Isolation of β-sitosterol from n-hexane Extracts of Mimba Leaves(Azadirachta indica A. Juss) and α-Glucosidase Enzyme Treatment

  • Ivan Santoso UNIVERSITAS PANCASILA
  • Partomuan Simanjuntak UNIVERSITAS PANCASILA
  • Rahmaniar Rahmaniar UNIVERSITAS PANCASILA
DOI: https://doi.org/10.35814/jifi.v15i2.523

Abstract

Mimba, Azadirachta indica is one of the family Meliaceae plants, which be used to lower blood sugar and contain terpenoids, fl avonoids, alkaloids, saponins, tannins, fatty acids, steroids and triterpenoids. The aim of this research is to isolate and identify one of chemical structure and to know the inhibition α-glucosidase enzyme from extract of leaves Mimba, Azadirachta indica. Extraction is done by maceration using n-hexane, ethyl acetate, methanol, water and then treatment for antidiabetic activity using the inhibition of the enzyme α-glucosidase. Purifi cation of n-hexane extract (the higher activity) is subjected by column chromatography (SiO2; (i).n-hexane-ethyl acetate = 10 : 1 ~ 1 : 1, ethyl acetate, (ii). n-hexane-ethyl acetate = 5 : 1) gave one pure compound as white powder. Identifi cation by interpretation of spectra [UV, IR and NMR (1H, 13C-NMR and DEPT)] the isolate compound is β-sitosterol has inhibion α-glucosidation enzyme as (IC50 208,1 μg/mL).

References

1. Soerianegara, I. dan R. H. M. J. Lemmens. Plant Resourcesof South East Asia. Timber Tres: Major Commercial Timbers. Bogor. 1986; 5 (1).

2. American Diabetes Association. Diagnosis and classifi cation of diabetes melliutus. Diabetes Care; 2012, 35 (1): h 64-71

3. Redaksi Agromedia. Ramuan Tradisional untuk Mengatasi Aneka pada Penyakit. Jakarta: Agromedia Pustaka; 2006, h 153-4.

4. Kim YM, Jeong YK, Wang MH, Lee WY, and Rhee HI. inhibitory effect of pine extract on alpha- glucosidase activity and postprandial hyperglycemia. Nutrition. 2005; 21:756-61.

5. Lee Dong-sun and Lee Sang-Han. Genistein, a Soy Isofl avon, is a Potent α-Glucosidase Inhibitor. FEBS Letters. 2001; 501, 84-6.

6. Li Yuhao, Wen Suping, Prasad, B.K., Peng Gang, Qian G.L., Yamahara Johji and Roufogalis B.D., Punica granatum Flower Extract, a poten α-Glucosidase Inhibitor, Improves Postprandial Hyperglikemia in Zuker Diabetic Fatty Rats. Etnopharm. 2005; 99: 239-44.

7. Ramachandran A, Snehalatha, and Ma RC (2013) Diabetes in South-East Asia: an update for 2013 for the IDF diabetes atlas. Diabetes Res Clin Pract. http://dx.doi.org/10.1016/j. diabres. 2013.11.011ofi ernvjevnejn

8. Nirmala A, Saroja S, Vasanthi HR, Lalitha G. Hypoglycemic effect of Basella rubra in streptozotocin induced diabetic albino rats. Journal of Pharmacognosy and Phytotherapy, 2009; 1: 25-30.

9. Susana B. Genta, Wilfredo M. Cabrera, María I. Mercadob, Alfredo Grauc, César A. Catalánb, Sara S. Sáncheza, Hypoglycemic activity of leaf organic extracts from Smallanthus sonchifolius:Constituents of the most active fractions, Chemico-Biological Interactions 185 (2010) 143– 52.

10. Kardiman A dan Dhalimi A. “MIMBA (Azadirachta indica A.Juss) TANAMAN MULTI MANFAAT”. Perkembangan Teknologi TRO Vol. XV, No. 1, 2003

11. Sudarsono, Gunawan, D., Wahyuono, S., Donatus, A.I., dan Purnomo, 2002, Tumbuhan Obat II, Hasil Penelitian, Sifat-Sifat, dan Penggunaan, Pusat Studi Obat Tradisional UGM, Yogyakarta.

12. Chattopadhyay, R.R. and Bandyopadhyay, M., 2005, Effect of Azadirachta indica Leaf Extract on Serum Lipid Profi le Changes in Normal and Streptozotocin Induced Diabetic Rats, African Journal of Biomedical Research, vol.8, pp.101-4.

13. Biu, A.A., Yusufu, S.D., and Rabo, J.S., 2009, Phytochemical screening of AzadirachtaIndica (Meliaceae) in Maiduguri, Nigeria, Bioscience Research Communications, vol. 21, pp. 6-10.

14. Khan, I., Srikakolupu, S.R., Darsipudi, S., Gotteti, S.D., and Amaranadh, C., 2010, Phytochemical Studies and Screening of Leaves Extracts of Azadirachta indica for its anti-microbial Activity Against Dental Pathogens, Science Research, vol. 2, pp.246-50.

15. Aslam, F., Khalil-ur-Rehman, Asghar, M., and Sarwar. M., 2009, Antibacterial Activity of Various Phytoconstituents of Neem, Pakistan Journal Agricultural Science, vol 46, pp. 3-6.

16. Suirta, I.W, Puspawati, N. M., dan Gumiati, N. K., 2007, Isolasi dan Identifi kasi Senyawa Aktif Larvasida dari Biji Mimba Azadirachta indica A. Juss) terhadap Larva nyamuk Demam Berdarah (Aedes aegypti), Jurnal Kimia,vol.1, hal. 47-54.

17. Rembold H., 1989. The Azadirachtins-their potential for insect control.

18. Prakash VS and Prakash I. Isolation of Stigmasterol and β-sitosterol from the dichloromethane extract pf Rubus suavissimus. Intern. Current Pharm. Journal 2012., 1 (9), p. 29-242.
Published
2017-09-30
How to Cite
SANTOSO, Ivan; SIMANJUNTAK, Partomuan; RAHMANIAR, Rahmaniar. Isolation of β-sitosterol from n-hexane Extracts of Mimba Leaves(Azadirachta indica A. Juss) and α-Glucosidase Enzyme Treatment. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 15, n. 2, p. 223-227, sep. 2017. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/523>. Date accessed: 03 july 2024. doi: https://doi.org/10.35814/jifi.v15i2.523.
Citation Formats
Issue
Vol 15 No 2 (2017): JIFI
Section
Articles
License

Licencing
All articles in Jurnal Ilmu Kefarmasian Indonesia are an open-access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which permits unrestricted non-commercial used, distribution and reproduction in any medium.

This licence applies to Author(s) and Public Reader means that the users mays :

  • SHARE:
    copy and redistribute the article in any medium or format
  • ADAPT:
    remix, transform, and build upon the article (eg.: to produce a new research work and, possibly, a new publication)
  • ALIKE:
    If you remix, transform, or build upon the article, you must distribute your contributions under the same license as the original.
  • NO ADDITIONAL RESTRICTIONS:
    You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

It does however mean that when you use it you must:

  • ATTRIBUTION: You must give appropriate credit to both the Author(s) and the journal, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.

You may not:

  • NONCOMMERCIAL: You may not use the article for commercial purposes.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.