Studi Pendahuluan Mikroba Endofitik Penghasil Senyawa Terpen dari Tanaman Artenisia annua

  • Titi Parwati Pusat Penelitian Bioteknologi-LIPI
  • Bustanussalam Bustanussalam Pusat Penelitian Bioteknologi-LIPI
  • Partomuan Simanjuntak Pusat Penelitian Bioteknologi-LIPI

Abstract

Some endophytic microbes were isolated from medicinal plant Artemisia annua collected of Tawangmangu and Cibinong. Microbe isolate were made on corn meal malt agur supplemented with chloramphenicol and nutrient agar supplemented with nystatin from tissue subjected after the triple surface sterilization procedure. The potencies of these isolates to produce-terpene compounds were screenned by phytochemistry analysis, thin layer chromatography (tlc) and high performance liquid chromatography (hplc) after fermentation in four different medium. The results showed that 6 isolates from 17 microbe isolates can produce terpene compounds.

References

1. Corroll, G.C., 1986. The biology of endophytes in plants with particular reference to woody perennials, dalam Fokkema N.J. Van den Hewel (Editor), Mycrobiology of the phyllosphere, Cambridge, Cambridge University Press, 205-222.

2. Chapela, I., and L. Boddy, 1989. Fungal colonization of atterched beech branch II. Spatial and temporal organization of communities arising from latent in varders in and functional, under different moisture regions. New Phytologist 110, 44-57

3. Petrini, O., 1991. Fungal endophytes of the leaves, p. 173-197. dalam Microbial ecology of leaves, J.H. Andrens (editor), Springer-Verlag, New York

4. Strobel, G., A. Stierle, and W.M. Hess. 1993. Taxol formation in Yew-Taxus, Plant Sciences, 92, 1 – 12.

5. Stierle, A., G. Strobel, dan A. Stierle, 1993. Taxol and Taxane Production by Taxomyces andreana, an Endophytic fungus on Pacific Yew, Sciences, 260, 214-216

6. Simanjuntak, P.. Titi Parwati, Bustanussalam, Titik K.Prana dan H. Shibuya. 2002 Biochemical character of endophyte microbes isolated from Cinchona plants. Proceeding in Biotechnology for sustainable utilization of biological resources in the tropics, JSPS-NCRT/ DOST/ LIPI/VCC Joint Seminar, Bangkok Nov., 7-9, 2001, hal. 356-359

7. Simanjuntak, P., Titi Parwati, Bustanussalam, Titik K. Prana dan H. Shibuya. 2002. Produksi alkaloida kuinina oleh beberapa mikroba endofit dengan penambahan zat induser. Majalah farmasi Indonesia 13(1), hal. 1-6

8.Tan, R.X., W.F. Zheng, and H.Q. Tang. 1998. Biologically active Substances from the genus Artemisia, Planta Medica, 64, 295 – 302

9. Ando, K., 2000. Isolation and Identification of tropical fungi imperfecti, dalam Workshop Isolasi dan identifikasi fungi imperfekti daerah tropis, Pusat Antar Universitas (PAU) Pangan dan Gizi, Universitas Gajah Mada, Yogyakarta. hal. 1 - 85.

10. Kobinata, K., and H. Osada, 2000. Exploitation and a rapid detection method of bioactive compounds from microorganism.
How to Cite
PARWATI, Titi; BUSTANUSSALAM, Bustanussalam; SIMANJUNTAK, Partomuan. Studi Pendahuluan Mikroba Endofitik Penghasil Senyawa Terpen dari Tanaman Artenisia annua. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 1, n. 1, p. 27-32, apr. 2003. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/666>. Date accessed: 05 nov. 2024.
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