%, 25%, 12.5%, 6.25% and 3.125% respectively and the positive control is clindamycin and the negative control is DMSO . The diameter of the inhibition zone formed on the ethanol extract test of the leaves sapodilla against Staphylococcus aureus and Propionibacterium acnes at a concentration of 50% was 14.18 ± 0.13 mm and 15.33 ± 0.25 mm, respectively. While the diameter of the inhibition zone formed on the ethanol extract test of the bark against Staphylococcus aureus and Propionibacterium acnes at a concentration of 50% was 14.22±0.15 mm and 18.30±0.23 mm, respectively. The results of the data statistical analysis using oneway ANOVA, it shown a significant difference (p < 0.05) between the variation of the concentration of the extract. The test results shown that the antibacterial activity ethanol extract of the leaves and bark of Sapodilla had activity for inhibit the growth of Staphylococcus aureus and Propionibacterium acnes bacteria.
References
2. Milind P and Preeti. Chickoo: a wonderful gift from nature. Int J. Res Ayurveda Pharm. 2015. 6(4):544-50.
3. Bhargavi S, Buthapalli K, Dantu KS, Buchiraju R, Sreekanth N. An evaluation of the antibacterial activity of root extracts of Manilkara zapota against Staphylococcus aureus and eschericha coli. International Journal Of Phytopharmacology. 2013. 4(3):171-3.
4. Ganguly A and Abdur R. Evaluation of the cytotoxic, antimicrobial, antioxidant, anthelmintic and CNS
depressant activities of Manilkara zapota leaf (Sapotaceae). World Journal Of Pharmaceutical
Research. 2014. 4(1):272-83.
5. Osman MA, Abdul A, Rowshanul H and Rezaul K. Antimicrobial investigation on Manilkara zapota (L.)
P. Royen. International Journal Of Drug Development And Research. 2011. 3(1):185-90.
6. Djamil R dan Zaidan S. Isolasi senyawa flavonoid dari ekstrak metanol daun katuk (Sauropus androgynus
(L.) Merr), Euphorbiaceae. Jurnal Ilmu Kefarmasian Indonesia. 2016. 14(1):57-61.
7. Ratna YRD, Ardani US, Fathiana Z, Rahmatillah A dan Trisharyanti I. Daya antibakteri ekstrak dan fraksifraksi
daun jambu mete (anacardium occientale L.) terhadap bakteri staphylococcus aureus sensitif dan multiresisten. Jurnal Ilmu Kefarmasian Indonesia. 2016. 14(1):103-10.
8. Movita T. Acnes vulgaris. Continuing Medical Education. 2013. 40(4):269-22.
9. Afriyanti RN. Acne vulgaris pada remaja. J Majority. 2015. 4(6):102-9.
10. Identifi kasi senyawa aktif antibakteri dengan metode bioautogravi KLT terhadap ekstrak etanol tangkai
daun alas (Colocasia esculenta (L.) Schott. Prosiding Penelitian Spesia Unisba, Bandung 18-19 Agustus, 2015:583-90.
11. Nazri NA, Ahmat A, Adnan SA, Syed M, Syaripah R. In vitro antibacterial and radical scavenging activities of Malaysian table salad. African Journal Of Biotechnology. 2011. 10(30):5728-35.
12. Cowan MM. Plant products as antimicrobial agents. Clinical Microbiology Reviews. 1999. 12(4): 564-82.
13. Nuria MC, Faizatun A, Sumantri. Uji antibakteri ekstrak etanol daun jarak pagar (Jatropha cuircas L)
terhadap bakteri Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, dan salmonella typhi
TCC 1408. Jurnal Ilmu-ilmu Pertanian. 2009. 5(2): 26-37.
14. Cushnie TPT, Lamb AJ. Antimicrobial activity of flavonoids. International Journal of Antimicrobial
Agents. 2005. 26:343-56.
15. Lorent JH, Quetin-Leclerq J, Mingeot-Leclercq MP. The amphiphilic nature of saponins and their effects
on artificial and biological membranes and potential consequences for red blood and cancer cells. Org
Biomol Chem. 2014. 12(44):8803-22.
Licencing
All articles in Jurnal Ilmu Kefarmasian Indonesia are an open-access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which permits unrestricted non-commercial used, distribution and reproduction in any medium.
This licence applies to Author(s) and Public Reader means that the users mays :
- SHARE:
copy and redistribute the article in any medium or format - ADAPT:
remix, transform, and build upon the article (eg.: to produce a new research work and, possibly, a new publication) - ALIKE:
If you remix, transform, or build upon the article, you must distribute your contributions under the same license as the original. - NO ADDITIONAL RESTRICTIONS:
You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
It does however mean that when you use it you must:
- ATTRIBUTION: You must give appropriate credit to both the Author(s) and the journal, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
You may not:
- NONCOMMERCIAL: You may not use the article for commercial purposes.
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Tools
