Efek Imunostimulan Ekstrak Etanolik Herba Pegagan (Centella asiatica (L.) Urban) terhadap IgG Mencit Jantan

  • Shirly Kumala Universitas Pancasila
  • Aulia Tisna Dewi Universitas Pancasila
  • Yun Astuti Nugroho Badan Penelitian dan Pengembangan Farmasi

Abstract

The immunostimulating effect of an ethanolic extract of Pegagan herbs to male mice has been determined. Thirty male mice divided into five groups (P1-P5) were induced by 1% sheep red cells for 14 days. P1, P2 and P3 were treated with three doses of an ethanolic extract 4.124 mg/20g BW, 8.25 mg/20g BW and 16.5mg/20gBW respectively. P4 was a positive control using Stimuno 0.55 mg/20g BW, and P5 was a negative control using distilled water Blood samples were taken at day 1, 8 and 15, and the concentration of IgG was measured by Radial Immunodiffusion method. A two way Anova showed no significant difference between P1, P2, and P5, but P3 was higher than P4. The obtained results showed that the ethanolic extract of 16,5 mg has a potent immunostimulating effect.

References

1. Hargono D. Sekelumit mengenai obat nabati dan sistem imunitas. Cermin Dunia Kedokteran; 1996. hal. 108-59.

2. Tjokronegoro A. Peranan mikrooganisme dan komponennya sebagai imunomodulator sistem imun tubuh manusia. Majalah Kedokteran Indonesia. 1990;40(11):613-19.

3. Bratawidjaja KG. Imunologi dasar. Jakarta: Fakultas Kedokteran Universitas Indonesia; 2002. hal. 119
25.

4. Subowo. Efek imunomodulator dari tumbuhan obat. Warta Tumbuhan Obat Indonesia. 1996.3(1):1-4.

5. Winarto WP. Khasiat dan manfaat pegagan tanaman penambah daya ingat. Edisi I. Jakarta: Agromedia Pustaka; 2003. hal. 6-10.

6. Hargono D, Sumali W. Cara pembuatan ekstrak. Jakarta: Depkes RI; 1986. hal. 9-10.

7. Kabat EA. The reagent of immunology detection of antigen-antibody interaction: structural concept in immunology and imunochemistry. New York: Holt Rinehart Winston Inc; 1986. p. 27-40.

8. Nisonoff A. Immunologycal assays and purification and antibodies, introduction to molecular immunology. Massachusett: Sinaeur Ass Inc.; 1982. p. 141-45.

9. Schefler WC. Statistik untuk biologi, farmasi, kedokteran, dan ilmu yang bertautan. Terbitan kedua. Diterjemahkan oleh Suroso. Bandung: Institut Teknologi Bandung-Press, 1978. hal. 107.

10. Usman H, Akbar PS. Pengantar statistik. Jakarta: Bumi Aksara; 1985. hal. 271-72.

11. Garvey JS, NA Cremer, DH Sussdorf. Cellular antigens: methods in immunology. 3rded. New York: Addison Wesley Publishing Co Inc; 1977.p. 347-71.

12. Departemen Kesehatan Republik Indonesia. Tinjauan hasil penelitian obat di berbagai institusi. Edisi II. Jakarta: Pusat Penelitian dan Pengembangan Farmasi; 1995. hal. 21-23.

13. Wagner H. Immunostimulants of fungi and higher plants, definition scope aims stimulants; 1984. p. 26-32.

14. Syamsuhidayat SS, Hutapea JR. Inventaris tanaman obat Indonesia (I). Jakarta: Badan Penelitian dan Pengembangan Kesehatan Departemen Kesehatan RI;1991.hal. 132.
Published
2004-09-24
How to Cite
KUMALA, Shirly; DEWI, Aulia Tisna; NUGROHO, Yun Astuti. Efek Imunostimulan Ekstrak Etanolik Herba Pegagan (Centella asiatica (L.) Urban) terhadap IgG Mencit Jantan. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 2, n. 2, p. 53-58, sep. 2004. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/627>. Date accessed: 26 apr. 2024.
Citation Formats
Issue
Vol 2 No 2 (2004): JIFI
Section
Articles

Licencing
All articles in Jurnal Ilmu Kefarmasian Indonesia are an open-access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which permits unrestricted non-commercial used, distribution and reproduction in any medium.

This licence applies to Author(s) and Public Reader means that the users mays :

  • SHARE:
    copy and redistribute the article in any medium or format
  • ADAPT:
    remix, transform, and build upon the article (eg.: to produce a new research work and, possibly, a new publication)
  • ALIKE:
    If you remix, transform, or build upon the article, you must distribute your contributions under the same license as the original.
  • NO ADDITIONAL RESTRICTIONS:
    You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

It does however mean that when you use it you must:

  • ATTRIBUTION: You must give appropriate credit to both the Author(s) and the journal, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.

You may not:

  • NONCOMMERCIAL: You may not use the article for commercial purposes.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.