Formulation and Evaluation of Mouthwash with 1,5-Bis(3’-EToksi-4’-Hidroksifenil)-1,4-Pentadien-3-on as Antimicrobial Ingredient

  • Esti Mumpuni Fakultas Farmasi Universitas Pancasila
  • Agus Purwanggana Universitas Pancasila
  • Esti Mulatsari Universitas Pancasila
  • Ryan Pratama Universitas Pancasila

Abstract

1,5-bis (3-ethoxy-4-hydroxyphenyl) -1,4-pentadien-3-one (EHP) compound is an analogous compound of curcumin which has antimicrobial activity. In this study, the formulation and evaluation of mouthwash was carried out with EHP as an antimicrobial ingredient. The formulation of mouthwash was made with various concentrations of EHP 3, 6, 12, 16 and 18 ppm. Evaluation of mouthwash included organoleptic test, pH, clarity, density and antimicrobial activity against Staphylococcus aureus, Steptococcus mutans, and Candida albicans. Antimicrobial test was done by agar diffusion method. The results of organoleptic test obtained mouthwash was clear blue liquid, the aroma of mint, and fresh cold taste; pH range of 6.15 - 6.74; density of 1.0419 - 1.0561 g/cm3. The evaluation of mouthwash showed that the mouthwash was stable in storage for 1 month at 40 °C. Antimicrobial tests showed the diameter of inhibitory zone against Staphylococcus aureus (ATCC 6538); Streptococcus mutans (ATCC 31987) and Candida albicans (ATCC 10231) ranged from 6.2 to 8.4 mm at the concentration of EHP 18 bpj. The results showed that EHP compound was potential as antimicrobial ingredient in moutwash formula.

References

1. Mumpuni E, Indriana P, Sulastri E, Rusnawan E. Synthesis and antioxidant test of 1.5-bis (3'ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one (EHP). JIFI. 2010. 8:91-100.

2. Mumpuni, E., Rahayu, L., Nurrochmad, A. Toksisitas dan Antiinflamasi Senyawa 1,5-bis(3’-etoksi-4’-hidroksifenil)-1,4-pentadien-3-on, JIFI . 2015. 13(1) : 45- 9

3. Purwanggana A, Mumpuni E, Mulatsari E. In vitro and In silico antibacterial activity of 1,5-bis(3’-ethoxy-4’-hydroxyphenyl)-1,4-pentadiene-3-one. Int J. Pham Pham Sci. 2018. (10) 5 : 71 – 6.

4. Krespi Y P, Shrime M G, and Kacker A. The relationship between oral malodor and volatile sulfur compound-producing bacteria. American Journal Otolaryngology-Head and Neck Surgery. 2006. https://doi.org/10.1016/j.otohns.2005.09.036

5. Komariah A. Staphylococcus aureus ( in vitro ) Antibacterial Activity of Nano Chitosan on Staphylococcus aureus. Seminar Nasional XI Pendidikan Biologi FKIP UNS Biologi , Sains , Lingkungan , dan Pembelajarannya. Surakarta 1 November 2015. 12 (1): 371– 7.

6. Jawetz, Melnick, Adelberg. Mikrobiologi Kedokteran edisi 20. Alih Bahasa Nugroho E, Maulany RF. Jakarta: Penerbit Buku Kedokteran 1986. Hal 153-7, 627-8.

7. Power, J. M., dan Sakaguchi, R. I. Craig’s Dental Materials 12th edition. 2006. Mosby Elsevier, St. Louis.p.

8. Akande, et al. Efficacy of Diferent Brands of Mouthwash Rinses on Oral Bacterial Loud Count in Healty Adults. African Journal of Biomedical Research. 2004. 7 : 125 - 8

9. Phatak A.A, Patankar R.D, Galgatte U.C, et al. Antimicrobial activity of a poly-herbal extact against dental micro flora. Res J Pharm Bio Chem Sci. 2011. 2 (2) : 533 – 9.

10. Banu J.N, and Gayathri V. Preparation of Antibacterial herbal mouthwash against oral pathogens. Int J Curr Microbiol App Sci .2016. 5(11) : 205 – 21.

11. Paula I.M, Moraes F.C, Souza O.V, and Yamamoto C.H. Development of mouthwash with rosmarinus officinalis extract. Brazilian J Pharm Sci. 2014. 50 (4) : 851 – 8.

12. Kono S.R, Yamlean P.V.Y, dan Sudewi S. Formulasi larutan pencuci mulut herba patikan kebo (Euphorbia hirta) dan uji antibakteri Prophyromonas gingivalis. Pharmacon Jurnal Ilmiah Farmasi-Unsrat. 2018. 7 (1) : 37-46.

13. Venancio G.N, et al. Herbal mouthwash based on libidibia ferrea : microbiological control, sensory characteristics, sedimentation, pH and density. Rev Odontol unesp 2015. 44 (2) :118 – 24.

14. Departemen Kesehatan Republik Indonesia. Farmakope Indonesia. Edisi IV. Jakarta: Direktorat Jendral Pengawasan Obat dan Makanan; 1995. H.63-4, 529-0, 756-7, 998, 1039.

15. Hudzicki J. Kirby-Bauer disk diffusion susceptibility test protocol. 2009. American Society for Microbiology.
Published
2019-04-24
How to Cite
MUMPUNI, Esti et al. Formulation and Evaluation of Mouthwash with 1,5-Bis(3’-EToksi-4’-Hidroksifenil)-1,4-Pentadien-3-on as Antimicrobial Ingredient. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 17, n. 1, p. 87-94, apr. 2019. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/615>. Date accessed: 17 july 2019.
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Articles