Optimization of W/O/W Multiple Nanoemulsion of Madeira vein Leaves Extract and Glycolic Acid-Chitosan Conjugate Using Box-Behnken Design

  • Malinda Prihantini Institut Teknologi Bandung
  • Irda Fidrianny Sekolah Farmasi, Institut Teknologi Bandung

Abstract

Skin premature aging which is characterized by fine wrinkles can be overcome by stimulating collagen production and inhibiting its degradation, and also reducing corneocyte buildup. Madeira vein has been shown to induce collagen production and inhibit its degradation, while glycolic acid increase desquamation. The aim of this research was to optimize W/O/W multiple nanoemulsion formula of Madeira vein-leaves extract in W1 phase and glycolic acid in W2 phase. Glycolic acid was added after conjugated with chitosan to reduce its stinging effect on skin. The W/O/W multiple nanoemulsion was prepared through two emulsification steps: W1/O primary emulsion using high speed homogenizer, and the secondary emulsion using magnetic stirrer. W1/O was optimized including the selection of co surfactant and extract concentration, followed by secondary emulsion optimization using response surface methodology consisted of 2-level factorial and Box-Behnken design. The W1/O consisted of extract, polysorbate 80 as surfactant, PEG 400 as co surfactant and isopropyl myristate as oil phase. The 2-level factorial gave the three significant factors: W1/O sonication time, W1/O concentration, and secondary emulsification stirring time. Box-Behnken optimization for globule size below 400 nm was obtained by 6-10 minutes W1/O sonication time, 12-16% W1/O concentration, and 32-42 minutes stirring time of secondary emulsification.

References

1. Ganceviciene, Ruta, et al. Skin anti-aging strategies. Dermato-endocrinology. 2012. 4(3): 308-319.

2. Baumann L. Cosmetic dermatology principle and practice 2nd ed. New York: The McGraw-Hill Companies. 2009. p. 3-8, 34-38.

3. Draelos ZD. Cosmetic dermatology products and procedure 2nd ed. West Sussex: John Wiley & Sons. 2016. p. 5.

4. Raust AA. Pengembangan formula matriks berbasis mikropartikel kitosan sebagai sistem penghantaran EEDB untuk kultivasi sel fibroblast [tugas akhir]. Bandung: Program Sarjana Institut Teknologi Bandung; 2012.

5. Amanda P. Isolasi suatu senyawa antioksidan dari ekstrak etil asetat daun binahong (Anredera Cardifolia (Ten.) Steenis [tugas akhir]. Bandung: Program Sarjana, Institut Teknologi Bandung; 2012.

6. Sharad, J., (2013): Glycolic acid peel therapy – a current review, Dove Press Journal: Clinical, Cosmetic and Investigational Dermatology, 6, 281-288.

7. Rosen M. Delivery system handbook for personal care and cosmetic products: technology, applications and formulations. New York: William Andrew. 2005. p. 881-908.

8. Kovács, A., Erős, I., & Csóka, I., (2016): Optimization and development of stable w/o/w cosmetic multiple emulsions by means of the Quality by Design approach, International Journal of Cosmetic Science, 38 (2), 128-138.

9. Verma, D. D., Verma, S., Blume, G., & Fahr, A., (2003): Particle size of liposomes influences dermal delivery of substances into skin. International Journal of Pharmaceutics, 258 (1-2), 141-151.

10. Bezerra, M. A., Santelli, R. E., Oliviera, E. P, Villar, L. S., dan Escaleira, L.A., (2008): Response surface methodology (RSM) as a tool for optimization in analytical chemistry, Talanta, 76, 965 – 977.

11. Kementerian Kesehatan RI. Direktorat Jenderal Bina Kefarmasian dan Alat Kesehatan, (2011): Suplemen II Farmakope Herbal Indonesia edisi II, Jakarta: Kementerian Kesehatan RI.

12. Dwiani, R. R. S., (2012): Formulasi emulsi ganda A/M/A α – Arbutin dan uji aktivitas inhibisi sediaan terhadap enzim tirosinase, Tugas akhir Program Sarjana, Institut Teknologi Bandung.

13. Pamudji, J. S., Mauludin, R., Indriani, N., (20I6): Development of nanostructured lipid carrier formulation containing of retinyl palmitate. Int J Pharm Pharm Sci, 8 (2), 256-260.

14. Tofani, R. P., Sumirtapura, Y. C., & Darijanto, S. T., (2016): Formulation, characterisation, and in vitro skin diffusion of nanostructured lipid carriers for deoxyarbutin compared to a nanoemulsion and conventional cream. Scientia Pharmaceutica, 84 (4), 634-645.

15. Badan Pengawas Obat dan Makanan Republik Indonesia (BPOM RI), (2014): Peraturan Kepala Badan Pengawas Obat dan Makanan no 7 tentang pedoman uji toksisitas nonklinik secara in vivo, BPOM RI, Jakarta.

16. OECD, (2015): Acute dermal irritation/corrosion. in OECD guidelines for the testing of chemicals section 4 no. 404, OECD, Paris.

17. Raust, A., A., (2017): Pengembangan formula matriks berbasis mikropartikel kitosan sebagai sistem penghantaran ekstrak etanol daun binahong untuk kultivasi sel fibroblas, Tugas akhir Program Sarjana, Institut Teknologi Bandung.

18. Watson, David G., (2005): Pharmaceutical Analysis 2nd ed., United Kingdom, Oxford, 135, 195.

19. Mahmood, T., Akhtar, N., & Manickam, S., (2014): Interfacial film stabilized W/O/W nano multiple emulsions loaded with green tea and lotus extracts: systematic characterization of physicochemical properties and shelf-storage stabilit, Journal of nanobiotechnology, 12 (1), 20.

20. Wang, Y., (2014): Preparation of Nano- and Microemulsion using Phase Inversion and Emulsion Titration Methods, Thesis Master of Food and Technology, Massey University, 6 – 36.

21. Eichner, A., Stahlberg, S., Sonnenberger, S., Lange, S., Dobner, B., Ostermann, A., & Neubert, R. H., (2017): Influence of the penetration enhancer isopropyl myristate on stratum corneum lipid model membranes revealed by neutron diffraction and 2 H NMR experiments. Biochimica et Biophysica Acta (BBA)-Biomembranes, 1859 (5), 745-755.

22. Delmas, T., Piraux, H., Couffin, A. C., Texier, I., Vinet, F., Poulin, P., & Bibette, J., (2011): How to prepare and stabilize very small nanoemulsions, Langmuir, 27(5), 1683-1692.

23. Schmidts, T., Dobler, D., Nissing, C., & Runkel, F., (2009): Influence of hydrophilic surfactants on the properties of multiple W/O/W emulsions, Journal of Colloid and Interface Science, 338 (1), 184-192.

24. Benichou, A., Aserin, A., & Garti, N., (2004): Double emulsions stabilized with hybrids of natural polymers for entrapment and slow release of active matters, Advances in Colloid and Interface Science, 108, 29-41.

25. Schuch, A., Deiters, P., Henne, J., Köhler, K., & Schuchmann, H. P., (2013): Production of W/O/W (water-in-oil-in-water) multiple emulsions: droplet breakup and release of water, Journal of colloid and interface science, 402, 157-164.

26. Rezaee, M., Basri, M., Rahman, R. N. Z. R. A., Salleh, A. B., Chaibakhsh, N., & Karjiban, R. A., (2014): Formulation development and optimization of palm kernel oil esters-based nanoemulsions containing sodium diclofenac. International Journal of Nanomedicine, 9, 539.

27. Ngan, C. L., Basri, M., Lye, F. F., Masoumi, H. R. F., Tripathy, M., Karjiban, R. A., & Abdul-Malek, E., (2014):
Comparison of process parameter optimization using different designs in nanoemulsion-based formulation for transdermal delivery of fullerene, International Journal of Nanomedicine, 9, 4375.

28. Saddler, David, Shea, Julia, Ward, Derek., (2011): 12 B Stationary Points and Turning Points, Cambridge 2 Unit Mathematics Year 11, Cambridge University Press, 318.

29. Grumezeschu A. M., (2016): Emulsions nanotechnology in the agri-food industry vol. 3, Academic Press Elsevier, London, 18-19.

30. Ribeiro, R. C. D. A., Barreto, S. M. A. G., Ostrosky, E. A., Rocha-Filho, P. A. D., Veríssimo, L. M., & Ferrari, M., (2015): Production and characterization of cosmetic nanoemulsions containing Opuntia ficus-indica (L.) mill extract as moisturizing agent, Molecules, 20 (2), 2492-2509.

31. Iqbal, M., Zafar, N., Fessi, H., & Elaissari, A., (2015): Double emulsion solvent evaporation techniques used for
drug encapsulation, International journal of pharmaceutics, 496 (2), 173-190.

32. Ferreira, L. A. M., Seiller, M., Grossiord, J. L., Marty, J. P., & Wepierre, J., (1995): Vehicle influence on in vitro release of glucose: w/o, w/o/w and o/w systems compared, Journal of controlled release, 33 (3), 349-356.
Published
2019-10-29
How to Cite
PRIHANTINI, Malinda; FIDRIANNY, Irda. Optimization of W/O/W Multiple Nanoemulsion of Madeira vein Leaves Extract and Glycolic Acid-Chitosan Conjugate Using Box-Behnken Design. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 17, n. 2, p. 150-159, oct. 2019. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/556>. Date accessed: 18 nov. 2019. doi: https://doi.org/10.35814/jifi.v17i2.556.
Section
Articles