Fermentasi Diam dan Goyang Isolat Kapang Endofit dari Brucea javanica L. Merr. dan Uji Aktivitas Antimikroba

  • Shirly Kumala Universitas Pancasila
  • Wibowo Mangunwardoyo FMIPA Departemen Biologi Universitas Indonesia
  • Peny Budiarti Universitas Pancasila

Abstract

Exploring an antibiotic from microorganism is potential to discover the new antibiotic. The endophytic microorganism is one of the minimanufacturer producing the secondary metabolite such as antibiotic. Screening on the endophytic molds isolated from Brucea javanica L. Merr has been carried out. Fermentation in still and shake culture were used to produce the antibiotic using PDY medium. Antimicrobial activity was assayed using diffusion agar method. The results revealed that shake culture gave a better antimicrobial activity than still culture. Amount of eight isolate studied showed microbial activity against Gram positive bacteria: Staphylococcus aureus and Bacillus subtilis also Gram Negative bacteria: Escherichia coli and Salmonella typhi, however no microbial activity recorded againts Candida albicans. Isolate number 1.2 gave the highest antimicrobial activity on still culture and isolate number 1.7 gave the highest antimicrobial activity on shake culture. Further research should be carried out on identification and purification of the antimicrobial agent.

References

1. Wahyudi P. Teknik terhadap mikroba endofitik penghasil antibiotika baru. Prosiding Temu Ilmiah Jaringan Kerjasama Kimia Indonesia. Seminar Nasional II Kimia Dalam Pembangunan Jaringan Kerjasama Kimia Indonesia, Yogyakarta 5-6 Mei, 1998: 316-328.

2. Petrini O, Sieber TN, L Toti, D Viret. Ecologi metabolic production, and substrate utilization in endophytic fungi. Natural Toxin. 1992; 1:185-196.

3. Soedibyo M. Alam sumber kesehatan, manfaat dan kegunaan. Jakarta: Balai Pustaka; 1998. hal. 95-6.

4. Tomita F. Screening of useful strains in international. post graduate university courses in microbiology japanese national commision for UNESCO; 1985.p. 221-32.

5. Miles CO. Endophytes fungi indigenous australian grasses. Associated with Toxicity of Livestock Applied and Environmental Microbiology; 1998.p.601-6.

6. Pelczar MJ, Chan ECS. Mikrobiologi 2. Diterjemahkan oleh Hadioetomo R.S., Imas T, Tjitrosomo S.S. Agra S.L. Jakarta: Penerbit Universitas Indonesia; 1986. hal.523-5.

7. Liesbetini H. Teknologi fermentasi. Bogor: Pusat Antar Universitas Bioteknologi IPB; 1990. hal.3-4;
198-226.

8. Pelczar MJ, Chan ECS. Mikrobiologi 1. Diterjemahkan oleh Hadioetomo R. S, Imas T, Tjitrosomo SS, Agra SL, Jakarta: Penerbit Universitas Indonesia; 1986. hal.188-99.
How to Cite
KUMALA, Shirly; MANGUNWARDOYO, Wibowo; BUDIARTI, Peny. Fermentasi Diam dan Goyang Isolat Kapang Endofit dari Brucea javanica L. Merr. dan Uji Aktivitas Antimikroba. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 3, n. 2, p. 60-63, sep. 2005. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/608>. Date accessed: 24 nov. 2024.
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Vol 3 No 2 (2005): JIFI
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