Gambaran Histopatologi Pankreas Tikus Hiperglikemia setelah Mengkonsumsi K-Karagenan dan í-Karagenan

  • Lestahari Rahayu Universitas Pancasila
  • Rahma Damayanti Universitas Pancasila
  • Thamrin Wikanta Pusat Riset Pengolahan Produk dan Sosial Ekonomi Kelautan

Abstract

Research on the effect of K-carrageen and i-carrageen feeding on the representation of pancreatic B-cell histopathology of hyperglycemic rat has been carried out. Rats were previously damaged by aloxan monohydrat induction. There were 6 groups of rats, each group containing 5 animals. Group I normal control, group II negative control, group III 1st positive control, group IV 2nd positive control, group V K-carrageen treatment, group VI i-carrageen treatment. Group I, II, IV, V, and VI, were given orally, while group III was given subcutaneously. All the treatments were given during 15 days. All the rats were then killed and pancreatic hystopathology were carried out. The results revealed that aloxan monohydrat induction resulting the rat pancreatic weight reduction in all treatment groups. The ratio of rat body weight to the pancreatic weight was not significant difference in all treatment groups. Insulin injection was unable to increase the size of Langerhans islet and unable to support the recovery process of pancreatic damaged due to aloxan induction. On the other hand, chlorpropamide, K-carrageenan, and i-carrageenan were able to increase the size of Langerhans islet because they can support the recovery process of pancreatic organ damaged due to aloxsan induction. Insulin injection was unable to increase the ratio of Langerhans islet diameter to the amount of B-cells. While, chlorpropamide, K-carrageenan, and i-carrageenan were able to increase the ratio of Langerhans islet diameter to the amount of ß-cells. The macroscopic observation revealed that there was a black spot on the rat intestine receiving carrageen.

References

1. Utama IH. Fungsi pankreas. Denpasar: Fakultas Kedokteran Hewan Universitas Udayana; 1998.hal.2031.

2. Robins. Pathologic basis of disease. 6th Ed. Sydney: W.B Saunders Co; 1998.p.911-913.

3. Tortora GJ and Anagnostakos NP. Principles of anatomy and physiology. 6th Ed. New York: Harper & Row Publishers; 1990.p.527-530.

4. Ganong, WF. Fisiologi kedokteran. 14th Ed. diterjemahkan oleh: Adrianto P. Jakarta: EGC; 1992. hal.317-325.

5. Suntoro, H. Metode pewarnaan histologi dan histokimia. Jakarta: Bharata Karya Aksara; 1983.hal.42-49.
6. Reynolds JEF. Martindale The pharmacopeia. London: The Pharmaceutical Press, 1982.p.951-952.

7. Handoko T dan Suharto B. Insulin, glukagon, dan antidiabetik oral. Dalam: Ganiswara SG, Editor utama. Farmakologi dan Terapi. Ed. 4. Jakarta: Bagian Farmakologi Universitas Indonesia; 1995.hal.467-481.

8. Laurence dan Bacharach. Evaluation of drug activities pharmacometrics. London: Academic Press; 1964.p.161.

9. KKI Phytomedica. Penapisan farmakologi, pengujian fitokimia, dan pengujian klinik. Jakarta: Yayasan Pengembangan Obat Alam Phytomedica; 1993.hal.16-17.

10. Widowati L. Pengaruh ekstrak biji Trigonella foenum graecum L., terhadap kadar gula darah, glutation dan gambaran kerusakan sel B pankreas pada tikus NIDDM. Jakarta: F-MIPA Universitas Indonesia Prog Studi Kefarmasian; 2003.hal.20-21, 66-73.

11. Chapman VJ and Chapman DJ. Seaweed and their uses. 3rd Ed., London: Chapman and Hall; 1980.p.194-225.

12. Stomach aches caused by carrageenan. diambil dari: http:/www. notmilk.com/carragenan. htm., 2003; diakses 20 September, 2003.

13. Carrageenan may caused stomach aches lesions, cancer. diambil dari: http:/www.ENN.com/cncer.htm, 2003; diakses 20 September 2003.

14. Yu G, Guan H, Ioanviciu A, Sikkander S, Thanawiroon C, Tobachman J, Toida T, Linhardt R. Structural studies on carrageenan derived oligosaccharides. Carbohydrat Research. 2002; 337:433-440.
How to Cite
RAHAYU, Lestahari; DAMAYANTI, Rahma; WIKANTA, Thamrin. Gambaran Histopatologi Pankreas Tikus Hiperglikemia setelah Mengkonsumsi K-Karagenan dan í-Karagenan. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 4, n. 2, p. 96-101, sep. 2006. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/596>. Date accessed: 05 nov. 2024.
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