Effect of Spirulina Platensis on Level Analysis, Histopathology, Insulin and Glut-4 Expression in Wistar Rats Induced by Streptozotosin

  • Kintoko Kintoko Universitas Ahmad Dahlan
  • Rifqi Ferry Balfas Universitas Muhadi Setaibudi
  • Nura Ustrina Universitas Ahmad Dahlan
  • Sitarina Widyarini Universitas Gadjah Mada
  • Lintang Cahya Saputri Universitas Ahmad Dahlan
  • Anandita Nurwijayanti Universitas Ahmad Dahlan
  • Fajar Slamet Riana Universitas Ahmad Dahlan
  • Neni Tri Anggraini Universitas Ahmad Dahlan

Abstract

Based on Basic Health Research, in Yogyakarta the incidence of diabetes mellitus based on doctor's diagnosis is 2.6% and symptoms will increase with age, but will decrease from age> 65 years. In the treatment of diabetes mellitus there are several treatments with synthetic drugs and natural ingredients for natural ingredients such as Spirulina platensis. Spirulina platensis is one of the microalgae that contains the natural spectrum of carotene and xantophyll pigment mixtures, and with fikocyanin has antioxidant activity, and Spirulina platensis can show a decrease in blood sugar.


The type of research conducted is an experimental study. Spirulina platensis is made with several doses of 36 mg, 72 mg, and 144 mg. Rats were divided into 6 groups, 5 groups of streptozotosin induced at a dose of 45 mg / kgBW until the mice had DM were characterized by high KGD yield, then given spirulina suspension, measured in sugar levels on days 0-28. After that the mice in the blood and then analyzed the effect of Spirulina platensis on creatinine and urea in blood. The results were analyzed using SPSS ANOVA and Post Hoc Test methods with 95% confidence level.


The results of the analysis of levels in various groups showed that in the induction of streptozotocin treated with spirulina plantesis various doses there was improvement in each analysis of the levels obtained, and also at the dose STZ+SP 36 in each analysis can improve the level of analysis. While on histopathology result and insulin expression in pancreas and histology and expression of glucose transporter 4 and histopathology on kidney and liver showed good change at various treatment dose group especially in STZ+SP 36 group. So at STZ+SP 36 dose showing the change both in this study.

References

1. Amanatin, D, Riesya. Pengaruh Kombinasi Konsentrasi Media Ekstrak Tauge (MET) dengan Pupuk Urea terhadap Kadar Protein Spirulina sp., Sains dan Seni Pomits. 2013. 2(2) : 2337-3520

2. Abed, S.A., El-Shazely, M.O., Ahmed, K.A., Abedel-mawla, E.M., and Ibrahim, A.K. Therapeutic Effects of Spirulina platensis on streptozotocin-Induced Diabetic Rrats, Egypt. J. Comp. Path & Clinic Path. 2015. 28 (1):18-31

3. Barus, D.A. 2013. Kandungan Fikosianin, Protein, dan Antioksidan Spirulina platensis Yang Dibutuhkan Dalam Media dan Umur Kultivasi Berbeda. pp.1–54.

4. Best, B., 2006, General Antioxidant Action,www.benbest.com/nutrceut/Antioxidant.html,Tanggal akses 14 Desember 2017.

5. Christwardana, M., Nur, M.M.A., Hadiyanto. 2013. Spirulina platensis: Potensinya Sebagai Bahan Pangan Fungsional, Jurnal Aplikasi Teknologi Pangan. 2(1).

6. Deng R, Chow TJ. Review: Hypolipidemic, Antioxidant, and Antiinflammatory Activities of Microalgae Spirulina. Cardiovaskular Therapeutics. 2010; 28:e33-e45

7. Desi Wulandari, 2012. hubungan dislipidemia dengan kadar ureum dan kreatinin darah pada penderita nefropati diabetic

8. Dwi A., R., Aulanni’am, Mahdi Chanif, 2014. Pengaruh Pemberian Herbal Spray Berbasis Bioaktif dari Spirulina (Spirulina sp.) terhadap Ekspresi iNOS pada Sel β-Pankreas dan Aktivitas Protease pada Luka Tikus DM
Tipe 1. Kimia Student Journal, Vol. 1 No. 1, pp. 64-70

9. Farouk K. El-Baz1, Hanan F. Aly , El-Sayed, and Amal A. Mohamed, 2013. Role of Spirulina platensis in the control of glycemia in DM2 rats.

10. Firdiyana, F. Agustini, T.W. Ma’ruf, W.F. 2015. Extraction of bioactive compounds as natural antioxidants from fresh spirulina plantesis using different solvents. JPHPI. Vol 18 (1).

11. Ghasemi, A., Khalifi, S., and Jedi, S. Streptozotocin-nicotinamide-induced Rat STZ of Type 2 Diabetes, Acta Physiologica Hungaria. 2014. 101(4):408-420

12. Guyton A. C, Hall J.E., 2014. Buku Ajar Fisiologi Kedokteran (Terjemahan). 12th ed. Setiawan I, editor. Jakarta: Saunder Elsevier

13. Haq, M., 2016. Pengaruh Variasi Konsentrasi Pati Ubi Talas (Colocasia esculenta L.) Sebagai Suspending Agent Terhadap Sifat Fisik Suspensi Asam Mefenamat.

14. Khotib, J., Pengaruh Vanadil Sulfat Terhadap Aktivitas Glucose Transporter 4 Jaringan Otot dan Adiposa Mencit (Mus Musculus) yang Menderita Diabetes Mellitus. 2010. 8(1), pp.36–43.

15. M.P. Okky dan B.W. Simon, Uji Efek Ekstrak Air Daun Pandan Wangi terhadap Penurunan Kadar Glukosa Darah dan Histopatologi Tikus Diabetes Mellitus. Jurnal Pangan dan Agroindustri. 2014. 2(2) p.16-27.

16. Nayak, B.S., Pereira, L.P. & Maharaj, D. Wound healing activity of Carica papaya L. in experimentally induced diabetic rats. Indian journal of experimental biology. 2007. 45(8), p.739.

17. N.Senthil, P.M.Balu, K. Murugesan, 2013. AntiHyperglycemic effect of Spirulina, Insulin and Morinda citrifolia against Streptozotocin induced diabetic rats.

18. Ocktarini, R. 2010. Pengaruh ekstrak herba anting-anting (Acalupha Australis L.) terhadap kadar glukosa darah mencit babl/c induksi streptozotocin. Skripsi. Universitas Sebelas Maret Surakarta.

19. Satyantini,W.H. 2013. Teknologi Produksi Fikosianin Spirulina platensis dan Pemanfaatannya Sebagai Imunostimulan pada Ikan Kerapu Bebek (Cromileptes altivelis), Tesis, Sekolah Pasca Sarjana Institut Pertanian
Bogor, Bogor.

20. Surbakti, T.R. Aktivitas Antihiperglikemik dan Antioksidan dari Spirulina platensis pada Umur Panen yang Berbeda, Skripsi, Fakultas Perikanan dan Ilmu Kelautan Institut Pertanian Bogor, Bogor. 2013.

21. Ummah, M., 2015. Efek Ekstrak Kayu Manis ( Cinnamomum cassia ) terhadap kadar glukosa darah, berat
badan, dan Low Density Lipoprotein (LDL) pada tikus yang diinduksi streptozotosin

22. Wulandari, D.A. 2016. Ekstraksi dan Aktivitas Inhibisi Spirulina platensis Terhadap Plasmodium falciparum 3D7 Penyebab Malaria Secara in-vitro, Tesis, Sekolah Pascasarjana Institut Pertanian Bogor.
Published
2018-10-30
How to Cite
KINTOKO, Kintoko et al. Effect of Spirulina Platensis on Level Analysis, Histopathology, Insulin and Glut-4 Expression in Wistar Rats Induced by Streptozotosin. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 16, n. 2, p. 238-247, oct. 2018. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/541>. Date accessed: 19 may 2024. doi: https://doi.org/10.35814/jifi.v16i2.541.
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Articles