Teknik Isolasi Protoplasma Kapang Trichoderma ssp. Menggunakan Enzim Lisis

  • Priyo Wahyudi P3 Teknologi Bioindustri-BPPT
  • Zainal Abidin FMIPA Departemen Biologi Universitas Indonesia
  • Retno Lestari FMIPA Departemen Biologi Universitas Indonesia

Abstract

Trichoderma is well known industrial fungi producing many kinds of industrial enzymes and as biological control agent. The applications of modern biotechnology using Trichoderma or its derived metabolites. Considering to the variety of the species of Trichoderma and enzymes or metabolites produced by Trichoderma lead to create a fusan of Trichoderma. The first step toward protoplast fussion is protoplasm isolation. In this experiment has been conducted protoplasm isolation of Trichoderma harzianum, T. viride, T. aureoviride and T. pseudokningii using lysing enzyme in room temperature for 1- 5 hours incubation. The aim of this experiment is to find the best condition and time of incubation in producing protoplast optimally. Trichoderma mycelia (1 mg) sink in osmotic stabilizer solution (pH 5.8), crushed and add 1 ml lysing enzyme 2%, then incubate in shaker incubator (room temperature) for 5 hours. Observation of the protoplast is conducted using haemacytometer for 1 until 5 hours incubation. After 5 hours incubation in room temperature showed the T. harzianum protoplast is the highest number of protoplast (40,150 protoplast/ml), followed by T. pseudokoningii (10,375 protoplas/ ml), T. viride (15,075 protoplast/ml) and T. aureoviride (10,050 protoplas/ml).

References

1. Rao NSS. Mikroorganisme tanah dan pertumbuhan tanaman. 2nd ed. Jakarta: UI Press, 1994. hal. 353. Zaldivar M. 2001.

2. Trichoderma aureoviride 7-121 a mutant with enhanced production and/or biocontrol. 9hlm. diambil dari http://www.ejbiotechnology.info/ content/vol 14/issue3full/7index.html. diakses 23 Juli, 2003.

3. Suwahyono U & P Wahyudi. Trichoderma harzianum dan aplikasinya: Penelitian dan pengembangan agen pengendali hayati. Jakarta: Direktorat Teknologi Bioindustri BPPT; 2001.hal.5.

4. Tripanji, S. Produksi kitinase dan selulase dari Trichoderma harzianum menggunakan lendir biji kakao sebagai medium pertumbuhan. Yogyakarta: Prosiding seminar nasional kimia UGM; 1999.hal. 150-154.

5. Pederby JF & S Isaac . An Improved procedure for protoplast isolation from Aspergillus nidulans.
Mikrobiol letter. 1976; 7-9.

6. Cocking EC. 1999. Plant protoplast. 8 hlm. diambil dari http://www.uio.no/conferences/imc7/NFotm2000.htm. diakses 23 juli, 2003.

7. Labeda DP. Isolation of biotechnological organism from nature. New York: Mc Graw-Hill Pub Co; 1990. hal.322.

8. Ogawa KH, Toyama & N Toyama. Degradation of fungal cell walls and protoplast formation by a mycolytic enzyme produce by Trichoderma viride. Myuzaki daigulan nogakubi: 1979; hal.387-198.

9. Bennitez T, TG. Villa, & IG. Acha. Protoplast from Trichoderma viride. Arch Microbiol. 1975.hal.199-203.

10. Adil EIM. Penuntun praktikum fisiologi hewan. Depok: Biologi-FMIPA UI; 2002.hal.44.

11. Gams W & J Bisset. Morphologi and identification of Trichoderma. Dalam: Kubicek CP & G.E Harman (ed). Trichoderma, Gliocladium. Basic biology, taxonomy and genetic. London: Taylor & Francis ltd; 1998.hal.169-175.

12. Muslimin LW. Mikrobiologi lingkungan. Jakarta: Unhas PPPSL Dirjen Dikti Departmen P & K;1995.hal.173.
How to Cite
WAHYUDI, Priyo; ABIDIN, Zainal; LESTARI, Retno. Teknik Isolasi Protoplasma Kapang Trichoderma ssp. Menggunakan Enzim Lisis. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 4, n. 1, p. 19-24, apr. 2006. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/600>. Date accessed: 05 nov. 2024.
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