Efek Kurkumin terhadap Aktivitas Enzim Glutation Peroksidase Mitokondria Hati Tikus

Syamsudin Syamsudin; Suyatna FD; Ganiswarna S; Sadikin M

Syamsudin Syamsudin Universitas Pancasila
Suyatna FD Bagian Farmakologi Fakultas Kedokteran Universitas Indonesia
Ganiswarna S Bagian Farmakologi Fakultas Kedokteran Universitas Indonesia
Sadikin M Bagian Biokimia Fakultas Kedokteran Universitas Indonesia

Abstract

This study was conducted to prove the hypothesis that Curcumin could prevent the oxidative damage of rat liver mitochondria induced by t-butylhydroperoxide (t-BHP). The induction of 400 uMt-BHP reduced Gluthatione Peroxidase (GPx) activity from (0.29+0.03) to (0.04+0.01) umol/min/mg protein. Instillation of Curcumin of 1000 uM could elevate GPx activity to (0.16+0.002) umol/min/mg protein.

References

1. Kiso Y, Suzuki Y, Watanabe N. Antihepatotoxic principle of curcuma longa rhizomes. Planta Medica.1983;49:83.

2. Suyatna FD, Ganiswara S, Siswoyo K, Asikin N, Rosmiati H, Pringgo U. The effects of the curcuma againts paracetamol-induced liver damage in rats. Medical Journal of University of Indonesia. 1992;1(1):20.

3. Luft R. The development of mitochondrial medicine. Biochimica et Biophysica Acta. 1995;(1271):1-6.

4. Reed DJ, Gogvadze V, Laffanchi R Jones D, Lash, editors. Assesment of glutathione as a measure of cell injury in mitochondrial dysfunction. USA: Academic Press Inc.; 1993. p. 219-225.

5. Richter C, Gogvadze V, Laffanchi R. Oxidants in mitochondrial from physiology to disease. Biochimica et Biophysica Acta. 1995;(1272):67.

6. Halliwel B, Gutterdge JMC. Free radicals in biology and medicine. 3rded. Oxford: Oxford University Press; 1998. p. 22-24.

7. Toward NR, Dixon H, Kellerman GM. Biogenesis of mitochondrial, the sensitive of rat liver mitochondria to antibiotics: a phylogenetic difference between a mammalian system and yeast. Arch Biochem Biophysic. 1972;151:361.

8. Cohen G, Hochstein P. Glutathione peroxidase: the primary agent for the elimination of hydrogen peroxide in erythrocytes. Biochemistry. 1963;(2):1420.

9. Marinho, Antunes, Pinto R. Role of glutathione peroxidase and phospholipid hydroperoxide. Free Radical Biology and Medicine. 1997;22(5):888.

10. Fuller G, Shields D. Moleculer basis of medical cell biology. 1sted. USA: Prentice Hall International Inc; 1998.p. 100.

11. Djohan R. Efek kurkumin terhadap kerusakan mitokondria hati tikus yang ditimbulkan oleh butilhidroperoksida tersier [tesis). Jakarta: Universitas Indonesia; 1999.

12. Van der Goot H. The chemistry and qualitative structure-activity relationship of curcumin. Disampaikan pada International Symposium on Curcumin Pharmacochemistry (ISCP) 1995. Yogyakarta.

13. Meister A. Mitochondrial changes associated with glutathione deficiency. Biochimica et Biophysica Acta. 1995; 22.

14. Rendina G. Experimental methods in modern biochemistry. p. 284

15. Lash LH, Sall J, Jones D, Lash LH, editors. Mitochondrial isolation from liver and kidney, strategy techniques and criteria for purity in mitochondrial dysfunction. USA: Academic Press Inc; 1993. p. 8-21.