The Effect of Rosella (Hibiscuss sabdariff a L.) Powder on CD4 Counts in Healthy Volunteers

  • Dini Mardhiyani UNIVERSITAS AHMAD DAHLAN
  • Endang Darmawan UNIVERSITAS AHMAD DAHLAN
  • Akrom Akrom UNIVERSITAS AHMAD DAHLAN

Abstract

Roselle flower (Hibiscus sabdariffa L.) is beneficial as an immunostimulant because of its antioxidant property. Antioxidants in roselle flower are hibiscus anthocyanin, quercetin, L-ascorbic acid and protocatechuic acid. Any potential plants for pytopharmacy should be proven beneficial and safe through preclinical and clinical trial. The purpose of this clinical trial was to determine the effect of ethanol extract of roselle (Hibiscus sabdariffa L.) to lymphocyte and CD4 cell count in healthy volunteer for 30 days.


This study used pre-post treatment design. A total of 21 healthy volunteer were chosen according to inclusion and exclusion criteria and filled informed consent. Healthy volunteer were given 500 mg roselle ethanol extract powder every day for 30 days. Lymphocyte and CD4 cell count was conducted using hematology analyzer and flowcytometer. Results were analyzed using paired t test, if the distribution is not normal than Wilcoxon test will be used on 95% confidence interval.


Results showed that there was no significant effect (p>0,05) of CD4 cell absolute count and there was a significant effect of CD4 percentage (p<0,05). Results on day 45 (14 days after the treatment was interrupted) showed no significant effect (p>0,05) of CD4 cell absolute count and CD4 cell percentage. The effect of CD4 cell percentage on day 31 was in normal range.


This study concluded that 500 mg roselle petal ethanol extract powder (Hibiscus sabdariffa L.) given everyday for 30 days showed some effect on d increasing CD4 cell percentage.

References

1. Akrom, Nurani LN, Hidayati T. Kajian aktivitas imunomodulator agen kemopreventif isolate aktif ekstrak N.sativa pada kanker payudara akibat paparan DMBA pada tikus putih, [Research report], LPP UAD, 2008. Yogyakarta.

2. Asano Y and Hodes RJ. T cell regulation of B cell activation. An antigen-mediated tripartite interaction of Ts cells, Th cells, and B cells is required for suppression. J. Immunol. 1984. 133: 2864-2867.

3. Chauhan PS, Satti NK, Suri KA, Amina M. Bani S. Stimulatory Effect of Cuminum cyminum and flavonoid glycoside on Cyclosporine-A and restraint stress induced immune-suppression in Swiss albino mice, Chemico-Biological- Interaction. 2010. 185(1):66-72.

4. Dharmana E, Susilaningsih N and Widjayahadi N. Pengaruh Pemberian Tolak Angin Cair terhadap Jumlah Sel T, Kadar IFN-γ, dan IL-4. 2007. Fakultas Kedokteran Universitas Diponegoro, Semarang. Diambi dari URL:http://eprints.undip.ac.id/23621/1/Narottama.pdf. diakses tanggal 23 Juli 2017

5. Dwijayanti DR, Muhaimin Rifa'I. Gynura procumbens Ethanolic Extract Promotes Lymphocyte Activation and Regulatory T Cell Generation In Vitro, The Journal Of Tropical Life Scienc. 2015. VOL. 5, NO. 1, pp. 14-19.

6. Food and Drug Administration (FDA)., 2010. Guidance for Industry M3(R2) Nonclinical Safety Studies for the Conduct of Human Clinical Trials and Marketing Authorization for Pharmaceuticals: U.S. Department of Health and Human Services. Diambil dari URL:https://www.fda.gov/downloads/drugs/guidances/ucm073246.pdf. diakses tanggal 19 Januari 2017.

7. Galeotti M. Some aspects of the application of immunostimulants and a critical review of methods for their evaluation, J. Appl. Ichthyol. 1998. 14: 189-199

8. Hirunpanich V, Utaipat A, Morales NP, Bunyapraphatsara N, Sato H, Herunsalee A, et al. Antioxidant effects of aqueous extracts from dried calyx of Hibiscus sabdariffa Linn. (Roselle) in vitro using rat low-density lipoprotein (LDL). Biological & Pharmaceutical Bulletin. 2005. 28(3): 481–484.

9. Juniarka IGA, Lukitaningsih ENS. Analisis Aktivitas Antioksidan dan Kandungan Antosianin Total Ekstrak Dan Liposom Kelopak Bunga Rosella. 2011. Majalah Obat Tradisional, 16(3).

10. Kurniasih. Perilaku dinamik model matematika atas peran IL-2 (Interleukin-2), sel helper CD4+ dan sel T regulatori. Skripsi. Fakultas Sains dan Teknologi Universitas Islam Negeri Maulana Malik Ibrahim, Malang. 2012. Hal 22-24

11. Lehner MD. Immunomodulation by Endotoxin Tolerance in Murine Models of Inflammation and Bacterial Infection, [Disertasi]. University of Konstanz. 2001. hal 3-53

12. Lu FC and Kacew S. Lu’s Basic Toxicology : Fundamental, Target Organs, 5th Ed. Informa Healthcare USA Inc., New York. 2009: 48-49,

13. Lu FC Kacew. S. Lu’s Basic Toxicology : Fundamental, Target Organs, 5th Ed. Informa Healthcare USA Inc., New York. 2009: 48-49,

14. Lu L and Rudensky A. Molecular orchestration of differentiation and function of regulatory T cells, Genes Dev. 2009. 23:1270-1282.

15. Middleton E. Kandaswami C. Theoharides TC. The effects of plant Flavonoids on mammalian cells: implications for inflammation, heart disease, and cancer, Pharmacology, The American Society for Pharmacology and Experimental Therapeutics . 2000. 52: 673-751.

16. Nurkhasanah. The Effect Of Rosella (Hibiscus Sabdariffa L) Treatment On Il-10 And Il-14 Secretion On Dimethylbenz (A) Anthracene (Dmba) Induced Rat, International Journal of Pharmacy and Pharmaceutical Sciences Vol 7, Issue 4, 2015 P.402-404

17. Passerini L, Allan SE, Battaglia M, Nunzio SD, Alstad AN. Levings, MK., Roncarolo MG, Bacchetta R. STAT5-Signaling cytokines regulate the expression of FOXP3 in CD41CD251 regulatory T cells and CD41CD252 effector T cells, International Immunology. 2008. 20(3): 421-431.

18. Prasetyo AA and Khilyat UNZ. Establishing mean CD4+ t cell values among healthy javanese adults in indonesia, Southeast Asian J Trop Med Public Health. 2008. 46(4):662-8.

19. Roitt IM. Roitt’s Essential Immunology, Ninth Edition. Blackwell Scientific Publications, London. 1997. 168-178

20. Rosidah. Analisis numerik untuk immunotherapy pada infeksi HIV-1 [Tesis]. Bogor: Sekolah Pascasarjana Institut Pertanian Bogor, Bogor. 2010. hal 6-18

21. Sakai M. Current research status of fish immunostimulants. Aquaculture. 1999.172: 63-92

22. Shen CY, Wen-Li Zhang, Jian-Guo, Jiang. Immune-enhancing activity of polysaccharides from Hibiscus sabdariffa Linn. via MAPK and NF-kB signaling pathways in RAW264.7 cells, Journal of Functional Foods. 2017. 34: 118–129

23. Suwandi T. Pengembangan potensi antibakteri kelopak bunga Hibiscus Sabdariffa L. (rosela) terhadap streptococcus sanguinis penginduksi gingivitis menuju obat herbal terstandar, [Disertasi], Universitas Indonesia, Jakarta. 2012. Hal 199

24. Widyanto, Poppy and A. Nelistya. Rosella Aneka Olahan, Khasiat dan Ramuan. Depok : Penebar Swadaya. 2009. Hal 1-14

25. Zom E. Nelson EA, Mohseni M, Porcheray F, Kim H. IL-2 regulates FOXP3 expresions in human CD4CD25 regularity T cells through a STAT-dependent mechanism and induces the expansion of these cells in vivo, Blood. 2006. 108:1571-1578

26. Bluestone JA, Abbas AK. Natural versus adaptive regulatory T cells. Nat Rev Immunol 2003;3:253–7
Published
2018-10-30
How to Cite
MARDHIYANI, Dini; DARMAWAN, Endang; AKROM, Akrom. The Effect of Rosella (Hibiscuss sabdariff a L.) Powder on CD4 Counts in Healthy Volunteers. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 16, n. 2, p. 194-199, oct. 2018. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/530>. Date accessed: 31 may 2023. doi: https://doi.org/10.35814/jifi.v16i2.530.
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Articles