Kapang Endofitik Tanaman Brucea javanica (L.) Merr yang Berpotensi menghasilkan Enzim Xilanase

  • Wibowo Mangunwardoyo FMIPA Departemen Biologi Universitas Indonesia
  • Shirly Kumala Universitas Pancasila
  • I. G.A. Ayu Wiratih Universitas Pancasila

Abstract

Xylanase enzyme activity of endophytic fungi from Brucea javanica (L.) Merr has been done. Fruit, leave and stem of the plant collected from Bogor, Cianjur, and Tawangmangu which used in this study. To evaluate the enzyme activity the endophytic fungi isolate were inoculated on PDY medium and fermented at room temperature, shake at 150 rpm for 14 days. The supernatan were used to examine the extracellular enzyme and the biomass for intracellular enzyme. The DNS methods was used to test the enzyme activity. The results showed isolate 1.1.8; 1.2.17; 1.3.16 and 1.3.6 produced intra and extracellular xylanase enzyme. Isolate 1.1.6 produced only extracellular enzyme while isolate 1.2.2 did not produce both intra and extracellular enzyme.

References

1. diambil dari http://www.menuaiberkahdarihutan/ mikroba.htm.

2. Wahyudi P. Teknik skrining terhadap mikroba endofitik penghasil antibiotik baru. Prosiding Temu Ilmiah Jaringan Kerjasama Kimia Indonesia, Yogyakarta: 1998.hal.316-25.

3. Qureshy AF, Khan LA, Khana S. Expression of Bacillus circulans Teri-42 xylanases gene in Bacillus subtilis. Enzyme and Microbial Technology. 2000;27:
227-33.

4. Pelczar MJ, Chan ECS. Dasar-dasar Mikrobiologi. Alih bahasa Hadioetomo, Jakarta: UI-Press; 1986.hal.189-209.

5. Rani DS, Nand K, Production of thermostable cellulase-free xilanase by Clostridium absonum CFR
- 702. Process Biochemistry. 2000;(36): 355-362.

6. Petrini O, Sieber, Thomas N, Toti L, Viret O., Ecology, Metabolite production and substrate utilization in endophytic fungi. Natural Toxins. 1992;(1):185-196.

7. Caroll CG. Fungal endophytes in stem and leaves from latent pathogent mutualistic symbiont. Ecology. 1988; 69:2-9.

8. Kumala S, Utji R, Sudarmono P and Kardono LBS Isolation of endophytic fungi from Brucea javanica L (Merr) and cytotoxic evaluation of their n-butanol extract from fermentation broth. Pak Journal of Biological Sciences. 2006;9(5): 825-832.

9. Rachman A. Pengantar teknologi fermentasi. Bogor: Pusat Antar Universitas Pangan dan Gizi IPB; 1989. hal. 90-95.

10. Rachman A. Teknologi fermentasi. Bogor: Pusat Antar Universitas Bioteknologi IPB; 1990. hal. 3,4, 162, 163, 198-226.

11. Baraznenok VA, Becker EG, Ankudimova NV, Okunev NN. Characterization of neutral xylanases from Chaetomium cellulolyticum and their biobleaching effect on eucalyptus pulp. Enzyme and Microbial Technology; 1999, 25:651-59.

12. Judoamidjojo RM, Darwis AA, Said EG. Teknologi fermentasi. Bogor: PAU Bioteknologi IPB; 1992. hal.
37-51,111-13.

13. Juniarti DH. Isolasi mikroba endofitik dari ranting tumbuhan trengguli (Cassia fistula L) dan aktivitasnya ensim xylanase [Skripsi]. Jakarta: Fakultas Farmasi Universitas Pancasila; 2004.
How to Cite
MANGUNWARDOYO, Wibowo; KUMALA, Shirly; AYU WIRATIH, I. G.A.. Kapang Endofitik Tanaman Brucea javanica (L.) Merr yang Berpotensi menghasilkan Enzim Xilanase. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 4, n. 1, p. 38-41, apr. 2006. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/603>. Date accessed: 03 july 2024.
Citation Formats
Issue
Vol 4 No 1 (2006): JIFI
Section
Articles

Licencing
All articles in Jurnal Ilmu Kefarmasian Indonesia are an open-access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which permits unrestricted non-commercial used, distribution and reproduction in any medium.

This licence applies to Author(s) and Public Reader means that the users mays :

  • SHARE:
    copy and redistribute the article in any medium or format
  • ADAPT:
    remix, transform, and build upon the article (eg.: to produce a new research work and, possibly, a new publication)
  • ALIKE:
    If you remix, transform, or build upon the article, you must distribute your contributions under the same license as the original.
  • NO ADDITIONAL RESTRICTIONS:
    You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

It does however mean that when you use it you must:

  • ATTRIBUTION: You must give appropriate credit to both the Author(s) and the journal, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.

You may not:

  • NONCOMMERCIAL: You may not use the article for commercial purposes.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.