Isolation of Black Garlic Polyphenol Compounds and Toxicity Tests on Shrimp Larvae (Artemia salina Leach)

  • Santi Nur Handayani Universitas Jenderal Soedirman
  • Lidya Cahyo Bawono Universitas Jenderal Soedirman
  • Diana Pramesti Ayu Universitas Jenderal Soedirman
  • Hesti Nurrizqi Pratiwi Universitas Jenderal Soedirman

Abstract

Cancer is one of diseases caused by cell division that is uncontrollable. Cervical cancer is a cancer that suffered by many women. Alternative medicine of cancer disease is use black garlic. Black garlic is the result of processing the garlic with high temperature and humidity without addition of other substances. In that process occur Maillard reaction that cause changes in the color, smell, and taste of the original on the garlic. Such reaction also lead to an increase some bioactive compounds such as S-allyl cysteine ​​(SAC), amino acids, flavonoids, and polyphenols. To determine the toxic properties of black garlic extract, it is necessary to do isolation of polyphenolic compounds and toxicity test against Artemia salina Leach. When calculated by SPSS application, the obtained LC50values of black garlic extract on the aging prosess time variations of 30 days and 40 days showed concentration of 682.433 and 572.403 ppm.

References

1. Carballo, J.L., Zaira L. H., Pilar P., and Maria D.G. A Comparison Between Two Brine Shrimp Assays to Detect in Vitro Cytotoxicity in Marine Natural Product. BMC Biotecnol 10. 2002. 1186/1472-6750; 2:17.
2. Choi, S., H.S. Cha and Y.S. Lee. Physicochemical and Antioxidant Properties of Black garlic. Molecules. 2014. 19: 16811-16823
3. Choi, Duk Ju., et al. Physicochemical Characteristics Of Black garlic (Allinum sativum). J Korean Soc.Food Nutr. 2008. 37(4) : 465-471
4. Danan W, Yonghui F, Jun L, Jianzhong Y, Meiru W, Jin-ichi S, Changlong L. Black garlic (Allinumsativum). J Medicinal and Aromantic Plant Science and Biotechnology. 2010. 4(1) : 37-40.
5. Hari S, Riza Alfian. Determination Of Total Phenolic Content Of Methanolic Extracts Red Rosell (Hibiscus sabdariffa Linn) Calyxs In Variation Of Growing Area By Spectofotometery. J Ilmiah Kefarmasian. 2012. 2(1) : 73-80.
6. Hoshino T, Kashimoto N, Kasuga S. Effects of garlic preparations on the gastrointestinal mucosa. J Nutr. 2001. 131(3): S1109-13.
7. Kimura, S., Y.C. Tung., M.H. Pan., N.W Su.,Y.J. Lai and K.C. Cheng. Black garlic: A critical review of its production, bioactivity, and application. Journal of food and drug analysis. 2017. 25(1) : 62-70.
8. Kim JH, Nam SH, Rico CW, Kang MY. A Comparative Study on the Antioxidative and Anti-allergic Activities of Fresh and Aged Black Garlic Extracts. Int J Food Sci Tech. 2012. 47(6):1176-82.
9. Lybarger J.A., Gallagher J.S., Pulver D.W., Litwin A, Brooks S, Bernstein I.L. Occupational Asthma Induced by Inhalation and Ingestion of Garlic. J Allergy Clin Immun. 1982. 69(5):448-54.
10. Xu, G., Ye X., Chen J., Liu D. Effect of Heat Treatment on The Phenolic Compounds and Antioxidant Capacity of Citrus Peel Extract. J. Agric. Food Chem. 2007. 55(2): 300-5
11. Villalón, A.L. G., S. Amor, L. Monge, N. Fernández, M. Prodanov, M. Muñoz, A.M. Inarejos-García, and M. Granado. In vitro studies of an aged black garlic extract enriched in S-allylcysteine and polyphenols with cardioprotective effects. Journal of functional foods. 2016. 27: 189–200
12. Lu, X., N. Li., X. Qiao., Z. Qiu, and P. Liu. Composition Analysis and Antioxidant Properties of Black Garlic Extract. Journal of food and drug analysis. 2016. 1-10
Published
2018-10-30
How to Cite
HANDAYANI, Santi Nur et al. Isolation of Black Garlic Polyphenol Compounds and Toxicity Tests on Shrimp Larvae (Artemia salina Leach). JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 16, n. 2, p. 145-149, oct. 2018. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/561>. Date accessed: 17 sep. 2019. doi: https://doi.org/10.35814/jifi.v16i2.561.
Section
Articles