Analisis Glikoprotein dalam Daging Mytilus viridis, Anadara granosa, dan Anadara maculosa

  • Zuhelmi Aziz Universitas Pancasila
  • Thamrin Wikanta Universitas Pancasila
  • Tirta Subagio Universitas Pancasila

Abstract

Mussels is known to have ability living in clean or polluted waters environment due to it has a specific immunity system. The immunity mechanism is carried out by a glycoprotein that play role as an immunomodulator with high activity. Extraction of glycoprotein from fresh green mussel (Mytilus viridis), blood mussel (Anadara granosa) and bulu mussel (Anadara maculosa) have been carried out, by boiling the mussels for 30 minutes and 60 minutes, precipitation of glycoprotein with ethanol, then purification of glycoprotein through the Sephadex G-100 column. The amino acids composition of the glycoprotein was analyzed by high performance liquid chromatography (HPLC) with normal phase pico tag column, using 1 ml/min isocratic elution with sodium acetate buffer-acetonitril mixture, and detection with UV detector. Result showed that the yield of glycoprotein from 60 minutes boiling was higher than from 30 minutes boiling. The glycoprotein content of green mussel, blood mussel and bulu mussel were 11,97%, 8,36%, and 18,93% from 60 minutes boiling, and 10,32%, 7,93%, and 9,07 from 30 minutes boiling, respectively. It can be concluded that the amino acids composition in the glycoprotein from both treatment were not proportional , but the 60 minutes boiling was given higher result.

References

1. Balai Penelitian Perikanan Laut. Prosedur analisis kimia ikan dan produk olahan hasil-hasil perikanan. Jakarta: Balai penelitian Perikanan Laut; 1998. hal.1-16.

2. Millipore corporation. Liquid chromatography: analysis of amino acid in feeds and foods using modification of the pico-tag method. Millipore Corporation; 1987. p.1-10.

3. Departemen Kesehatan Republik Indonesia. Farmakope. Edisi IV. Jakarta: Departemen Kesehatan Republik Indonesia; 1995. hal.1002-16.

4. Copeland RA. Method for protein analysis. London: Chapman and Hall; 1993. p.26-38. 5. Murray RK. Glikoprotein. dalam: Murray, R.K., D. Anner, P.A. Mayes dan V.M. Rodwell (Eds.). Biokimia Harper. Eds. 24. Jakarta: EGC; 2002. p.668-86.



6. Sasaki T, Uchida H, Uchida NA, Takasuka N, Tachibana Y, Nakamichi K. Antitumor and immunomodulatory effect of scallop. Nippon Suisan Gakkaishi. 1987. 53(2): 287-72.

7. Skoog, DA, Leary JJ. Principles of Intrumental Analysis. 4ed. New York: Saunders College Publishing; 1996. p.628-67.
How to Cite
AZIZ, Zuhelmi; WIKANTA, Thamrin; SUBAGIO, Tirta. Analisis Glikoprotein dalam Daging Mytilus viridis, Anadara granosa, dan Anadara maculosa. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 5, n. 1, p. 1-6, apr. 2007. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/581>. Date accessed: 18 may 2024.
Section
Articles