Isolasi dan Uji Antimikroba Substansi Bioaktif Mikroba Endofit Ranting Tanaman Johar (Cassia siamea Lamk.)

  • Shirly Kumala Universitas Pancasila
  • Syarmalina Syarmalina Universitas Pancasila
  • Atut Ruswita Handayani Universitas Pancasila

Abstract

Assay for secondary metabolite of endophytic fungi from Cassia siamea Lamk.. (Johar Plant) has been investigated. Endophyitic fungi are microorganisms that live asymtomatically within the living tissue of host plants. Endophytic fungi associate with plant, they can help the metabolism of host plant and produce potensial secondary metabolite. The purpose of this experiment were to investigate endophytic microbial agents. Isolate of endophytic fungi from twig of Cassia siamea Lamk., using direct seed method was carried out. The microbial activity was carried out with dilution method using paper disk and glass cylinder. The results showed 3 isolates of bacteria and 7 isolates of endophtyic fungi. The metabolite from three isolates of bacteria had the anti-microbial activity more poten toward Gram positive than Gram negative and four isolates of fungi had anti-Aspergillus agent.

References

1. Syamsuhidayat SS dan Hutapea JR. Inventaris tanaman obat Indonesia. Jakarta:Departemen
Kesehatan Republik Indonesia; 1991.hal.124-125.

2. Wahyudi P. Teknik skrining terhadap mikroba endofit penghasil antibiotika baru. Prosiding temu ilmiah Jaringan Kerjasama Kimia Indonesia, Seminar Nasional II Kimia dalam Pembangunan Jaringan kimia Indonesia; Yogyakarta, 5-6 Mei 1998.hal.316-25

3. Carrol GC. Fungal endophytes in stems and leaves from latent pathogent mutualistic symbiont. Ecology. 1988;69:2-9

4. Saikkonen K, Faeth SH, Helander M, Sullivan TJ. A continuum of interactions with host plants. Fungal Endophytes 1998;319. diambil dari: http:// phyto.annualreviews.Org/cgi/content/abstract29. diakses 26 November, 1999.

5. Petrini, Siebeer OTN, Toti L, Viret 0. Ecology, metabolic, production, and substrate utilization in endophytic fungi. Natural toxin. 1992;1:185-96.

6. Bacon CW. Procedure for isolating the endophytes from fall rescue and screening isolates for ergot alkaloid. Appl Environ Microbial. 1988;54:2615-18.

7. Hadioetomo, Sirirogi R. Dasar dalam praktek, teknik dan prosedur dasar. Jakarta: Gramedia; 1985.hal.60 104.

8. Lay BW. Analisa mikroba di laboratorium. Jakarta: PT. Raja Grafindo Persada; 1994. hal. 48-49, 115-17.

9. NCCLS methode for dilution antimicrobial suscettibility test for bacteria that grow aerobically. 1990;10(8):1-31.

10. Rosane Y, Ibrahim F, Susilo J dan Sukara E. Isolasi dan seleksi jamur endofit pada tanaman belimbing wuluh (Averhoa bilimbi L.) yang menghasilkan antibiotik. Jurnal Mikologi Indonesia. 2001;1(2):13439.
How to Cite
KUMALA, Shirly; SYARMALINA, Syarmalina; HANDAYANI, Atut Ruswita. Isolasi dan Uji Antimikroba Substansi Bioaktif Mikroba Endofit Ranting Tanaman Johar (Cassia siamea Lamk.). JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 4, n. 1, p. 8-14, apr. 2006. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/598>. Date accessed: 26 apr. 2024.
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