Immunomodulatory Activity of Several Plants from Piperaceae Family in Balb/C Mice with Carbon Clerance Method

  • Mohammad Roseno Poltekkes Kemenkes Bandung
  • Yayat Sudaryat Jurusan Farmasi, Politeknik Kesehatan Kemenkes Bandung
  • Widyastiwi Widyastiwi Jurusan Farmasi, Politeknik Kesehatan Kemenkes Bandung
DOI: https://doi.org/10.35814/jifi.v17i2.558

Abstract

Plants of Piperaceae family are widespread in the tropics region and have been widely used as medicinal plants, these includes kemukus (Piper cubeba), kiseureuh (Piper aduncum), and Java chili (Piper retrofractum). The purpose of this study was to evaluate the immunomodulatory activity of ethanol extracts of several plants from the Piperaceae family in Balb/C strain mice with carbon clearance method. The study was conducted by dividing the experimental animals into 12 groups : normal control group, immunosuppressant comparison, immunostimulant comparison, and low, moderate, and high dose of kemukus, kiseureuh, and Java chili, resepctively. Immunomodulatory activity was measured by phagocytosis index, peripheral blood leukocyte levels, and splenic leukocyte levels. The results showed that ethanol kemukus extract (Piper cubeba) acts as an immunosuppressant, increasing the dose increased the immunosuppressant effect. Low doses of ethanol extract of kiseureh (Piper aduncum) also acts as  immunosuppressant, whereas at medium and high doses has a tendency to be immunostimulant. Ethanol extract of Java chili (Piper retrofractum) acts as an immunostimulant, with the highest activity achieved at moderate doses. Ethanol extract of kemukus (Piper cubeba), kiseureh (Piper aduncum), and Java chili (Piper retrofractum) affect the number of peripheral blood granulocyte components, neutrophils levels affects the increase in phagocytic effects. Ethanol extract of kemukus (Piper cubeba), kiseureh (Piper aduncum), and Java chili (Piper retrofractum) did not affect the number of splenic blood granulocyte components.


 

References

1. Wattimena RJ. Farmakodinami dan Terapi Antibiotik. Yogyakarta: Gadjah Mada University Press; 1991. p. 1 – 17.

2. Katzung B. Farmakologi Dasar dan Klinik. VI. Jakarta: ECG; 2011.

3. Krisna S.B. Imunologi: Diagnosis dan Prosedur Laboratorium. 5th ed. Jakarta: Fakultas Kedokteran Universitas Indonesia; 2010.

4. Global Asthma Network. The Global Asthma Report 2014. Vol. 5. 2014. p. 14-16.

5. Lerner A, Jeremias P, Matthias T. The World Incidence and Prevalence of Autoimmune Diseases is Increasing. Int J Celiac Dis. 2016.3(4):151–155.

6. Hall PD, Weimert NA. Function and Evaluation of the Immune System. In: DiPiro JT, Talbert RL, Yee GC, Matzke GR, Wells BG, Posey LM, editors. Pharmacotherapy A Pathophysiologic Approach. 7th ed. New York: McGraw Hill; 2008. p. 1417–1430.

7. Mustshler E. Dinamika Obat Buku Ajar Farmakologi dan Toksikologi. Bandung: Penerbit ITB; 1991. p. 697-699.

8. Skidgel RA, Erdos EG. Autocoids : Drug Therapy of Inflammation. In: Brunton LL, Lazo JS, Parker KL, editors. Goodman & Gilman’s The Pharmacological Basis of Theurapeutics. 11th ed. New York: McGraw Hill; 2006.

9. Ghosh R, Darin K, Nath P, Deb P. An Overview of Various Piper Species for Their Biological Activities. Int J Pharma Res Rev. 2014.3(1):67–75.

10. Nahak G, Sahu RK. Phytochemical Evaluation and Antioxidant activity of Piper cubeba and Piper nigrum. J Appl Pharm Sci. 2011.01(8):153–7.

11. Alsaid M, Mothana R, Raish M, Al-Sohaibani M, Al-Yahya M, Ahmad A, et al. Evaluation of the effectiveness of Piper cubeba extract in the amelioration of CCl4-induced liver injuries and oxidative damage in the rodent model. Biomed Res Int. 2015;2015.

12. Pachpute AP, Deshmukh TA, Jagdishprasad S, Tibrewal J. Antioxidant and Hepatoprotective Activity of an Ethanol Extract of Piper cubeba Fruits. Int J Res Dev Pharm Life Sci. 2013.2(2):321–329.

13. Graidist P, Martla M, Sukpondma Y. Cytotoxic Activity of Piper cubeba Extract in Breast Cancer Cell Lines. Nutrients. 2015.7:2707–2718.

14. Ahmad QZ, Jahan N, Ahmad G, Tajuddin. Nephroprotective Effect of Kabab Chini (Piper cubeba) in Gentamycin- Induced Nephrotoxicity. Saudi J Kidney Dis Transplant. 2017.23(4):1–7.

15. Esperandim VR, Ferreira DS, Rezende KCS, Magalhaes LG, Souza JM, Pauletti PM, et al. In Vitro Antiparasitic Activity and Chemical Composition of the Essential Oil Obtained from the Fruits of Piper cubeba. Planta Med. 2013.79:1653–1655.

16. Syahroni YY, Prijono D. Aktivitas Insektisida Campuran Ekstrak Buah Piper aduncum (Piperaceae) dan Sapindus rarak (Sapindaceae) terhadap Larva Crocidolomia pavonana. J Entomol Indones. 2013.10(1):39–50.

17. Brazao MA, Brazao F, Maia JG, Monteiro M. Antibacterial activity of the Piper aduncum oil and dillapiole, its main constituent, against multidrug-resistant strains. Boletín Latinoam y del Caribe Plantas Med y Aromáticas. 2014.13(July):517–526.

18. Chansang U, Zahiri NS, Bansiddhi J, Boonruad T, Thongsrirak P, Mingmuang J, et al. Mosquito larvicidal activity of aqueous extracts of long pepper (Piper retrofractum Vahl) from Thailand. J Vector Ecol. 2005.30(2):195–199.

19. Rahmawati N, Bachri MS. The aphrodisiac effect and toxicity of combination Piper retrofractum L, Centella asiatica, and Curcuma domestica infusion. Aphrodisiac Eff Infus. 2012.3(1):19–22.

20. Arroyo-acevedo J, Chávez-asmat R., Anampa-Guzmán A, Donaires R, Ráez-Gonzáles J. Protective Effect of Piper aduncum Capsule on DMBA- induced Breast Cancer in Rats. Breast Cancer Basic Clin Res. 2015.9:41–48.

21. Tihnulat ANU. Efek Bawang Putih (Allium sativum) dan Cabe Jawa (Piper retrofractum Vahl.) Terhadap Jumlah Neutrofil Pada Tikus yang Diberi Suplemen Kuning Telur [skripsi]. Universitas Diponegoro; 2009.

22. Rosales C, Demaurex N, Lowell CA, Uribe-Querol E. Neutrophils: Their Role in Innate and Adaptive Immunity. J Immunol Res. 2016.
Published
2019-10-29
How to Cite
ROSENO, Mohammad; SUDARYAT, Yayat; WIDYASTIWI, Widyastiwi. Immunomodulatory Activity of Several Plants from Piperaceae Family in Balb/C Mice with Carbon Clerance Method. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 17, n. 2, p. 255-261, oct. 2019. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/558>. Date accessed: 26 dec. 2024. doi: https://doi.org/10.35814/jifi.v17i2.558.
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