Analgesic Effectivty Of Nanoparticles Chitosan-Ethanol Leaf Extract Papaya (Carica Papaya L.) In White Male Mice (Mus Mucculus)

  • Juliferd Gredi UNIVERSITAS TANJUNGPURA PONTIANAK
  • Wintari Taurina UNIVERSITAS TANJUNGPURA PONTIANAK
  • Mohamad Andrie UNIVERSITAS TANJUNGPURA PONTIANAK
DOI: https://doi.org/10.35814/jifi.v15i2.524

Abstract

Papaya leaves has been shown to have efficacy as an analgesic. Analgesic effect caused by the flavonoids in leaves of papaya. To enhance the analgesic effi cacy papaya into dosage formulatio of nanoparticles. The purpose of this research was to create and characterized nanoparticles from ethanol extract of papaya leaves then tested for effectiveness as an analgesic. This study used polymer chitosan and NaTPP to created nanoparticles through ionic gelation method and analgesic activity tested using writhing test method and statistical tested. Optimal formula of nanoparticles were produced at concentration of 0.2% chitosan, Na-TPP extract 0.1% and 4% with a ratio of 6: 1: 1. The nanoparticles was formed a size of 255.4 nm with polidispers index of 0.7, the zeta potential of + 11,1mV, the adsorption effi ciency by 87% and rounded morphology (spherical). Analgesic effectiveness tested by comparison between the groups ethanol extract of papaya and the groups nanoparticles of chitosan-ethanol extract papaya statistically using the T-test Independent. The results of this research indicate that there are statistically signifi cant differences (p <0.05). This results shows that the preparation nanoparticles of chitosan-ethanol extract papaya is more effective as an analgesic than the ethanol extract of papaya leaves.

References

1. Suryanto, Edi, 2012. Fitokimia Antioksidan. Penerbit Putra Media Nusantara : Surabaya.

2. Gunawan, S.G., Setiabudy, R., Nafrialdi, Elsyabeth, editor. 2008. Farmakologi dan Terapi Edisi 5. FKUI, Jakarta.

3. TAKEUCHI H., YAMAMOTO H., and KAWASHIMA, Y., Advanced Drug Delivery Reiews, 47 (2001) 39-54

4. Agnihotri, S.A., Nadagounda N., Mallikarjuna, Tejraj M., Aminabhavi. 2004. Recent advances on chitosanbased micro- and nanoparticles in drug delivery. J. Control. Release, 100, 5-28 5. FAN W., YAN W., XU Z., NI H., Colloids and Surfaces B: Biointerfaces, 90 (2012) 21-27

6. Dwi, Santi Astuti. Efek Estrak Etanol 70% Daun Pepaya Terhadap aktivitas AST dan ALT Pada Tikus Galur Wistar Setelah Pemberian Obat Tuberkulosis (Isoniazid dan Rifampisin). Skripsi. Fakultas Farmasi Universitas Setia Budi. 2009.

7. Andersen, M. and Markham K.R. Flavonoids: Chemistry, Biochemistry, and Applications. USA: Taylor & Francis Group 2; 2006. Hal 3-4.

8. Yulanda H. Ekstraksi, fraksinasi, dan pencirian pati rimpang temulawak. skripsi. 2007. Bogor: Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor.

9. Mishra M, Kumar H, Singh RK, Tripathi K. 2008. Diabetes and nanomaterials. Digest Journey of Nanomaterials and Biostructures 3: 109-113.

10. Departemen Kesehatan Republik Indonesia. 1989. Materia Medika Indonesia. Jilid V. Jakarta : Departemen Kesehatan Republik Indonesia. Hal: 179; 333-337; 549-553.

11. Markham, K.R. Cara Mengidentifikasi Flavonoid, diterjemahkan oleh Kosasih Padmawinata. Bandung: Penerbit ITB; 1988: 15.

12. Iswandana Raditya, Anwar Effi onora, dan Jufri Mahdi. 2013. Formulasi Nanopartikel Verapamil Hidroklorida dari Kitosan dan Natrium Tripolifosfat dengan Metode Gelasi Ionik. Jurnal Farmasi Indonesia Vol. 6 No. 4.

13. Wu Y, Yang W, Changchun W, Jianhua H and Fu S. 2005. Chitosan nanoparticles as a novel delivery system for ammonium glycyrrhizinate. International Journal of Pharmaceutics.;(295):235-45.

14. Mardliyati E, Sjaikhurrizal El Muttaqien, Damai R Setyawati, Idah Rosidah, Sriningsih. 2012. Preparasi dan Aplikasi Nanopartikel Kitosan sebagai Sistem Penghantaran Insulin secara Oral. Pusat Teknologi Farmasi dan Medika BPPT.

15. Yesi Desmiaty, Julia Ratnawati, Peni Andini.2009. PENENTUAN JUMLAH FLAVONOID TOTAL EKSTRAK ETANOL DAUN BUAH MERAH (PANDANUS CONOIDEUS LAMK.) SECARA KOLORIMETRI KOMPLEMENTER. Seminar Nasional POKJANAS TOI XXXVI Universitas Sanata Dharma, Yogyakarta.

16. Stella Octavianus, Fatimawali dan Widya A. 2014. Lolo.UJI EFEK ANALGETIK EKSTRAK ETANOL DAUN PEPAYA (Carica pepaya L) PADA MENCIT PUTIH JANTAN (Mus mucculus). Jurnal Ilmiah Farmasi – UNSRAT Vol. 3 No. 2.

17. Yulanda H. Ekstraksi, fraksinasi, dan pencirian pati rimpang temulawak. skripsi. 2007. Bogor: Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor.

18. Pankhurst QA, Connolly J, Jones SK, Dobson J. 2003. Applications of magnetic nanoparticles in biomedicine [ulas balik]. J. Phys. D: Appl. Phys 36:167-181.

19. Tiyaboonchai, w., 2003, Chitosan nanoparticles : a promising system for Drug Delivery, naresuan University Journal 2003; 11(3): 51-66.

20. Ram B. Gupta, Uday B. Kompella. Nanoparticle Technology for Drug Delivery. New York: Taylor & Francis Group; 2006.

21. Dounighi. M, Eskandari. R, Avadi. MR, Zolfagharian. H, Sedagi. M.M, Rezayat. M. 2012. Preparation and In Vitro Characterization of Chitosan Nanoparticles Containing Mesobuthus Eupeus Scorpion Venom as an Antigen Delivery System. The journal of Venomous Animals and Toxins Tropical Diseases. Vol 18. 44-52.

22. Dukhovich, F.S., Darkhovskii, M.B., Gorbatova, E.N., Kurochkin, V.K. Molecular Recognition. New York: Pharmacological Aspects. Nova Science Publishers,. 2003.

23. Soheyla H., Foruhe Z,. Effect of Zeta Potential on the Properties of Nano-Drug Delivery Systems. Tropical Journal of Pharmaceutical Research. April 2013; 12 (2): 265-273.
Published
2017-09-30
How to Cite
GREDI, Juliferd; TAURINA, Wintari; ANDRIE, Mohamad. Analgesic Effectivty Of Nanoparticles Chitosan-Ethanol Leaf Extract Papaya (Carica Papaya L.) In White Male Mice (Mus Mucculus). JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 15, n. 2, p. 228-235, sep. 2017. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/524>. Date accessed: 27 apr. 2024. doi: https://doi.org/10.35814/jifi.v15i2.524.
Citation Formats
Issue
Vol 15 No 2 (2017): JIFI
Section
Articles
License

Licencing
All articles in Jurnal Ilmu Kefarmasian Indonesia are an open-access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which permits unrestricted non-commercial used, distribution and reproduction in any medium.

This licence applies to Author(s) and Public Reader means that the users mays :

  • SHARE:
    copy and redistribute the article in any medium or format
  • ADAPT:
    remix, transform, and build upon the article (eg.: to produce a new research work and, possibly, a new publication)
  • ALIKE:
    If you remix, transform, or build upon the article, you must distribute your contributions under the same license as the original.
  • NO ADDITIONAL RESTRICTIONS:
    You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

It does however mean that when you use it you must:

  • ATTRIBUTION: You must give appropriate credit to both the Author(s) and the journal, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.

You may not:

  • NONCOMMERCIAL: You may not use the article for commercial purposes.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.