Identifikasi, Uji Antioksidan, dan Uji Toksisitas Senyawa Bioaktif dalam Caulerpa sertularioides (Vahl.) C. Agard.

  • Thamrin Wikanta Pusat Riset Pengolahan Produk dan Sosial Ekonomi Kelautan
  • Swasono R. Tamat Pusat Radioisotop dan Radiofarmaka BATAN
  • Magdalena Sinta Maryati Universitas Pancasila

Abstract

Caulerpa sertularioides (Vahl.) C. Agard. is a green seaweed species in the Caulerpaceae family which has been used for medication. Extraction of bioactive compounds from Caulerpa sertularioides have been carried out and screened based on the antioxidative property against DPPH and on toxicity test by the brine shrimp lethality test (BSLT) method. Phytochemical screening of the methanol extract indicated the presence of a triterpen. The methanol extract was partitioned in sequence with n-hexanewater (1:1) and chloroform-water (1:1). The IC50 value of the water extract was found 327 ug/ml and the LCs value was 115 ug/ml as compared to the ICs of vitamin C positive control of 21 ug/ml. Fractination of the water extract in a silica gel column with various ratio of chloroform-methanol did not produce distinct separation. Fractions were analyzed on TLC and fractions of similar components were collected. Fraction I has IC50 value of 326 ug/ml and LC50 value of 68 ug/ml. Further separation and purification of fraction I on a preparative thin layer chromatographic system using chloroform-methanol (50:1) produced five isolates bearing antioxidative activity. The third isolate that showed the strongest activity, when analyzed using a GC-MS system indicating seven compounds, but only [(2-fluorophenyl)methyl)-1H-purin-6-amine that may have an antioxidative activity.

References

1. Suhartini S. Penapisan awal Caulerpa racemosa, Sesuvium portulacastrum, Xylocarpus granatum dan Ulva lactuca sebagai antimikroba [skripsi]. Bogor: Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor; 2003.hal.1-5.

2. Jana A. Teknologi produk perikanan dalam industri farmasi: potensi dan pemanfaatan makro-algae laut Bogor. Jakarta: Tim Rumput Laut Badan Pengkajian dan Penerapan Teknologi; 1993.hal.1, 5, 7.

3. Darusman LK, Sajuthi D, Sutriah K dan Pamungkas D. Ekstraksi komponen bioaktif sebagai bahan obat dari kerang, bunga karang dan ganggang di perairan pulau pari, kepulauan seribu. Bogor: Institut Pertanian Bogor; 1992.hal. 8.

4. Atmadja WS, Kadi A, Sulistijo, Satari R, editors. Pengenalan jenis-jenis rumput laut Indonesia. Jakarta: Puslitbang Oseanologi-LIPI; 1994.hal.21, 158.
5. Fenical W. Diterpenoid dalam produk alami lautan dari segi kimiawi dan biologi. Jilid II. Diterjemahkan oleh PJ. Scheur. Semarang: IKIP Semarang Press; 1995.hal.177-8.

6. Sri ER. Pemanfaatan makroalga oleh masyarakat di teluk pedada, padang cermin, Lampung Selatan: Majalah Ilmiah Widya. 2000; XVII (181): 42-8.

7. Hill MN. The seas ideas and obseryation on progress in the study of the seas. Vol 2. New York: Interscience Publisher; 1963.p.183-7.

8. Lembaga Oseanologi - Lembaga Ilmu Pengetahuan Indonesia. Rumput laut (algae): manfaat, potensi dan usaha budi-dayanya, 1978.hal.7.

9. Yen GC, Chen HY. Antioxidant and pro-oxidant effects of various tea extracts. J Agric Food Chem. 1997; 30

10. Yen GC, Chen HY. Antioxidant activity of various tea ekstract in relation to their antimutagenicity. JAgric
Food Chem. 1995;27-32.

11. Anderson JE, Mc Laughlin. A blind comparison of simple bench-top bioassays and human tumor cell cytotoxicities studies as antitumor prescreens phyto chemical analysis. 1991;2:107-11.

12. Windono T, Soedirman S, Yudawati U, Ermawati E, Srielita A, Erawati TI. Uji peredaman radikal bebas terhadap 1,1-diphenyl-2-pikrilhidrazyl (DPPH) dari ekstrak kulit buah dan biji anggur (Vitis vinifera L). Artocarpus. 2001;34-43.

13. Mayer BN. Brine shrimp: a convenient general bioassay for active plant constituents. Planta Medica.
1982;31-4.

14. Colegate SM, RJ Molyneux. Bioactive natural products, detection, isolation and structural determination. London: CRC Press, Boca Raton Ann Arbor; 1993.p.442-54.

15. Ketaren S. Pengantar minyak dan lemak pangan. Jakarta: Universitas Indonesia Press, 1986.hal.45-9.

16. Alam G. Brine shrimp lethality test (BSLT) sebagai bioassay dalam isolasi senyawa bioaktif dari bahan alam. Majalah Farmasi dan Farmakologi. 2002; 423-5.
How to Cite
WIKANTA, Thamrin; TAMAT, Swasono R.; MARYATI, Magdalena Sinta. Identifikasi, Uji Antioksidan, dan Uji Toksisitas Senyawa Bioaktif dalam Caulerpa sertularioides (Vahl.) C. Agard.. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 4, n. 1, p. 25-32, apr. 2006. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/601>. Date accessed: 22 dec. 2024.
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