Pengaruh Penambahan Zat-zat Aditif pada Aktivitas Protease Bacillus megaterium DSM 319

  • Sumaryanto Sumaryanto PPKIT-BPPT
  • Deden RW Universitas Pancasila
  • Mahyudin A. Rahman Pusat Pengkajian dan Penerapan Teknologi Bioindustri

Abstract

Bacillus megaterium DSM 319 is a microbial neutral protease producer, which is usually used for food and non food (especially for dehairing and bating processes) industries. It was shown in this experiment, that the addition of some additive substances such as sodium benzoat, ammonium quarterner, calcium chloride and zeolite tested as a stabilizer of the ability for protease enzyme activities maintain comparing with control the application for the leather process was sign the difference performance. By using varian analysis and Duncan methode analysis at the 4 additif substances above from 0%, 0.5%, 1%, and 2% can be seen that the highest activity of protease reached at 0.862 U with sodium benzoat and the lowest activity at 0.636 U with zeolite.

References

1. Suhartono MT. Pengantar biokimia. Bogor: PAU Bioteknologi dan Lembaga Sumber Daya Informasi IPB; 1988.

2. Poedjadi A. Dasar-dasar biokimia. Jakarta: Universitas Indonesia; 1988.

3. Baas JE. Enzyme: their application and biochemical characterization. Surfactant Science. Series Library of Conggress Cataloging in Publication Data; 1990.

4. Bailey JE, DF Ollis. Biochemical engineering fundamental. 2nd Edition. New York: Mc Graw-Hill Book Company; 1988.

5. O'brien RT, LL Campbell. Purification and properties of a proteolytic enzyme from Bacillus stearothermophillus. J Archieves of Biochemistry and Biophysic. 1975.

6. Sukarno K, Takahashi, M Hatano, D Sakurai. Proteinase from the liver of neon flying squid: purification and properties. Bull Fac Hokkaido University. 1995.

7. Monsan, Combess. Stabilization of enzyme activity, the process of biotechnology. London: Online Publication Ltd; 1984.

8. Bergmeyer HV, M Grassl. Methods of enzymatic analysis. Vol II. Weinhein: Verleg Chemi; 1983.
How to Cite
SUMARYANTO, Sumaryanto; RW, Deden; RAHMAN, Mahyudin A.. Pengaruh Penambahan Zat-zat Aditif pada Aktivitas Protease Bacillus megaterium DSM 319. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 2, n. 2, p. 81-86, apr. 2004. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/632>. Date accessed: 05 nov. 2024.
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