Identification of α-glucosidase Enzyme Inhibitor Compound from Ethanol 96% Extract of Yakon Leaves (Smallanthus sonchifolius [Poepp.& Endl.] H. Robinson)

  • Zuhelmi Aziz Universitas Pancasila
  • Farhana Hanida Al Qisthi Universitas Pancasila
  • Nancy Dewi Yuliana Faculty of Argicultur Technology, Institut Pertanian Bogor
  • Partomuan Simanjuntak Pusat Penelitian Bioteknologi-LIPI

Abstract

The leaves of yacon (Smallanthussonchi folius [Poepp. &Endl.] H. Robinson) or in Indonesia known as “Daun insulin” (Insulin leaf), scientifically has various medicinal properties and one of them as antidiabetic. The aim of this research is to know α-glucosidase enzyme inhibitor compound in the ethanol 96% extract of yacon leaves. Fractionation of ethanol extract subjected to column chromatography (SiO2; dichloromethane-methanol-water = 5 : 5 : 1) gave 5 fractions (Fr -1 ~Fr – 5). The results of the -glucosidase enzyme inhibition activity test in vitro against the five fractions obtained the 3rd fraction as the most active of 87.84 %. Purification of the 3rd fraction with preparative thin layer chromatography (SiO2; n-BuOH-ethanol-water = 4 : 1 : 2.2) gave pure isolate as Fr 3-1. Identification of pure isolates Fr 3-1 was carried out by interpreted spectra of Ultraviolet, Fourier Transform-Infra Red, Mass Spectra (from LC-MS) and compared data on the chemical shift of the Nuclear Magnetic Resonance (proton and carbon) and determined as Nystose (GF3) compound. The results of the -glucosidase enzyme inhibition activity showed that      Fr 3-1 has IC50 of 21.36 ppm

References

1. International Diabetes Federation. IDF Diabetes Atlas 7th Edition. Brussels: International Diabetes Federation. http://www. diabetes atlas.org. 2015.

2. Depkes Republik Indonesia. Tahun 2030 prevalensi diabetes melitus di Indonesia mencapai 21,3 Juta orang. Kementrian Kesehatan Republik Indonesia. 2009; 1–2

3. Merlina M. Barabara/ Rani Nossar. Peluang Usaha: Budidaya Tanaman Obat Diabetes Mudah, Tanam Saja di Tempat Lembab. 2015. Diambil dari: http://www.tribunnews.com/bisnis/2015/09/03/peluang-usaha-budidaya-tanaman-obat-diabetes-mudah-tanam-saja-di-tempat-lembab. Diakses 21 Maret 2016.

4. Baroni S, Comar J F, Kemmelmeier F S, Mito M S, De Melo J O et al. . 2014. Beneficial effects of an hydroethanolic extract of smallanthus sonchifolius. Int J Pharm Bio Sci 2014 July ; 5 (3) : (P) 183 - 196

5. Lachman J, Fernández EC, Orsák M. 2003. Yacon [Smallanthus sonchifolius ( Poepp . et Endl .) H . Robinson] chemical composition and use – a review. ;2003(6):283–90.

6. Djamil R., Aziz Z., Virginia J., Penetapan dan identifikasi fraksi aktif penghambat enzim ɑ-glukosidase dari ekstrak etanol 96% daun yakon (Smallanthus sonchifolius (Poepp.) H. Robinson). Prosiding Seminar Nasional POKJANAS TOI ke-52 Tahun 2017 STIFAR Riau. 92 – 96

7. Djamil R, Winarti W, Simanjuntak P, Syamsudin. Standardization and α-glycosidase inhibition of extracts of Vatica pauciflora Blume stem barks and Smallanthus sonchifolius leaves. Journal of Pharmacognosy and Phytochemistry. 2014;3(4): 42-46.

8. Genta SB, Cabrera WM, Mercado MI, Grau A, Catalán CA, Sánchez SS. Hypoglycemic activity of leaf organic extracts from Smallanthus sonchifolius : Constituents of the most active fractions. Chemico-Biological Interactions 2010;185:143–52.

9. Grethel T. Choque Delgadoa, Rodolfo Thoméb, Dirce L. Gabrielb, Wirla M.S.C. Tamashirob, Glaucia M. Pastore. Yacon (Smallanthus sonchifolius)-derived fructooligosaccharides improves the immune parameters in the mouse. Nutritition Research 22 (2012) 884 – 892. Available online at www.sciencedirect .com. © 2012 Elsevier Inc.

10. Juliani. Nancy D. Yuliana, Slamet Budijanto, C. Hanny Wijaya, Alfi Khatib. Senayawa Inhibitor α-Glukosidase Dan Antioksidan Dari Kumis Kucing Dengan Pendekatan Metabolomik Berbasis FTIR. J. Teknol. dan Industri Pangan. 2016. Vol. 27(1): 17-30

11. Timmermans JW, De waard P, Tournuis H, Vliegenthaart. NMR spectroscopy of Nystose, Carbohydrated Res. 1993, 243, p. 379 – 384.

12. Bruyn AD and Loo JV. The Identification by 1H- and 13C-n.m.r. Spectroscopy of Sucrose, 1-Kestose, and Neokestose in Mixture Present in Plant Extracts. Carbohydrate Res. 211 (1991) p. 131-136.

13. Shiomi N and Onodera S. The 13C-NMR Spectra of Inulo-oligosaccharides. Agric. Biol. Chem. (1990).54 (1), p 215-216.

14. Joy M. Campbell et al. Selected Fructooligosaccharide (1-Kestose, Nystose, and 1F-â-Fructofuranosylnystose) Composition of Foods and Feeds. J. Agric. Food Chem. 1997, 45, 3076−3082

15. Fukushi.E et al. NMR analysis of Oligosaccharides containing fructopyranoside. Dynamic Biochemistry, Process Biotecnology and Molecular Biology. 2009. Global Science Book. https://doi.org/10.1016/B978-0-444-89369-7.50023-3

16. Zambelli P, et al. Production of fructooligosaccharides by mycelium-bound transfructosylation activity present in Cladosporium cladosporioides and Penicilium sizovae. Process Biochem .2014. http://dx.doi.org/10.1016/j.procbio.2014.09.021
Published
2019-04-24
How to Cite
AZIZ, Zuhelmi et al. Identification of α-glucosidase Enzyme Inhibitor Compound from Ethanol 96% Extract of Yakon Leaves (Smallanthus sonchifolius [Poepp.& Endl.] H. Robinson). JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 17, n. 1, p. 21-26, apr. 2019. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/652>. Date accessed: 17 july 2019.
Section
Articles