Uji Sitotoksik Ekstrak Buah Phaleria macrocarpa terhadap Sel Kanker Lestari A 549 dan THP-1
Abstract
Fruits of Mahkota Dewa (Phaleria macrocarpa (Scheff.) Boerl.) has been used to cure various health problems, including empirical treatment for cancer. The therapeutic effect of natural material is related to the chemical compound contains. In fruits of Phaleria macrocarpa, various chemicals are present such as alcaloid, flavonoid saponin, fenol and tannin, which showed anticancer activity, as well as unknown compounds which ones presumably supportive for cancer treatment. An in vitro experiment is therefore set up with the objective of examining the effect of fruit extract on male human lung carcinoma epithelial cells (A-549 cell line) and human peripheral blood leukemia acute cells (THP-1 cell line). Various concentrations: 5, 10, 25, 50 and 100 ppm of fruit extract were evaluated. Observations were made 72 hour after incubating A-549 cells and THP-1 cells in each treatments. The results showed that fruit might inhibit the growth of A-549 cells and THP-1 cells. Inhibitory concentration (LC) of fruit extract after 72 hours of incubation is 9,43 ppm for A-549 cells and 5,36 ppm for THP-1 cells. Inhibitory potential effect of these fruits in THP-1 cells are better than in A-549 cells. The effect might be associated to the active compounds contained in these material.
References
2001.hal.31-35.
2. Gotama IBI, Sugiarto S, Nurhadi M, Widiyastuti Y, Wahyono S, Prapti IJ. Inventaris tanaman obat Indonesia. Jilid V. Jakarta: Departemen Kesehatan. Badan Penelitian dan Pengembangan Kesehatan; 1999.hal.147-148.
3. de Padua LS, Bunyapraphatsara N, and Lemmens RHMS. Plant Resources of South East Asia No 12(1). Medical and Poisonous Plants 1. Printed in Bogor Indonesia (PROSEA). Leiden: Backhuys Publishers; 1999.hal.36-48.
4. Willaman JJ. Some biological effects of the Flavonoids. J of the American Pharmaceutical Assoc.
1995; 44:404-409.
5. Hartati MSW, Wahyuono S, Artama WT. Efek sitotoksisitas okandrin, senyawa bioaktif hasil isolasi dari daun Nerium indicum Mill. terhadap sel Myeloma. Berkala Ilmu Kedokteran. 2000;32(4).
6. Live Cell Imaging Cell Motility. diambil dari http:// www.microscopyu.com/moviegallery/livecelli-maging. diakses 6 Desember, 2005.
7. Human, peripheral blood, leukemia acute monocytic. diambil dari http://www.biotech.ist.unige.it/cldb/
c14515.html THP-1. diakses 6 Desember, 2005.
8. Buku Panduan Teknologi Ekstrak, Dirjen POM, Dep kes RI; 2000.
9. Meyer BN. Brine shrimp: a convinient general bioassay for active plant constituent. Planta Medica. 1982;45:31-4.
10. Finney DJ. Prebit analysis, 3rd ed. New Delhi: Cambridge University Press;1971.p.333.
11. Triana Hertiani & Silvia Utami TP. Uji toksisitas kulit batang Mahkota dewa (Phaleria macrocarpa (Scheff.) Boerl.) terhadap Artemia salina Leach dan Profil Kromatografi Lapis Tipis Fraksi Aktif. Majalah Farmasi Indonesia. 2002; hal. 65-70.
12. Sumastuti R dan Sonlimar M. Efek sitotoksik ekstrak buah dan daun Mahkota Dewa (Phaleria macrocarpa (Scheff) Boerl.) terhadap sel HeLa. diambil dari http://www.tempo.co.id/medika/arsip/122002/art-3. htm. diakses 7 Desember, 2005.
Licencing
All articles in Jurnal Ilmu Kefarmasian Indonesia are an open-access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which permits unrestricted non-commercial used, distribution and reproduction in any medium.
This licence applies to Author(s) and Public Reader means that the users mays :
- SHARE:
copy and redistribute the article in any medium or format - ADAPT:
remix, transform, and build upon the article (eg.: to produce a new research work and, possibly, a new publication) - ALIKE:
If you remix, transform, or build upon the article, you must distribute your contributions under the same license as the original. - NO ADDITIONAL RESTRICTIONS:
You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
It does however mean that when you use it you must:
- ATTRIBUTION: You must give appropriate credit to both the Author(s) and the journal, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
You may not:
- NONCOMMERCIAL: You may not use the article for commercial purposes.
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.