Analgesic and Anti-Inflammatory Activities of Eurycoma longifolia root in Mice

  • Phebe Hendra Universitas Sanata Dharma Yogyakarta
  • Wina Susana Universitas Sanata Dharma Yogyakarta
  • Indriyani Permatasari Universitas Sanata Dharma Yogyakarta
  • Jessy Florensia Universitas Sanata Dharma Yogyakarta
  • Monita Natalia Siregar Universitas Sanata Dharma Yogyakarta


This study was conducted to evaluate the analgesic and anti-inflammatory acitvities of Eurycoma longifolia root in mice. The analgesic effect was assessed using chemical stimuli (wrihting test) to induce peripheral pain in mice. The anti-inflammatory activity was determined using carrageenan-induced paw edema in mice. This study exhibites that Eurycoma longifolia root possesses analgesic and anti-inflammatory activities in mice.


1. Tjay TH dan Rahardja K. Obat - Obat Penting Edisi ke-6. Jakarta: PT. Elex Media Komputindo. 2007. hal. 312-313, 330.
2. Kumar S, Bajwaa BS, Kuldeep S, and Kalia AN. Anti-Inflammatory Activity of Herbal Plants: A Review, International Journal Of Advances In Pharmacy. Biology And Chemistry. 2013. 2: 272-81.
3. Ashley NT, Weil ZM, and Nelson RJ. Inflammation: Mechanisms Costs and Natural Variation. The Annual Review of Ecology Evolution and Systematics, 2012. 43: 385-406.
4. Cottrell J and Connor JP. Effect of Non-Steroidal Anti-Inflammatory Drugs on Bone Healing. Pharmaceuticals. 2010. 3: 1668-93.
5. Sostres C, Gargallo CJ, and Lanas A. Nonsteroidal Anti-Inflammatory Drugs and Upper and Lower Gastrointestinal Mucosal Damage. Arthritis Research & Therapy. 2013. 15(3): 1-8.
6. Elfahmi, Woerdenbag HJ, and Kayser O. Jamu: Indonesian Traditional Herbal Medicine Towards Rational Phytopharmacological Use. J Herbal Med. 2014. 23: 1-24.
7. Varghese CP, Ambrose C, Jin SC, Lim YJ, and Keisaban T. Antioxidant and Antiinflammatory Activity of Eurycoma longifolia Jack A Traditional Medicinal Plant in Malaysia. International Journal of Pharmaceutical Sciences and Nanotechnology. 2012. 4(5): 1875-8.
8. Han YM, Jang M, Kim IS, Kim SH, Yoo HH. Simultaneous Quantitation of Six Major Quassinoids in Tongkat Ali Dietary Supplements by Liquid Chromatography with Tandem Mass Spectrometry. J Sep Sci. 2015. 38: 2260-6.
9. Rehman SU, Choe K, and Yoo H. Review on a Traditional Herbal Medicine, Eurycoma longifolia Jack (Tongkat Ali): Its Traditional Uses, Chemistry, Evidence-Based Pharmacology and Toxicology. Molecules. 2016. 21 (331): 1-31.
10. Tran TV, Malainer C, Schwaiger S, Atanasov AG, Heiss EH, Dirsch VM, and Stuppner H. NF-κB Inhibitors from Eurycoma longifolia Jack. J. Nat. Prod. 2014. 77: 483-8.
11. Hajjouli S, Chateauvieux S, Teiten MH, Orlikova B, Schumacher M, Dicato M, et al. Eurycomanone and Eurycomanol from Eurycoma Longifolia Jack as Regulators of Sinyal Pathways Involved in Proliferation, Cell Death and Inflammation. Molecules. 2014. 19(9): 14640-6.
12. Niederberger E and Geisslinger G. The IKK-NF-Kb Pathway: A Source for Novel Molecular Drug Targets in Pain Therapy. The FASEB Journal. 2008. 22: 3432-42.
13. Lawrence T. The Nuclear Factor NF-kappaB Pathway in Inflammation. Cold Spring Harb Perspect Biol. 2009. 1(6): 1-10.
14. Doughari JH. Phytochemicals: Extraction Methods, Basic Structures and Mode Of Action as Potential Chemotherapeutic Agents. Phytochemical – A global perspective of their role in nutrition and health. China. 2012. 4.
15. Badan Pengawas Obat dan Makanan RI. Acuan Sediaan Herbal. Jakarta: Direktorat Obat Asli Indonesia. 2010. hal. 8.
16. Chaulya NC, Haldar PK, Mukherjee A. Anti-inflammatory and Analgesic Activity of Methanol Extracts of Cyperus tegetum Roxb. Rhizome. Journal of PharmaSciTech 2012. 1(2): 27-9.
17. Mishra D, Ghosh G, Kumar PS, and Panda PK. An Experimental Study of Analgesic Activity of Selective COX-2 Inhibitor with Conventional NSAIDs. Asian Journal of Pharmaceutical and Clinical Research. 2011. 4(1): 78-81.
18. Rahayu L, Dewi RS, Ayu G. Uji efek anti-inflamasi dan analgesic infusa daun senggani (Melastoma malabathricum L.). Jurnal Ilmu Kefarmasian Indonesia 2016.14 (1): 93-8.
19. Nguemfo EL, Dimo T, Azebaze AGB, Asongalem EA, Alaoui K, Dongmo AB, Cherrah Y, Kamtchouing P. Anti-inflammatory and anti-nociceptive activities of the stem bark extract form Allanblackia monticola STANER L.C. (Guttiferae). J Ethnopharmacol 2007. 114: 417-24.
20. Huang SS, Huang GJ, Peng WH, Ho YL, Chang MJ, Hung HJ, et al. Analgesic and Antiinflammatory Activities of an Aqueous Extract of Hydrocotyle batrachium Hance in Mice. Mid Taiwan J Med. 2008. 13: 179-85.
21. Nunes DC, Rodrigues RS, Lucena MN, Cologna CT, Oliveira AC, Hamaguchi A, et al. Isolation and Functional Characterization of Proinflammatory Acidic Phospholipase A2 from Bothrops leucurus Snake Venom. Comparative Biochemistry and Physiology. 2011. 154(3): 226-33.
22. Tjandrawinata RR, Djunarko I, Fenty, and Hendra P. Anti-inflammation Effects of Bioactive Fraction DLBS0533 Containing Phaleria Macrocarpa and Nigella Sativa on Animal Model. Int J Pharm Pharm Sci. 2015. 7(1): 408-11.
23. Deraedt R, Jouquey S, Delevallee F, Flahaut M. Release of prostaglandins E dan F in an algogenic reaction and its inhibition. European Journal of Pharmacology 1980. 61 (11): 17-24.
24. Necas J, Bartosikova L. Carrageenan: A Review. Veterinarni Medicina 2013; 58: 187-205.
25. Zhang J M, An J. Cytokines, Inflammation and Pain. Int Anesthesiol Clin. 2007. 45 (2): 27-37.
How to Cite
HENDRA, Phebe et al. Analgesic and Anti-Inflammatory Activities of Eurycoma longifolia root in Mice. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 16, n. 2, p. 182-187, oct. 2018. ISSN 2614-6495. Available at: <>. Date accessed: 17 sep. 2019. doi: