Uji Aktivitas Enzim Xilanase Ekstraselular dan Intraselular Bakteri Endofitik Tanaman Brucea javanica (L.) Merr.

  • Shirly Kumala Universitas Pancasila
  • Wibowo Mangunwardoyo Universitas Pancasila
  • Diah Dethriani Universitas Pancasila

Abstract

Assay for xylanase enzyme activity of endophytic bacteria from Brucea javanica (L.) Merr. has been carried out. Parts of the plants used in this study were stem, leave and fruit. Samples were collected from Bogor, Cianjur and Tawangmangu. To evaluate the enzyme activity the fungi were shakefermented for 14 days. The supernatant were used to examine the extracellular enzyme and biomass for intracellular enzyme, using DNS methode to test the activity of enzyme. The results showed that from ten isolates only four isolates produced xylanase enzyme. Isolate 2.3.1 and 2.3.6 produced extra and intracellular enzyme, while isolate 2.1.15 and 2.3.13 only produced extracellular xylanase enzyme.

References

1. Wijayakusuma H. Tanaman berkhasiat obat di Indonesia. Jilid I. Jakarta: Penerbit Pustaka Kartini;
1997.hal.36-7.

2. Budi I. Buah merah. Jakarta: Penebar Swadaya; 2004.hal.22-51.

3. Paimin, Fendy R. Bukti ilmiah buah merah. Trubus. April 2005;hal.13-4.

4. Sutrisno RB. Analisis jamu. Edisi I. Jakarta: Fakultas Farmasi Universitas Pancasila; 1986.
hal.20-1.

5. Stahl E. Analisis Obat secara kromatografi dan mikroskopik. diterjemahkan oleh Kokasih P, Iwang S, Sofia N. Bandung: Institut Teknologi Bandung; 1985.hal.8-12, 52-3.

6. Departemen Kesehatan Republik Indonesia. Materia medika Indonesia. Jilid VI. Jakarta: Direktorat Jenderal Pengawasan Obat dan Makanan; 1995.hal.321-6.

7. World Health Organization. Quality control methods for medicinal plant materials. Geneva:
World Health Organization; 1992.p.26-8.

8. Ciulei J. Methodology for analysis of vegetable drugs. Romania: Faculty of Pharmacy Bucarest Romania. p. 17-26.

9. Meyer BN. Brine shrimp: a convenient general bioassay for active plant constituens. Planta Medica. 1982;45:31-4.
Published
2006-09-27
How to Cite
KUMALA, Shirly; MANGUNWARDOYO, Wibowo; DETHRIANI, Diah. Uji Aktivitas Enzim Xilanase Ekstraselular dan Intraselular Bakteri Endofitik Tanaman Brucea javanica (L.) Merr.. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 4, n. 2, p. 51-54, sep. 2006. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/588>. Date accessed: 30 nov. 2022.
Section
Articles