Antidiarrheal Effect of Water Extract of The Nagasari Flowers (Mesua ferrea L.) in BALB/c Mice Induced by Castor Oil

  • Putu Monik Ananta Puspitarini Universitas Mataram
  • Iman Surya Pratama Universitas Mataram
  • Bambang Fajar Suryadi Universitas Mataram


Nagasari (Mesua ferrea L.) has a empirically repute for management of diarrhea in West Nusa Tenggara Province. However, the safety and efficacy of the nagasari flowers extract have not been scientifically validated in animal model. This study wasaimed to investigate the antidiarrheal effect of water extract of the nagasari flowers in BALB/c mice. The antidiarrhea activity of nagasari flowerswas investigated using castor oil-induced diarrhea increase in diarrhea index in mice.In castor oil-induced diarrhea test, mice received various consentrations of nagasari flowers extract (0,4% w/v; 0,8% w/v; and 1,6% w/v), where as negative controls received CMC-Na 1% and positive controls received loperamide 0,7 mg/kgBW orally once daily for 1 day and the mice droppings were observed. The phytochemical screening of nagasari flowers contain flavonoid, saponin, and phenolic. Water extract of nagasari flowers at concentration 1,6% showed significant inhibitory activity against castor oil-induced diarrhea when compared with positive controls (diarrhea index 0,083 ; p>0,05). Therefore, this study provides a scientific support for the acclaimed traditional use water extract of Mesua ferrea L. for the treatment of diarrheal diseases.


1. Tripathi, K. D. Essentials of Medical Pharmacology. Edisi ke-7, India : Jaypee Brothers Medical Publishers. 2013. p. 677.

2. Dinas Kesehatan Provinsi NTB. Profil Kesehatan Provinsi Nusa Tenggara Barat. NTB : Dinas Kesehatan Provinsi NTB. 2015.

3. Kementerian Kesehatan Republik Indonesia. Data dan Informasi Kesehatan Indonesia 2017. Jakarta : Kementerian Kesehatan Republik Indonesia. 2018.

4. World Health Organization. The Rational Use of Drugs in the Management of Acute Diarrhoea in Children.
Geneva : World Health Organization. 1990.

5. Listiawati, N. P., Juniawan, I. B. H., Anom, A. A. I. A., Sudarsana, I. W. G., and Wibawa, I. G. J. S. Dharma Usadha Kuranta Bolong Klimosadha Sang Hyang Klimosadha. Diterjemahkan oleh Listiawati, N. P., Juniawan, I. B. H., Anom, A. A. I. A., Sudarsana, I. W. G., Wibawa, I. G. J. S. Mataram : STAHN Gde Pudja Mataram. 2016.

6. Mazumder, R., Dasridar, S. G., Basu, S. P., Mazumder, A., and Singh, S. K. Antibacterial Potentiality of Mesua ferrea Linn. Flowers. Phytotherapy Research. 2004. 18 : 824-26.

7. Gul, R., Jan, S. U., Faridullah, S., Sherani, S., and Jahan, N. Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan. The Scientific World Journal.2017. 2017 : 1-7.

8. Hui, H., Chen, M., Li, G., Feng, H., et al. Andrographolide Attenuates Senna- and Castor Oil-induced Diarrhea in Mice. Latin American Journal of Pharmacy. 2013. 32(8): 1113-7.

9. Han, X., Pang, Y., Liu, S., Tan, Z., Tang, S., Zhou, C., et al. Antidiarrhea and Antioxidant Activities of Honokiol Extract from Magnoliae officinalis Cortex in Mice. Tropical Journal of Pharmaceutical Research. 2014. 13 (10): 1643-51.

10. Yu, J., Zhang, Y., Song, X., Yang, Y., Jia, R., Chen, X, et al. Effect of Modified Pulsatilla Powder on Enterotoxigenic Escherichia coli O101-Induced Diarrhea in Mice. Hindawi. 2017. 2017: 11.

11. Sahu, A. N., Hemalatha, S., and Sairam, K. Quality Control Studies of Mesua ferreaLiin. Flowers. International Journal of Herbal Medicine.2013. 1 (2):124-30.

12. Tunaru, S., Althoff, T. F., Nusing, R. M., Diener, M., and Offermanns, S. Castor Oil Induces Laxation and Uterus Contraction Via Ricinoleic Acid Activating Prostaglanding EP3 Receptors. Proceedings of the National Academy of Science of the United States of America. 2012. 109 (23): 9179-84.

13. Maniyar, Y., Bhixavatimath, P., and Agashikar, N. V. Antidiarrheal Activity of Flower of Ixora coccinea Linn. In Rats. Journal of Ayurveda and Integrative Medicine. 2010. 1 (4): 287-91.

14. Tiwari, P. K., Irchhaiya, R., and Jain, S. K. Evaluation of Anticonvulsant Activity of Mesua ferrea Liin. Ethanolic Flower Extract. International Journal of Phamacy and Life Sciences, 2012. 3 (3): 1507-08.
How to Cite
PUSPITARINI, Putu Monik Ananta; PRATAMA, Iman Surya; SURYADI, Bambang Fajar. Antidiarrheal Effect of Water Extract of The Nagasari Flowers (Mesua ferrea L.) in BALB/c Mice Induced by Castor Oil. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 17, n. 1, p. 68-71, apr. 2019. ISSN 2614-6495. Available at: <>. Date accessed: 19 may 2024. doi: