Identifikasi Lektin Umbi dari Typhonium flagelliforme (Lodd.) Blume

  • MUHAMMAD ALFARABI INSTITUT PERTANIAN BOGOR
  • SISWA SETYAHADI Badan Pengkajian dan Penerapan Teknologi
  • MARIA BINTANG INSTITUT PERTANIAN BOGOR
  • MIFTAHUDIN MIFTAHUDIN INSTITUT PERTANIAN BOGOR
  • CHAIDIR CHAIDIR UNIVERSITAS PANCASILA

Abstrak

Typhonium flagelliforme (keladi tikus) is a herbaceous plant that is widely distributed in Indonesia. The plant produces tuber containing lectin protein that is considered to have anticancer activity. However, there is no sufficient information about tuber lectin of T. flagelliforme originated from Indonesia. The objective of the research was to identify and characterize tuber lectin from seven accession of T. flagelliforme from Indonesia. Tubers were harvested from 6 month-old plants of T. flagelliforme. Protein content, toxicity, and hemagglutination activities of the tuber crude protein extracts were determined. The accession with highest hemagglutination activity of tuber protein was used for tuber lectin purification using DEAE-Sepharose and CM monolitic column. The results showed that tuber protein from all accessions have hemagglutination and toxicity activities. Solok accession has the highest total soluble tuber protein and hemagglutination activity compared than those of the other accessions. The accession contained 12,67 kDa lectin protein. The Solok tuber lectin was thermally stable at 20 to 40 °C, and relatively stable at pH 5.0 to 7.2. Those protein characteristics of tuber lectin will be useful to develop lectin based anticancer medicine.

Diterbitkan
2017-09-04
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ALFARABI, MUHAMMAD et al. Identifikasi Lektin Umbi dari Typhonium flagelliforme (Lodd.) Blume. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 14, n. 1, p. 73-79, sep. 2017. ISSN 2614-6495. Tersedia pada: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/54>. Tanggal Akses: 10 aug. 2025
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Vol 14 No 1 (2016): JIFI
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